AB179461

Anti-JNK1 + JNK2 + JNK3 抗体 [EPR16797-211]

Name: Anti-JNK1 + JNK2 + JNK3 抗体 [EPR16797-211] (ab179461) | Abcam
Brand: Abcam Limited
SKU: AB179461
Price: 28000 JPY
Availability: InStock
Rating: 4 (8 reviews)

Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211]

20ul selling size
RabMAb
Recombinant
詳細を見る

(8 Reviews)

(326 Publications)

Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (ab179461) is a rabbit monoclonal antibody detecting JNK1 + JNK2 + JNK3 in Western Blot, Flow Cytometry (Intra), IP, ICC/IF. Suitable for Chicken, Cow, Dog, Human, Mouse, Rat, Monkey, Xenopus, Zebrafish.

- Biophysical QC for unrivalled batch-batch consistency
- Over 220 publications

別名を表示する

JNK1, PRKM8, SAPK1, SAPK1C, MAPK8, Mitogen-activated protein kinase 8, MAP kinase 8, MAPK 8, JNK-46, Stress-activated protein kinase 1c, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, SAPK1c, JNK3, JNK3A, PRKM10, SAPK1B, MAPK10, Mitogen-activated protein kinase 10, MAP kinase 10, MAPK 10, MAP kinase p49 3F12, Stress-activated protein kinase 1b, Stress-activated protein kinase JNK3, c-Jun N-terminal kinase 3, SAPK1b, JNK2, PRKM9, SAPK1A, MAPK9, Mitogen-activated protein kinase 9, MAP kinase 9, MAPK 9, JNK-55, Stress-activated protein kinase 1a, Stress-activated protein kinase JNK2, c-Jun N-terminal kinase 2, SAPK1a

9 Images

Flow Cytometry (Intracellular) - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (AB179461)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (AB179461)

Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling JNK1+JNK2+JNK3 with purified ab179461 at 1/180 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

Immunocytochemistry/ Immunofluorescence - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (AB179461)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (AB179461)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling JNK1+JNK2+JNK3 with ab179461 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Confocal image showing both cytoplasmic and nuclear staining on HeLa cells. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows : -
-ve control 1 : - ab179461 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2 : - ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

Supplier Data

Immunoprecipitation - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (AB179461)

JNK1+JNK2+JNK3 were immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell extract with ab179461 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab179461 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1 : Jurkat whole cell extract. Lane 2 : PBS instead of Jurkat whole cell extract.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

JNKs originate from three genes that yield ten isoforms through alternative mRNA splicing, including JNK1α1, JNK1β1, JNK2α1, JNK2β1 and JNK3α1, which represent the p46 isoforms, and JNK1α2, JNK1β2, JNK2α2, JNK2β2 and JNK3β2, which represent the p54 isoforms.

All lanes:

Immunoprecipitation - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (ab179461)

Predicted band size: 48 kDa

false

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (AB179461)

Formaldehyde-fixed, NP40 permeabilized Mouse Vascular smooth muscle cells stained for JNK1+JNK2+JNK3 (Green) using ab179461 at 1/200 dilution followed by a Donkey anti-rabbit Alex Fluor® 488 antibody at 1/500 dilution. The nuclear counterstain was DAPI (Blue).

Supplier Data

Western blot - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (AB179461)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (ab179461) at 1/20000 dilution

Lane 1:

K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysates at 20 µg

Lane 2:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates at 20 µg

Lane 3:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 48 kDa

Observed band size: 46 kDa,54 kDa

false

Supplier Data

Western blot - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (AB179461)

Human JNK1, JNK2 and JNK3 full length recombinant proteins are from commercial sources. JNK1 and JNK2 have a proprietary tag, JNK3 has a His tag.

