Anti-Kallikrein 4 抗体 (ab181402)
Key features and details
- Rabbit polyclonal to Kallikrein 4
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Kallikrein 4 antibody
Kallikrein 4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Kallikrein 4 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human Kallikrein 4 (internal sequence). Peptide corresponds to 15 amino acids near the center. (NP_004908)
Database link: Q9Y5K2 -
ポジティブ･コントロール
- Human kidney tissue and tissue lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab181402の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 27 kDa).
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| ICC/IF |
Use a concentration of 20 µg/ml.
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| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 27 kDa). |
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ICC/IF
Use a concentration of 20 µg/ml. |
|
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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組織特異性
Expressed in prostate. -
関連疾患
Defects in KLK4 are the cause of amelogenesis imperfecta hypomaturation type 2A1 (AI2A1) [MIM:204700]. AI2A1 is an autosomal recessive defect of enamel formation. The disorder involves both primary and secondary dentitions. The teeth have a shiny agar jelly appearance and the enamel is softer than normal. Brown pigment is present in middle layers of enamel. -
配列類似性
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 9622 Human
- Omim: 603767 Human
- SwissProt: Q9Y5K2 Human
- Unigene: 218366 Human
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別名
- 7S nerve growth factor alpha chain antibody
- AI2A1 antibody
- Alpha-NGF antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 4 antibody (ab181402)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue, labeling Kallikrein 4 using ab181402 at 5 μg/mL.
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Western blot - Anti-Kallikrein 4 antibody (ab181402)Anti-Kallikrein 4 antibody (ab181402) at 1 µg/ml + Human kidney tissue lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 27 kDa
Observed band size: 31 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-Kallikrein 4 antibody (ab181402)Immunofluorescence analysis of Human kidney tissue, labeling Kallikrein 4 using ab181402 at 20 μg/mL.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (2)
ab181402 は 2 報の論文で使用されています。
- Apaolaza PS et al. Islet expression of type I interferon response sensors is associated with immune infiltration and viral infection in type 1 diabetes. Sci Adv 7:N/A (2021). PubMed: 33627420
- Cheng H et al. Circular RNA Circ_0025033 Promotes the Evolvement of Ovarian Cancer Through the Regulation of miR-330-5p/KLK4 Axis. Cancer Manag Res 12:2753-2765 (2020). PubMed: 32425594
