AB191603

Anti-JNK1 + JNK3 抗体 [EPR16797-194]

Anti-JNK1 + JNK3 antibody [EPR16797-194]

20ul selling size
RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal JNK1 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat, Dog, Cow, Xenopus tropicalis, Recombinant fragment, Recombinant fragment - Human samples. Cited in 1 publication.

別名を表示する

JNK1, PRKM8, SAPK1, SAPK1C, MAPK8, Mitogen-activated protein kinase 8, MAP kinase 8, MAPK 8, JNK-46, Stress-activated protein kinase 1c, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, SAPK1c

6 Images

Supplier Data

Immunoprecipitation - Anti-JNK1 + JNK3 antibody [EPR16797-194] (AB191603)

JNK1 + JNK3 were immunoprecipitated from 1mg of K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell extract with ab191603 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab191603 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : K562 whole cell extract 10 μg (Input).

Lane 2 : ab191603 IP in K562 whole cell extract.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab191603 in K562 whole cell extract.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603)

Observed band size: 46 kDa,54 kDa

false

Supplier Data

Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] (AB191603)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603) at 1/2000 dilution

Lane 1:

K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate at 20 µg

Lane 2:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 3:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 48 kDa

Observed band size: 46 kDa,54 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] (AB191603)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Mouse heart tissue lysate at 10 µg

Lane 3:

Mouse kidney tissue lysate at 10 µg

Lane 4:

Rat kidney tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 48 kDa

Observed band size: 46 kDa,54 kDa

false

Exposure time: 1min

Supplier Data

Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] (AB191603)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603) at 1/2000 dilution

Lane 1:

Neuro-2a (Mouse neuroblastoma cells) whole cell lysate at 10 µg

Lane 2:

Xenopus (X. tropicalis) muscle lysates at 10 µg

Lane 3:

MDCK (Canine kidney cell line) whole cell lysate at 10 µg

Lane 4:

MDBK (Bovine kidney cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 48 kDa

Observed band size: 46 kDa,54 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] (AB191603)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603) at 1/1000 dilution

Lane 1:

C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

Lane 2:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 3:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

Lane 4:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 48 kDa

Observed band size: 46 kDa,54 kDa

false

Exposure time: 30s

Supplier Data

Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] (AB191603)

Recombinant full length JNK1 protein contains aa1-427 with His-Tag®. Recombinant full length JNK2 protein contains aa1-424 with GST-tag and JNK3 protein contains aa1-464 with GST-tag.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] (ab191603) at 1/2000 dilution

Lane 1:

Full length human JNK1 recombinant protein at 0.01 µg

Lane 2:

Full length human JNK2 recombinant protein at 0.01 µg

Lane 3:

Full length human JNK3 recombinant protein at 0.01 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 48 kDa

Observed band size: 48 kDa,71 kDa

false

Exposure time: 3min

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR16797-194

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Cow, Dog, Human, Xenopus tropicalis

アプリケーション

IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Recombinant fragment": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant fragment - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The c-Jun N-terminal kinases 1 and 3 (JNK1 and JNK3) are members of the mitogen-activated protein kinase (MAPK) family also known as Stress-Activated Protein Kinases (SAPKs). JNK1 has a molecular mass of approximately 46-54 kDa while JNK3 has a mass of about 54 kDa. JNK1 is expressed in most tissues with high levels in heart brain and leukocytes whereas JNK3 is mainly expressed in the brain but also found in the testes and heart. These kinases play a role in signaling cascades that respond to stress stimuli such as cytokines ultraviolet radiation and reactive oxygen species.

Biological function summary

JNK1 and JNK3 regulate transcription factors and influence gene expression to modulate cellular processes. They are part of a complex network that includes other proteins like MAP kinase kinases (MKKs) which activate JNKs through dual phosphorylation. JNK1 and JNK3 control processes such as cell proliferation apoptosis and differentiation. Their activation can trigger the phosphorylation of c-Jun a component of the Activator Protein 1 (AP-1) transcription factor complex influencing cell fate decisions.

Pathways

JNK1 and JNK3 are involved in important signaling pathways such as the MAPK signaling pathway and the apoptosis signaling pathway. These pathways interact with other proteins like MKK4 and MKK7 which act upstream to activate JNKs. The JNK pathway helps the cell respond to stress and regulate apoptosis interacting with proteins such as Bcl-2 which is involved in many cellular responses including cell survival and death.

JNK1 and JNK3 have been associated with conditions such as neurodegenerative diseases and cancer. Abnormal activation of these kinases is linked to Alzheimer’s disease which involves protein misfolding and synaptic dysfunction and to various cancers due to their role in controlling cell death and proliferation. In these contexts they interact with disease-related proteins like tau in Alzheimer's disease and p53 in cancer affecting disease progression and therapeutic responses.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway (PubMed : 28943315). In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity (PubMed : 18307971). Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins (PubMed : 21856198). Loss of this interaction abrogates the acetylation required for replication initiation (PubMed : 21856198). Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1 (PubMed : 21364637). In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation (PubMed : 21095239). Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy (PubMed : 18570871). Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons (By similarity). In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone (By similarity). Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH (PubMed : 16581800, PubMed : 17296730, PubMed : 20027304). Phosphorylates the CLOCK-BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed : 22441692). Phosphorylates the heat shock transcription factor HSF1, suppressing HSF1-induced transcriptional activity (PubMed : 10747973). Phosphorylates POU5F1, which results in the inhibition of POU5F1's transcriptional activity and enhances its proteasomal degradation (By similarity). Phosphorylates JUND and this phosphorylation is inhibited in the presence of MEN1 (PubMed : 22327296). In neurons, phosphorylates SYT4 which captures neuronal dense core vesicles at synapses (By similarity). Phosphorylates EIF4ENIF1/4-ET in response to oxidative stress, promoting P-body assembly (PubMed : 22966201). Phosphorylates SIRT6 in response to oxidative stress, stimulating its mono-ADP-ribosyltransferase activity (PubMed : 27568560). Phosphorylates NLRP3, promoting assembly of the NLRP3 inflammasome (PubMed : 28943315). Phosphorylates ALKBH5 in response to reactive oxygen species (ROS), promoting ALKBH5 sumoylation and inactivation (PubMed : 34048572).. JNK1 isoforms display different binding patterns : beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.

See full target information MAPK8

追加のターゲット

MAPK8

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

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Applications

Unspecified application

Species

Unspecified reactive species

Zhaoxiao Yu,Zhikun Wu,Dejian Liu,Haoyu Liu,Yu Zhang,Yaqian Zheng,Yanhong Huang,Shumin Liao,Yu Wei,Wei Huang,Zhenyu Zhang,Xi Liu,Haiying Yu,Di Wang,Liang Li,Feng Long,Luyan Z Ma

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-JNK1 + JNK3 抗体 [EPR16797-194]

Anti-JNK1 + JNK3 antibody [EPR16797-194]

Immunoprecipitation - Anti-JNK1 + JNK3 antibody [EPR16797-194] (AB191603)

Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] (AB191603)

Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] (AB191603)

Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] (AB191603)

Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] (AB191603)

Western blot - Anti-JNK1 + JNK3 antibody [EPR16797-194] (AB191603)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

追加のターゲット

文献 (1)

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Abcam product promise