Blocking/dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (ab179461) at 1/20000 dilution

Lane 1:

Human JNK3 full length recombinant protein containing a proprietary tag. at 0.01 µg

Lane 2:

Human JNK2 full length recombinant protein containing a proprietary tag. at 0.01 µg

Lane 3:

Human JNK1 full length recombinant protein containing a His tag. at 0.01 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 48 kDa

Observed band size: 48 kDa,71 kDa

false

Supplier Data

Western blot - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (AB179461)

Blocking/dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (ab179461) at 1/5000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Rat brain lysate at 10 µg

Lane 3:

Rat heart lysate at 10 µg

Lane 4:

RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) lysate at 10 µg

Lane 5:

PC-12 (Rat adrenal gland pheochromocytoma) lysate at 10 µg

Lane 6:

NIH/3T3 (Mouse embyro fibroblast cells) lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 48 kDa

Observed band size: 46 kDa,54 kDa

false

Supplier Data

Western blot - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (AB179461)

Blocking/dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (ab179461) at 1/5000 dilution

Lane 1:

Neuro-2a (Mouse neuroblastoma cells) whole cell lysates at 10 µg

Lane 2:

UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) whole cell lysates at 10 µg

Lane 3:

MDCK (Canine kidney cell line) whole cell lysates at 10 µg

Lane 4:

MDBK (Bovine kidney cell line) whole cell lysates at 10 µg

Lane 5:

COS-1 (African green monkey kidney fibroblast-like cell line) whole cell lysates at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 48 kDa

Observed band size: 46 kDa,54 kDa

false

Supplier Data

Western blot - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (AB179461)

Blocking/dilution buffer : 5% NFDM/TBST.

Zebrafish has only one JNK isoform, JNK1 with a MW of 44kDa. So there is only one band in Zebrafish.

All lanes:

Western blot - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (ab179461) at 1/1000 dilution

Lane 1:

Zebrafish lysate at 10 µg

Lane 2:

X. tropicalis lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 48 kDa

Observed band size: 46 kDa,54 kDa

false

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR16797-211

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Chicken, Cow, Dog, Human, Zebrafish, African green monkey, Xenopus tropicalis

アプリケーション

ICC/IF, WB, Flow Cyt (Intra), IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/180", "FlowCytIntra-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "African green monkey": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Chicken": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Cow": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Dog": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Recombinant full length protein - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Zebrafish": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

製品の詳細

What is this antibody validated in?
Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (ab179461) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF) in African green monkey, Chicken, Cow, Dog, Human, Mouse, Rat, Xenopus tropicalis, Zebrafish samples.

What is the molecular weight of JNK1 + JNK2 + JNK3?
Anti-JNK1 + JNK2 + JNK3 [EPR16797-211] (ab179461) specifically detects a band for JNK1 + JNK2 + JNK3 (UniProt: P45983) at a molecular weight of 48kDa.

Trusted by the scientific community
Anti-JNK1 + JNK2 + JNK3 [EPR16797-211] (ab179461) was first used in a scientific publication in 2014 and has been cited over 220 times in peer-reviewed journals.

Reviewed by scientists
Anti-JNK1 + JNK2 + JNK3 [EPR16797-211] (ab179461) has over 5 independent reviews from customers.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR16797-211] also available for your convenience: ab179461, Carrier free - ab225572, Alexa Fluor® 488 - ab315841, Alexa Fluor® 647 - ab315842

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

JNK1 JNK2 and JNK3 collectively known as c-Jun N-terminal kinases are important members of the mitogen-activated protein kinase (MAPK) family. JNK1 JNK2 and JNK3 have molecular weights of approximately 46 to 55 kDa with JNK1 being around 46 kDa JNK2 about 54 kDa and JNK3 also around 54 kDa. They are expressed differently across tissues: JNK1 and JNK2 are widely present in many tissues while JNK3 is mostly in brain heart and testis. These kinases primarily phosphorylate serine and threonine residues acting on various transcription factors including c-Jun to regulate cellular processes.

