Anti-KAP1 (phospho S824) 抗体 (ab70369)
Key features and details
- Rabbit polyclonal to KAP1 (phospho S824)
- Suitable for: ICC, WB, IHC-P, IP
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-KAP1 (phospho S824) antibody
KAP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to KAP1 (phospho S824) -
由来種
Rabbit -
アプリケーション
適用あり: ICC, WB, IHC-P, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to Human KAP1 (phospho S824).
Database link: Q13263 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70369の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC | (1) |
Use at an assay dependent concentration.
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| WB | (2) |
1/1000 - 1/5000. Detects a band of approximately 117 kDa (predicted molecular weight: 89 kDa).
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| IHC-P |
Use a concentration of 0.2 µg/ml.
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| IP |
Use at 2-5 µg/mg of lysate.
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| 特記事項 |
|---|
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ICC
Use at an assay dependent concentration. |
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WB
1/1000 - 1/5000. Detects a band of approximately 117 kDa (predicted molecular weight: 89 kDa). |
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IHC-P
Use a concentration of 0.2 µg/ml. |
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IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. -
組織特異性
Expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. -
パスウェイ
Protein modification; protein sumoylation. -
配列類似性
Belongs to the TRIM/RBCC family.
Contains 2 B box-type zinc fingers.
Contains 1 bromo domain.
Contains 1 PHD-type zinc finger.
Contains 1 RING-type zinc finger. -
ドメイン
The HP1 box is both necessary and sufficient for HP1 binding.
The PHD-type zinc finger enhances CEBPB transcriptional activity. The PHD-type zinc finger, the HP1 box and the bromo domain, function together to assemble the machinery required for repression of KRAB domain-containing proteins. Acts as an intramolecular SUMO E3 ligase for autosumoylation of bromodomain.
The RING-finger-B Box-coiled-coil/tripartite motif (RBCC/TRIM motif) is required for interaction with the KRAB domain of KRAB-zinc finger proteins. Binds four zinc ions per molecule. The RING finger and the N-terminal of the leucine zipper alpha helical coiled-coil region of RBCC are required for oligomerization.
Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. ATM-induced phosphorylation on Ser-824 represses sumoylation leading to the de-repression of expression of a subset of genes involved in cell cycle control and apoptosis in response to genotoxic stress. Dephosphorylation by the phosphatases, PPP1CA and PP1CB forms, allows sumoylation and expression of TRIM28 target genes.
Sumoylation/desumoylation events regulate TRIM28-mediated transcriptional repression. Sumoylation is required for interaction with CHD3 and SETDB1 and the corepressor activity. Represses and is repressed by Ser-824 phosphorylation. Enhances the TRIM28 corepressor activity, inhibiting transcriptional activity of a number of genes including GADD45A and CDKN1A/p21. Lys-554, Lys-779 and Lys-804 are the major sites of sumoylation. In response to Dox-induced DNA damage, enhanced phosphorylation on Ser-824 prevents sumoylation and allows de-repression of CDKN1A/p21. -
細胞内局在
Nucleus. Associated with centromeric heterochromatin during cell differentiation through CBX1. - Information by UniProt
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参照データベース
- Entrez Gene: 10155 Human
- Entrez Gene: 711982 Rhesus monkey
- Omim: 601742 Human
- SwissProt: Q13263 Human
- Unigene: 467408 Human
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別名
- E3 SUMO protein ligase TRIM28 antibody
- E3 SUMO-protein ligase TRIM28 antibody
- FLJ29029 antibody
see all
画像
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Immunocytochemistry - Anti-KAP1 (phospho S824) antibody (ab70369)
Detection of KAP1 (phospho S824) by ab70369 (1:200) in NBF-fixed HeLa cells by Immunocytochemistry. Cells were treated with etoposide (left) and untreated (right). Red fluorescent anti-rabbit IgG dylight 594 conjugated was used as a secondary antibody at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 (phospho S824) antibody (ab70369)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung cancer cells labelling KAP1 with ab70369 at 0.2 µg/mL.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 (phospho S824) antibody (ab70369)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of HeLa cells labelling KAP1 with ab70369 at 0.2 µg/mL. Cells were treated with etoposide (left) and untreated (right).
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Immunoprecipitation - Anti-KAP1 (phospho S824) antibody (ab70369)Detection of Human KAP1 (phospho S824) by Western Blot of Immunoprecipitates.
Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells that were mock treated (-) or treated with Etoposide (100 µM, 2h).
Detection: Chemiluminescence with an exposure time of 30 seconds.
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Immunocytochemistry - Anti-KAP1 (phospho S824) antibody (ab70369)Detection of KAP1 (phospho S824) by ab70369 (1:200) in NBF-fixed HeLa cells by Immunocytochemistry.
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Western blot - Anti-KAP1 (phospho S824) antibody (ab70369)All lanes : Anti-KAP1 (phospho S824) antibody (ab70369) at 0.1 µg/ml
Lane 1 : Whole cell lysate from asynchronous HeLa cells, treated with neocarzinostatin (NCS; 200 ng/ml, 30 minutes) at 50 µg
Lane 2 : Whole cell lysate from asynchronous HeLa cells at 50 µg
Lane 3 : Whole cell lysate from asynchronous HeLa cells, treated with neocarzinostatin (NCS; 200 ng/ml, 30 minutes) at 200 µg
Lane 4 : Whole cell lysate from asynchronous HeLa cells at 200 µg
Developed using the ECL technique.
Predicted band size: 89 kDa
Observed band size: 117 kDa why is the actual band size different from the predicted? -
Immunocytochemistry - Anti-KAP1 (phospho S824) antibody (ab70369)This image is courtesy of an Abreview submitted by Ms. Can Zhouab70369 staining KAP1 (phospho S824) (red) in Human U2OS cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were treated with 1 µM Etoposide for 3 hours prior to fixing. Cells were fixed with formaldehyde, permeabilized in 0.5% NP40 and blocked with 3% BSA for 2 hours at 21°C. Samples were incubated with primary antibody (1/1000 in PBS + 3% BSA) for 12 hours at 4°C. An Alexa Fluor®594-conjugated Mouse anti-rabbit IgG monoclonal (1/500) was used as the secondary antibody.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (64)
ab70369 は 64 報の論文で使用されています。
- Jiang H et al. Human Endonuclease ANKLE1 Localizes at the Midbody and Processes Chromatin Bridges to Prevent DNA Damage and cGAS-STING Activation. Adv Sci (Weinh) 10:e2204388 (2023). PubMed: 36825683
- Ao Y et al. Role of C-Terminal Phosphorylation of Lamin A in DNA Damage and Cellular Senescence. Cells 12:N/A (2023). PubMed: 36831305
- Zhang Q et al. APE1 promotes non-homologous end joining by initiating DNA double-strand break formation and decreasing ubiquitination of artemis following oxidative genotoxic stress. J Transl Med 21:183 (2023). PubMed: 36894994
- Heath J et al. POGZ promotes homology-directed DNA repair in an HP1-dependent manner. EMBO Rep 23:e51041 (2022). PubMed: 34758190
- Stagni V et al. DNA Damage Regulates the Functions of the RNA Binding Protein Sam68 through ATM-Dependent Phosphorylation. Cancers (Basel) 14:N/A (2022). PubMed: 36010841