Anti-Kallikrein 8/KLK8 抗体 [EPR5752(2)] (ab150395)
![Western blot - Anti-Kallikrein 8/KLK8 antibody [EPR5752(2)] (ab150395)](https://www.abcam.co.jp/ps/products/150/ab150395/Images/ab150395-157016-anti-kallikrein-8-antibody-epr57522-western-blot.jpg "Western blot - Anti-Kallikrein 8/KLK8 antibody [EPR5752(2)] (ab150395)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5752(2)] to Kallikrein 8/KLK8
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-Kallikrein 8/KLK8 antibody [EPR5752(2)]
Kallikrein 8/KLK8 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR5752(2)] to Kallikrein 8/KLK8 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human Kallikrein 8/KLK8 aa 200-300 (C terminal). The exact sequence is proprietary.
-
ポジティブ･コントロール
- Jurkat, Human fetal brain, A431, and HeLa whole cell lysate (ab150035). Human endometrial carcinoma tissue.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5752(2) -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab150395の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
|
|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 28 kDa. |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
追加情報
Is unsuitable for Flow Cyt or ICC/IF.
ターゲット情報
-
機能
Serine protease which is capable of degrading a number of proteins such as casein, fibrinogen, kininogen, fibronectin and collagen type IV. Also cleaves L1CAM in response to increased neural activity. Induces neurite outgrowth and fasciculation of cultured hippocampal neurons. Plays a role in the formation and maturation of orphan and small synaptic boutons in the Schaffer-collateral pathway, regulates Schaffer-collateral long-term potentiation in the hippocampus and is required for memory acquisition and synaptic plasticity. Involved in skin desquamation and keratinocyte proliferation. Plays a role in the secondary phase of pathogenesis following spinal cord injury. -
組織特異性
Isoform 1 is predominantly expressed in the pancreas. Isoform 2 is expressed in adult brain and hippocampus. Isoform 1 and isoform 2 are found in fetal brain and placenta. Detected in salivary gland, uterus, thymus, breast, testis and kidney but not in spleen, liver, lung or normal ovarian tissue. Displays an 11.5-fold increase in Alzheimer disease hippocampus compared to controls and is overexpressed in some ovarian carcinomas. Expressed at low levels in normal skin while high levels are found in psoriasis vulgaris, seborrheic keratosis, lichen planus and squamous cell carcinoma skin samples. Expressed in the keratinocytes. -
配列類似性
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain. -
細胞内局在
Secreted. Cytoplasm. Shows a cytoplasmic distribution in the keratinocytes. - Information by UniProt
-
参照データベース
- Entrez Gene: 11202 Human
- Omim: 605644 Human
- SwissProt: O60259 Human
- Unigene: 104570 Human
-
別名
- Brain serine protease 1 antibody
- BSP1 antibody
- hK8 antibody
see all
画像
-
Western blot - Anti-Kallikrein 8/KLK8 antibody [EPR5752(2)] (ab150395)All lanes : Anti-Kallikrein 8/KLK8 antibody [EPR5752(2)] (ab150395) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Human fetal brain cell lysate
Lane 3 : A431 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit
Predicted band size: 28 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 8/KLK8 antibody [EPR5752(2)] (ab150395)](https://www.abcam.co.jp/ps/products/150/ab150395/Images/ab150395-157015-anti-kallikrein-8-antibody-epr57522-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 8/KLK8 antibody [EPR5752(2)] (ab150395)Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labelling Kallikrein 8/KLK8 with ab150395 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Anti-Kallikrein 8/KLK8 antibody [EPR5752(2)] (ab150395)](https://www.abcam.co.jp/ps/products/150/ab150395/Images/ab150395-3-benefits-of-recombinant-antibodies.png)
Anti-Kallikrein 8/KLK8 antibody [EPR5752(2)] (ab150395)
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (6)
ab150395 は 6 報の論文で使用されています。
- Du JK et al. A novel role of kallikrein-related peptidase 8 in the pathogenesis of diabetic cardiac fibrosis. Theranostics 11:4207-4231 (2021). PubMed: 33754057
- Hua Q et al. Upregulation of KLK8 Predicts Poor Prognosis in Pancreatic Cancer. Front Oncol 11:624837 (2021). PubMed: 34395235
- Hua Q et al. KLK8 promotes the proliferation and metastasis of colorectal cancer via the activation of EMT associated with PAR1. Cell Death Dis 12:860 (2021). PubMed: 34552064
- Lim SC et al. Extracellular acidity-induced expression of Kallikrein-related peptidases 7 and 8 is involved in increased invasiveness of gastric cancer cells. Oncol Rep 43:1705-1713 (2020). PubMed: 32323843
- Aseervatham J & Ogbureke KUE Effects of DSPP and MMP20 Silencing on Adhesion, Metastasis, Angiogenesis, and Epithelial-Mesenchymal Transition Proteins in Oral Squamous Cell Carcinoma Cells. Int J Mol Sci 21:N/A (2020). PubMed: 32630820
- Lei S et al. Expression and clinical significance of KLK5-8 in endometrial cancer. Am J Transl Res 11:4180-4191 (2019). PubMed: 31396327