Biological function summary

These kinases function as significant regulators of cellular responses to stress and cytokines. They do not act alone but often form part of larger signaling complexes that include other MAPKs and various scaffold proteins. Their roles are substantial in cell differentiation proliferation apoptosis and migration. Precise regulation by JNK molecules influences these critical cellular events which play a role in maintaining homeostasis and response to external stresses.

Pathways

JNK proteins are central to the MAPK signaling pathway and are tightly linked to the MAPK/ERK pathway. Activation of the JNK pathways leads to the regulation of various other proteins including those in the apoptosis pathway such as Bcl-2 and Bax. These interactions focus on cellular stress response regulation and contribute to processes like inflammation stress-induced apoptosis and some metabolic processes.

JNKs have important roles in inflammatory diseases and neurodegenerative disorders such as Alzheimer's disease and Parkinson's disease. Their activity links to the regulation of pro-inflammatory cytokines and neuronal apoptosis. JNK1 and JNK2 are particularly connected to inflammatory responses whereas JNK3 with its expression in the brain has associations with neuronal death in neurodegenerative diseases. Modulating JNK activity may offer therapeutic approaches for these conditions targeting pathways involving proteins like TNF-alpha and AΒ-amyloid.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway (PubMed : 28943315). In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity (PubMed : 18307971). Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins (PubMed : 21856198). Loss of this interaction abrogates the acetylation required for replication initiation (PubMed : 21856198). Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1 (PubMed : 21364637). In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation (PubMed : 21095239). Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy (PubMed : 18570871). Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons (By similarity). In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone (By similarity). Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH (PubMed : 16581800, PubMed : 17296730, PubMed : 20027304). Phosphorylates the CLOCK-BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed : 22441692). Phosphorylates the heat shock transcription factor HSF1, suppressing HSF1-induced transcriptional activity (PubMed : 10747973). Phosphorylates POU5F1, which results in the inhibition of POU5F1's transcriptional activity and enhances its proteasomal degradation (By similarity). Phosphorylates JUND and this phosphorylation is inhibited in the presence of MEN1 (PubMed : 22327296). In neurons, phosphorylates SYT4 which captures neuronal dense core vesicles at synapses (By similarity). Phosphorylates EIF4ENIF1/4-ET in response to oxidative stress, promoting P-body assembly (PubMed : 22966201). Phosphorylates SIRT6 in response to oxidative stress, stimulating its mono-ADP-ribosyltransferase activity (PubMed : 27568560). Phosphorylates NLRP3, promoting assembly of the NLRP3 inflammasome (PubMed : 28943315). Phosphorylates ALKBH5 in response to reactive oxygen species (ROS), promoting ALKBH5 sumoylation and inactivation (PubMed : 34048572).. JNK1 isoforms display different binding patterns : beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.

See full target information MAPK8

追加のターゲット

MAPK9,MAPK10

style

left-column

文献 (326)

Recent publications for all applications. Explore the full list and refine your search

The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology : PubMed40947964

2025

Eupatilin alleviates right ventricular fibrosis in rats with pulmonary hypertension induced by monocrotaline.

Applications

Unspecified application

Species

Unspecified reactive species

Tonggang Zhu,Xue Xiao,Xue Li,Zhenkun Liu

Mediators of inflammation 2025:4932970 PubMed40918406

2025

Electroacupuncture Attenuates Hepatic Ischemia-Reperfusion Injury by Modulating the Esr1/TAK1-JNK/p38 Signaling Pathway in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaofang Fan,Wei Guo,Xiaodan Yang,Hao Zhang,Bruno Fink,Lingyu Hu,Xiaoguang Wang

Cellular and molecular life sciences : CMLS 82:269 PubMed40610735

2025

Cell division cycle protein 42-driven activation of the MKK3/6-p38 signaling pathway participates in cardiac remodeling in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Wen,Lin Xie,Quan-Wen Liu,Guan-Hui Yu,Xu-Hui Qiao,Yu-Chun Huang,Lu Wang,Xin Li,Li-Dan Wen,Xiao-Lei Wang,Jing He,Xin-Yu Xiao,Xiao-Xiao Zhao,Ling-Fang Wang,Hong-Bo Xin,Ke-Yu Deng

