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リコンビナントRabMAb

Anti-JunD (phospho S100) + c-Jun (phospho S73) 抗体 [EPR16586] (ab178858)

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Western blot - Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858)
  • Dot Blot - Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858)
  • Western blot - Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858)
  • Western blot - Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858)
  • Western blot - Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858)
  • Western blot - Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858)
  • Immunoprecipitation - Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858)
  • Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16586] to JunD (phospho S100) + c-Jun (phospho S73)
  • Suitable for: Dot blot, IP, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586]
    JunD+c-Jun 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR16586] to JunD (phospho S100) + c-Jun (phospho S73)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Dot blot, IP, IHC-P, WBmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa untreated and treated with 250 ng/ml Anisomycin for 30 minutes whole cell lysates; NIH/3T3 untreated and treated with 250 ng/ml Anisomycin for 30 minutes whole cell lysates; RAW 264.7 and PC-12 whole cell lysates. IHC-P: Human tonsil, mouse testis and rat liver tissues. IP: HeLa treated with 250ng/ml Anisomycin for 30 minutes whole cell extract.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab178858の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Dot blot
1/1000.
IP
1/100.
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 40,45,48 kDa (predicted molecular weight: 36 kDa).
特記事項
Dot blot
1/1000.
IP
1/100.
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 40,45,48 kDa (predicted molecular weight: 36 kDa).

ターゲット情報

画像

  • Western blot - Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858)
    Western blot - Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858)
    All lanes : Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858) at 1/10000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with 250 ng/ml Anisomycin for 30 minutes whole cell lysate
    Lane 2 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG,(H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 36 kDa
    Observed band size: 40,45,48 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/dilution buffer: 5% NFDM/TBST.

    Per the blast, ab178858 could recognize JunD (phospho Ser100) with 100% homology. Multi-bands are due to c-Jun (phospho Ser73) & JunD (phospho Ser100).

  • Dot Blot - Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858)
    Dot Blot - Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858)

    Dot blot analysis of JunD (phospho S100) + c-Jun (phospho S73) peptide (Lane 1) and non-phospho peptide (Lane 2) labeled using ab178858 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

     

  • Western blot - Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858)
    Western blot - Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858)
    Lanes 1 & 4-5 : Anti-c-Jun antibody [E254] - ChIP Grade (ab32137)
    Lanes 2-3 : Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858) at 1/10000 dilution

    Lanes 1 & 3 & 5 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lanes 2 & 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with 250 ng/ml Anisomycin for 30 minutes whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG,(H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 36 kDa
    Observed band size: 40,45 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858)
    Western blot - Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858)
    All lanes : Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858) at 1/10000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with 250 ng/ml Anisomycin for 30 minutes whole cell lysate
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with 250 ng/ml Anisomycin for 30 minutes, whole cell lysate treated with Alkaline Phosphatase

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG,(H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 36 kDa
    Observed band size: 40,45 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858)
    Western blot - Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858)
    All lanes : Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858) at 1/10000 dilution

    Lane 1 : NIH/3T3 (Mouse embryo fibroblast cell line) treated with 250 ng/ml Anisomycin for 30 minutes whole cell lysate
    Lane 2 : Untreated NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG,(H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 36 kDa
    Observed band size: 40,45 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/dilution buffer: 5% NFDM/TBST.

    Per the blast, ab178858 could recognize JunD (phospho Ser100) with 100% homology. Multi-bands are due to c-Jun (phospho Ser73) & JunD (phospho Ser100).

  • Western blot - Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858)
    Western blot - Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858)
    All lanes : Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858) at 1/1000 dilution

    Lane 1 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG,(H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 36 kDa
    Observed band size: 40,45 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/dilution buffer: 5% NFDM/TBST.

    Per the blast, ab178858 could recognize JunD (phospho Ser100) with 100% homology. Multi-bands are due to c-Jun (phospho Ser73) & JunD (phospho Ser100).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling JunD (phospho S100) + c-Jun (phospho S73) with ab178858 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on lymphocytes and endothelial cells of Human tonsil is observed. Counter stained with Hematoxylin.

    Negative Control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling JunD (phospho S100) + c-Jun (phospho S73) with ab178858 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on spermatogoniums and Leydig cells of mouse testis is observed. Counter stained with Hematoxylin.

    Negative Control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling JunD (phospho S100) + c-Jun (phospho S73) with ab178858 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on bile duct epithelial cells while no staining on hepatocytes of rat liver is observed. Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858)
    Immunoprecipitation - Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858)

    JunD (phospho S100) + c-Jun (phospho S73) were immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma), treated with 250ng/ml Anisomycin for 30 minutes, whole cell extract with ab178858 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab178858 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as secondary antibody at 1/1500 dilution.
    Lane 1: HeLa, treated with 250ng/ml Anisomycin for 30 minutes, whole cell extract 
    Lane 2: PBS.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 10 seconds.

     

    Per the blast, ab178858 could recognize JunD (phospho Ser100) with 100% homology. Multi-bands are due to c-Jun (phospho Ser73) & JunD (phospho Ser100)

  • Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858)
    Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858)

参考文献 (2)

ab178858 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab178858 は 2 報の論文で使用されています。

  • Pan J  et al. Papillary Thyroid Carcinoma Landscape and Its Immunological Link With Hashimoto Thyroiditis at Single-Cell Resolution. Front Cell Dev Biol 9:758339 (2021). PubMed: 34805166
  • Shao P  et al. MicroRNA-205-5p regulates the chemotherapeutic resistance of hepatocellular carcinoma cells by targeting PTEN/JNK/ANXA3 pathway. Am J Transl Res 9:4300-4307 (2017). PubMed: 28979703

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