AB251434

Anti-KA1 抗体 [EPR18322] - BSA and Azide free

Anti-KA1 antibody [EPR18322] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal KA1 antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

別名を表示する

GRIK, GRIK4, GluK4, Excitatory amino acid receptor 1, Glutamate receptor KA-1, EAA1, KA1

3 Images

Supplier Data

Western blot - Anti-KA1 antibody [EPR18322] - BSA and Azide free (AB251434)

This data was developed using ab205537, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-KA1 antibody [EPR18322] (<a href='/products/primary-antibodies/ka1-antibody-epr18322-ab205537'>ab205537</a>) at 1/1000 dilution

All lanes:

Human hippocampus lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 107 kDa

Observed band size: 107 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-KA1 antibody [EPR18322] - BSA and Azide free (AB251434)

This data was developed using ab205537, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-KA1 antibody [EPR18322] (<a href='/products/primary-antibodies/ka1-antibody-epr18322-ab205537'>ab205537</a>) at 1/1000 dilution

All lanes:

Rat brain lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 107 kDa

Observed band size: 107 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-KA1 antibody [EPR18322] - BSA and Azide free (AB251434)

This data was developed using ab205537, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-KA1 antibody [EPR18322] (<a href='/products/primary-antibodies/ka1-antibody-epr18322-ab205537'>ab205537</a>) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Mouse heart lysate at 10 µg

Lane 3:

Mouse kidney lysate at 10 µg

Lane 4:

Mouse spleen lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 107 kDa

Observed band size: 107 kDa

false

Exposure time: 3min

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR18322

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Mouse, Rat, Human

アプリケーション

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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製品の詳細

ab251434 is the carrier-free version of ab205537.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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出荷温度及び保存条件

製品の状態

Liquid

バッファー組成

pH: 7.2 - 7.4 Constituents: PBS

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

Do Not Freeze

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The KA1 domain also known as the Kinase Associated-1 domain is an important structural component in certain proteins. The KA1 domain exhibits a molecular mass of approximately 8-10 kDa depending on its specific protein context. It plays an important role in targeting and binding within the cell. The KA1 domain is expressed in various cell types particularly those in the brain and muscle tissue where it contributes to cellular signaling and function.

Biological function summary

The KA1 domain of proteins helps regulate several important cellular processes. It is often part of larger protein complexes where it assists in localization and anchoring to specific structures in the cell. The domain influences various cellular activities including the assembly of signaling molecules and interaction with phospholipids. By contributing to the regulation of protein kinases and phosphatases the KA1 domain maintains critical cellular functions across different cell types.

Pathways

The KA1 domain integrates into the regulation of mitogen-activated protein kinase (MAPK) pathways and protein kinase A (PKA) signaling. These pathways play essential roles in cell growth apoptosis and other signaling processes. The KA1 domain often collaborates with other proteins such as PKC (Protein Kinase C) to maintain proper signaling cascade function and ensure accurate cellular responses.

The KA1 domain's function can link to neurodegenerative diseases and cancer. Alterations in proteins containing the KA1 domain have been associated with unregulated cell growth or improper signaling cascades often seen in cancerous tissues. The KA1 domain also interacts with proteins like tau in neurons potentially impacting neurodegenerative conditions by affecting cell signaling and structure. These interactions highlight the KA1 domain's importance in contributing to disease pathways and inform therapeutic targets.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Ionotropic glutamate receptor that functions as a cation-permeable ligand-gated ion channel. Cannot form functional channels on its own (PubMed : 8263508). Shows channel activity only in heteromeric assembly with GRIK1, GRIK2 and GRIK3 subunits (By similarity).

See full target information GRIK4

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:26521 PubMed39489850

2024

GABPA inhibits tumorigenesis in clear cell renal cell carcinoma by regulating ferroptosis through ACSL4.

Applications

Unspecified application

Species

Unspecified reactive species

Yaqian Wu,Zonglong Wu,Mengfei Yao,Li Liu,Yimeng Song,Lulin Ma,Cheng Liu

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KA1 抗体 [EPR18322] - BSA and Azide free

Anti-KA1 antibody [EPR18322] - BSA and Azide free

Western blot - Anti-KA1 antibody [EPR18322] - BSA and Azide free (AB251434)

Western blot - Anti-KA1 antibody [EPR18322] - BSA and Azide free (AB251434)

Western blot - Anti-KA1 antibody [EPR18322] - BSA and Azide free (AB251434)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

バッファー組成

出荷温度

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (1)

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise