AB152136

Anti-Kallikrein 2/KLK2 抗体

Name: Anti-Kallikrein 2/KLK2 抗体 (ab152136) | Abcam
Brand: Abcam Limited
SKU: AB152136
Price: 76000 JPY
Availability: InStock

Anti-Kallikrein 2/KLK2 antibody

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(3 Publications)

Rabbit Polyclonal Kallikrein 2/KLK2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KLK2 aa 100-300.

別名を表示する

Kallikrein-2, Glandular kallikrein-1, Tissue kallikrein-2, hGK-1, KLK2

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 2/KLK2 antibody (AB152136)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 2/KLK2 antibody (AB152136)

Immunohistochemical analysis of paraffin-embedded human prostate and human salivary gland tissues labeling Kallikrein 2/KLK2 with ab152136 at 1/1000 dilution.

Corresponding RNA levels (RPKM) in the tissues are based on NCBI database.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Supplier Data

Western blot - Anti-Kallikrein 2/KLK2 antibody (AB152136)

15% SDS-PAGE

The signal was developed with Trident femto Western HRP Substrate.

All lanes:

Western blot - Anti-Kallikrein 2/KLK2 antibody (ab152136) at 1/500 dilution

All lanes:

22Rv1 (Human prostate carcinoma epithelial cell line) whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 29 kDa

Observed band size: 34 kDa

false

Supplier Data

Western blot - Anti-Kallikrein 2/KLK2 antibody (AB152136)

15% SDS-PAGE

All lanes:

Western blot - Anti-Kallikrein 2/KLK2 antibody (ab152136) at 1/500 dilution

All lanes:

Human prostate tissue extract at 5 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 29 kDa

Observed band size: 32 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

IHC-P, WB

applications

免疫原

Recombinant Fragment Protein within Human KLK2 aa 100-300. The exact immunogen used to generate this antibody is proprietary information.

P20151

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The protein Kallikrein 2 also known as KLK2 or kallikrein-related peptidase 2 is a serine protease with a molecular mass of approximately 28 kDa. KLK2 is predominantly expressed in the prostate gland and plays a role in the cleavage of specific protein substrates. It shares homology with other kallikrein family proteins and is involved in the enzymatic activity that regulates various physiological processes. This protein also carries the alternate names like ALSK24680 KLK2 protein and has a significant presence in seminal plasma.

Biological function summary

KLK2 is involved in the regulation of seminal fluid by activating other enzymes such as prostate-specific antigen (PSA) which is itself part of the kallikrein family. KLK2 functions in the liquefaction process of seminal fluid aiding in sperm motility. It exists as part of a proteolytic cascade complex which includes other kallikreins. The expression and activity of KLK2 are highly regulated by androgens in the prostate tissue indicating its importance in male reproductive health.

Pathways

KLK2 participates in the kallikrein-kinin system and is closely related to other kallikrein family members like KLK3 (PSA) in regulatory mechanisms critical for reproductive biology. KLK2 influences pathways such as the fibrinolysis system by activating urokinase-type plasminogen activator (uPA). Additionally KLK2 modulates pathways related to extracellular matrix degradation connecting with proteins such as matrix metalloproteinases (MMPs).

KLK2 has a strong association with prostate cancer often used as a biomarker alongside PSA for early detection and prognosis assessment. Elevated levels of KLK2 can indicate a progression of malignant disease in the prostate. Furthermore KLK2 may have connections to benign prostatic hyperplasia (BPH) a noncancerous enlargement of the prostate. Its expression levels and activity can influence disease development and it is considered in research targeting prostate-related health disorders.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin.

See full target information KLK2

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2400398 PubMed38958553

2024

UBX-390: A Novel Androgen Receptor Degrader for Therapeutic Intervention in Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Soohyun Lee,Hwa-Ryeon Kim,Yaejin Woo,Jiyoung Kim,Han Wool Kim,Ji Youn Park,Beomseon Suh,Yuri Choi,Jungmin Ahn,Je Ho Ryu,Jae-Seok Roe,Jaewhan Song,Song Hee Lee

Molecular oncology 18:1966-1979 PubMed38605607

2024

Manzamine A reduces androgen receptor transcription and synthesis by blocking E2F8-DNA interactions and effectively inhibits prostate tumor growth in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Dev Karan,Seema Dubey,Sumedha Gunewardena,Kenneth A Iczkowski,Manohar Singh,Pengyuan Liu,Angelo Poletti,Yeun-Mun Choo,Hui-Zi Chen,Mark T Hamann

The Journal of clinical investigation 133: PubMed37022795

2023

Long noncoding RNA HIKER regulates erythropoiesis in Monge's disease via CSNK2B.

Applications

Unspecified application

Species

Unspecified reactive species

Priti Azad,Dan Zhou,Hung-Chi Tu,Francisco C Villafuerte,David Traver,Tariq M Rana,Gabriel G Haddad

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Kallikrein 2/KLK2 抗体

Anti-Kallikrein 2/KLK2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 2/KLK2 antibody (AB152136)

Western blot - Anti-Kallikrein 2/KLK2 antibody (AB152136)

Western blot - Anti-Kallikrein 2/KLK2 antibody (AB152136)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (3)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise