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Anti-kan 抗体 [4B4D1] (ab60018)

Reviews (1)Q&A (2)References (13)
Western blot - Anti-kan antibody [4B4D1] (ab60018)

    Key features and details

    • Mouse monoclonal [4B4D1] to kan
    • Suitable for: ELISA, WB
    • Reacts with: Recombinant fragment
    • Isotype: IgG2a

    製品の概要

    • 製品名

      Anti-kan antibody [4B4D1]

    • 製品の詳細

      Mouse monoclonal [4B4D1] to kan

    • 由来種

      Mouse

    • アプリケーション

      適用あり: ELISA, WBmore details

    • 種交差性

      交差種: Recombinant fragment
      交差が予測される動物種: Escherichia coli

    • 免疫原

      Recombinant full length protein corresponding to kan.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab60018の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    ELISA
    Use at an assay dependent concentration.
    WB
    1/500 - 1/2000. Detects a band of approximately 30 kDa (predicted molecular weight: 29 kDa).
    特記事項
    ELISA
    Use at an assay dependent concentration.
    WB
    1/500 - 1/2000. Detects a band of approximately 30 kDa (predicted molecular weight: 29 kDa).

    ターゲット情報

    • 関連性

      Neomycin Phosphotransferase 2 (kan) confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin®, G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids.

    • 参照データベース

      • 別名

        • Aminoglycoside 3' phosphotransferase antibody
        • APH(3') II antibody
        • APH(3')II antibody
        • aphA antibody
        • EC 2.7.1.95 antibody
        • Kan antibody
        • Kanamycin kinase type II antibody
        • Kanamycin kinase, type VIII antibody
        • kanamycin resistance gene antibody
        • Neo antibody
        • Neomycin kanamycin phosphotransferase type II antibody
        • Neomycin kanamycin phosphotransferase type VIII antibody
        • Neomycin Phosphotransferase 2 antibody
        • Neomycin phosphotransferase II antibody
        • Neomycine phosphotransferase antibody
        • NPT II antibody
        • NPTII antibody
        see all

      画像

      参考文献 (13)

      ab60018 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab60018 は 13 報の論文で使用されています。

      • Ji T  et al. The thioesterase APT1 is a bidirectional-adjustment redox sensor. Nat Commun 14:2807 (2023). PubMed: 37198152
      • Xue Q  et al. The PtdIns3P phosphatase MtMP promotes symbiotic nitrogen fixation via mitophagy in Medicago truncatula. iScience 26:107752 (2023). PubMed: 37954141
      • Li X  et al. Dehydrin MtCAS31 promotes autophagic degradation under drought stress. Autophagy N/A:1-16 (2019). PubMed: 31362589
      • Kallehauge TB  et al. Ribosome profiling-guided depletion of an mRNA increases cell growth rate and protein secretion. Sci Rep 7:40388 (2017). PubMed: 28091612
      • Bentham MJ  et al. NS2 is dispensable for efficient assembly of hepatitis C virus-like particles in a bipartite trans-encapsidation system. J Gen Virol 95:2427-41 (2014). PubMed: 25024280
      View all Publications for this product

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-3 of 3 Abreviews or Q&A

      Western blot abreview for Anti-kan antibody [4B4D1]

       Inconclusive
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Arabidopsis thaliana Tissue lysate - whole (seedlings)
      Gel Running Conditions
      Reduced Denaturing (12)
      Loading amount
      100 µg
      Treatment
      protease inhibitor
      Specification
      seedlings
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Nico Dissmeyer

      Verified customer

      投稿 Aug 03 2015

      Question

      Our customer would like to purchase ab60018 to ICC-IF test and his sample is human cell.
      I have already offered information of the customer.
      Please arrange discount code.

      Best regards

      Read More
      Answer

      I am very pleased to hear you would like to accept our offer and test ab60018 in ICC-IF

      DISCOUNT CODE: #####
      Expiration date: ######

      This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forICC-IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

      For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

      Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

      The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

      Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

      Read More

      Question

      Dear technical team,

      Our customer would like to purchase ab33595 or ab60018 toIF test and his sample is human.
      ab33595, ab60018 were tested in human sample by references.
      Let me know if you can give discount code for this sample and application.

      Best regards,

      Read More
      Answer

      Thank you for your enquiry.

      I can confirm that to our knowledge, ab33595 and ab60018 have not yet been tested in ICC (immunocytochemistry staining of cell culture), IHC-Frozen sections (IHC-Fr) or IHC-Paraffin tissue sections (IHC-P).

      Therefore, I can offer a discount off a future purchase if you buy either ab33595 or ab60018 now, test it inICC-IF, IHC-Fr or IHC-P and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 freeprimary antibody.

      If you are interested in this offer, please follow these steps:

      1. Reply to this e-mail to let me know that you would like to proceed and which antibodythe customer would like to test. Please also confirm which application (ICC, IHC-Fr or IHC-P). I will then send a discount code. This code must be issued before purchasing the antibody so please wait for my reply before ordering.

      2. Purchasethe antibodyeither by phone, fax, or online (www.abcam.com).

      3. Test it in ICC, IHC-Fr or IHC-P.

      4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

      5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibody ordered and the discount code is valid for 4 months after issue.

      Please remember that submission of the Abreview is sufficient for the discount code to become active.

      We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even ifthe antibodyturns out to be unsuitable for ICC, IHC-Fr or IHC-P, you will still receive the discount on your next purchase after your Abreview has been submitted.

      The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

      Please let me know if you have any questions about this offer and I would be happy to help you further.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com