International journal of molecular sciences 26: PubMed40565375

2025

Isostrictiniin Alleviates LPS-Induced Acute Lung Injury via the Regulation of the Keap1-Nrf2/HO-1 and MAPK/NF-κB Signaling Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Wanting Ding,Yuan Sun,Wulipan Tuohudaali,Chenyang Li,Yuhan Yao,Jun Zhao

American journal of cancer research 15:1759-1776 PubMed40371147

2025

Prognostic value, biological role, and mechanisms of LCN2 in childhood acute lymphoblastic leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Tang,Yuan-Yuan Li,Lin-Jun Tan,Ju Gao,Zhi-Gui Ma,Xia Guo,Ling Gu,Han-Min Liu

Journal of biochemical and molecular toxicology 39:e70268 PubMed40269590

2025

N-Acety-L-Cysteine Alleviates Isoflurane-Triggered Neuronal Cell Parthanatos by Suppressing Reactive Oxygen Species Accumulation Through the Induction of c-Jun N-Terminal Kinase Signaling Pathway Inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Liu,Ya Liu,Xuedong Wang,Ming Liu,Yingying Wang,Chunsheng Feng,Meihua Piao

Cell reports. Medicine 6:102000 PubMed40056904

2025

LTA4H improves the tumor microenvironment and prevents HCC progression via targeting the HNRNPA1/LTBP1/TGF-β axis.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Yang,Xinyao Qiu,Yingcheng Yang,Jing Wu,Shan Wang,Bo Zheng,Jianmin Wu,Tao Zhou,Yangqianwen Zhang,Mixue Bai,Shuowu Liu,Zihan Zhao,Yani Zhang,Yixian Wang,Jinxia Bao,Mengye Wu,Dongdong Xue,Meiyu Bao,Ji Hu,Siyun Shen,Hongyang Wang,Lei Chen

Frontiers in immunology 16:1515605 PubMed39963142

2025

Resveratrol contributes to NK cell-mediated breast cancer cytotoxicity by upregulating ULBP2 through miR-17-5p downmodulation and activation of MINK1/JNK/c-Jun signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Bisha Ding,Jie Li,Jia-Lin Yan,Chun-Yan Jiang,Ling-Bo Qian,Jie Pan

Current research in toxicology 8:100211 PubMed39829635

2025

L-3-n-butylphthalide alleviates intermittent alcohol exposure-induced hypothalamic cell apoptosis via inhibiting the IRE1α-ASK1-JNK pathway in adolescent rats.

Applications

Unspecified application

Species

Unspecified reactive species

Shanyong Yi,Lai Wei,Bin Zhao,Zhijun Yao,Bin Yang

Molecular and cellular biochemistry 480:3147-3160 PubMed39690293

2024

Interleukin-22 promotes endometrial carcinoma cell proliferation and cycle progression via ERK1/2 and p38 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Shiqi Liu,Ruqian Zhao,Yuqin Zang,Pengzhu Huang,Qiaoling Zhang,Xiangqin Fan,Junyi Bai,Xingyu Zheng,Shuangshuang Zhao,Dan Kuai,Chao Gao,Yingmei Wang,Fengxia Xue

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-JNK1 + JNK2 + JNK3 抗体 [EPR16797-211]

Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211]

Flow Cytometry (Intracellular) - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (AB179461)

Immunocytochemistry/ Immunofluorescence - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (AB179461)

Immunoprecipitation - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (AB179461)

Immunocytochemistry/ Immunofluorescence - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (AB179461)

Western blot - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (AB179461)

Western blot - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (AB179461)

Western blot - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (AB179461)

Western blot - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (AB179461)

Western blot - Anti-JNK1 + JNK2 + JNK3 antibody [EPR16797-211] (AB179461)

関連する標識済み抗体及び組成の異なる製品 (4)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

追加のターゲット

文献 (326)

Abcam product promise