Anti-Kallistatin/PI-4 抗体 (ab154597)
Key features and details
- Rabbit polyclonal to Kallistatin/PI-4
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Kallistatin/PI-4 antibody
Kallistatin/PI-4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Kallistatin/PI-4 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human Kallistatin/PI-4 aa 115-354 (internal sequence).
Database link: P29622 -
ポジティブ･コントロール
- HCT116 whole cell lysate; HCT116 cells; Human colon carcinoma tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154597の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 49 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol, using 10mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
|
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 49 kDa. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol, using 10mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0). |
|
ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein. -
組織特異性
Expressed by the liver and secreted in plasma. -
配列類似性
Belongs to the serpin family. -
翻訳後修飾
The N-terminus is blocked. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 5267 Human
- Omim: 147935 Human
- SwissProt: P29622 Human
- Unigene: 719893 Human
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別名
- KAIN_HUMAN antibody
- KAL antibody
- Kallikrein binding protein antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Kallistatin/PI-4 antibody (ab154597)
Immunofluorescent analysis of methanol-fixed HCT116 cells labeling Kallistatin/PI-4 with ab154597 at 1/500 dilution.
The image in the lower panel was co-stained with Hoechst 33342. -
Western blot - Anti-Kallistatin/PI-4 antibody (ab154597)Anti-Kallistatin/PI-4 antibody (ab154597) at 1/1000 dilution + HCT116 whole cell lysate at 30 µg
Predicted band size: 49 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallistatin/PI-4 antibody (ab154597)Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling Kallistatin/PI-4 with ab154597 at 1/250 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab154597 は 1 報の論文で使用されています。
- Wu H et al. Kallistatin inhibits tumour progression and platinum resistance in high-grade serous ovarian cancer. J Ovarian Res 12:125 (2019). PubMed: 31884974