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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-JNK1 抗体 [EPR140(2)] (ab110724)

Reviews (1) Submit a question References (37)
Western blot - Anti-JNK1 antibody [EPR140(2)] (ab110724)
  • Western blot - Anti-JNK1 antibody [EPR140(2)] (ab110724)
  • Western blot - Anti-JNK1 antibody [EPR140(2)] (ab110724)
  • Western blot - Anti-JNK1 antibody [EPR140(2)] (ab110724)
  • Western blot - Anti-JNK1 antibody [EPR140(2)] (ab110724)
  • Anti-JNK1 antibody [EPR140(2)] (ab110724)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR140(2)] to JNK1
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-JNK1 antibody [EPR140(2)]
    JNK1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR140(2)] to JNK1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa, HEK-293, K562, C6, RAW 264.7 and MCF7 cell lysates.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab110724の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 48 kDa.

ターゲット情報

  • 機能

    Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
    JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.

  • 配列類似性

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.

  • ドメイン

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

  • 翻訳後修飾

    Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • AI849689 antibody
    • c Jun N terminal kinase 1 antibody
    • C-JUN kinase 1 antibody
    • c-Jun N-terminal kinase 1 antibody
    • EC 2.7.11.24 antibody
    • JNK 1 antibody
    • JNK antibody
    • JNK-46 antibody
    • JNK1A2 antibody
    • JNK21B1/2 antibody
    • MAP kinase 8 antibody
    • MAPK 8 antibody
    • mapk8 antibody
    • Mitogen activated protein kinase 8 antibody
    • Mitogen-activated protein kinase 8 antibody
    • MK08_HUMAN antibody
    • p54 gamma antibody
    • Prkm8 antibody
    • Protein kinase JNK1 antibody
    • Protein kinase, mitogen-activated, 8 antibody
    • SAPK 1 antibody
    • SAPK gamma antibody
    • SAPK1 antibody
    • Stress-activated protein kinase 1 antibody
    • Stress-activated protein kinase JNK1 antibody
    see all

画像

  • Western blot - Anti-JNK1 antibody [EPR140(2)] (ab110724)
    Western blot - Anti-JNK1 antibody [EPR140(2)] (ab110724)
    All lanes : Anti-JNK1 antibody [EPR140(2)] (ab110724) at 1/1000 dilution

    Lane 1 : Wild-type U-2 OS cell lysate
    Lane 2 : MAPK8 knockout U-2 OS cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 42-48 kDa why is the actual band size different from the predicted?



    False colour image of Western blot: Anti-JNK1 antibody [EPR140(2)] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab110724 was shown to bind specifically to JNK1. A band was observed at 42/48 kDa in wild-type U-2 OS cell lysates with no signal observed at this size in mapk8 knockout cell line ab277181 (knockout cell lysate ab277223). To generate this image, wild-type and mapk8 knockout U-2 OS cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

  • Western blot - Anti-JNK1 antibody [EPR140(2)] (ab110724)
    Western blot - Anti-JNK1 antibody [EPR140(2)] (ab110724)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: JNK1 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: MCF7 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab110724 observed at 46 and 54 kDa. Red - loading control, ab8226, observed at 42 kDa.

    ab110724 (unpurified) was shown to specifically react with JNK1 when JNK1 knockout samples were used. Wild-type and ProteinX knockout samples were subjected to SDS-PAGE. ab110724 and ab8226 (loading control to beta actin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-JNK1 antibody [EPR140(2)] (ab110724)
    Western blot - Anti-JNK1 antibody [EPR140(2)] (ab110724)
    All lanes : Anti-JNK1 antibody [EPR140(2)] (ab110724) at 1/2000 dilution

    Lane 1 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
    Lane 2 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 3 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 48 kDa
    Observed band size: 46,54 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-JNK1 antibody [EPR140(2)] (ab110724)
    Western blot - Anti-JNK1 antibody [EPR140(2)] (ab110724)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: JNK1 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: MCF7 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green).

    Green - target observed at 46 and 54 kDa. Red - loading control, ab8226, observed at 42 kDa.

    This western blot image is a comparison between ab110724 and a competitor's top cited mouse monoclonal antibody.

     

  • Western blot - Anti-JNK1 antibody [EPR140(2)] (ab110724)
    Western blot - Anti-JNK1 antibody [EPR140(2)] (ab110724)
    All lanes : Anti-JNK1 antibody [EPR140(2)] (ab110724) at 1/1000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 48 kDa

  • Anti-JNK1 antibody [EPR140(2)] (ab110724)
    Anti-JNK1 antibody [EPR140(2)] (ab110724)

参考文献 (37)

ab110724 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab110724 は 37 報の論文で使用されています。

  • Tian X  et al. c-Jun N-terminal kinase 2 suppresses pancreatic cancer growth and invasion and is opposed by c-Jun N-terminal kinase 1. Cancer Gene Ther 29:73-86 (2022). PubMed: 33526844
  • Lee JJ  et al. Galectin-3 Inhibitors Suppress Anoikis Resistance and Invasive Capacity in Thyroid Cancer Cells. Int J Endocrinol 2021:5583491 (2021). PubMed: 34035807
  • Zhu D  et al. Long non-coding RNA CASC2 restrains high glucose-induced proliferation, inflammation and fibrosis in human glomerular mesangial cells through mediating miR-135a-5p/TIMP3 axis and JNK signaling. Diabetol Metab Syndr 13:89 (2021). PubMed: 34446088
  • Li Z & Chen B DUSP4 alleviates LPS-induced chondrocyte injury in knee osteoarthritis via the MAPK signaling pathway. Exp Ther Med 22:1401 (2021). PubMed: 34650647
  • Liang Y  et al. Biological evaluation of linalool on the function of blood vessels. Mol Med Rep 24:N/A (2021). PubMed: 34713293
View all Publications for this product

レビューと Q&A

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Western blot abreview for Anti-JNK1 antibody [EPR140(2)]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Cat Cell lysate - whole cell (synovial)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
10 µg
Treatment
siRNA for scramble control, JNK1 and JNK2
Specification
synovial
Blocking step
(agent) for 50 minute(s) · Concentration: 100% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. 令 中野

Verified customer

投稿 Jun 30 2017

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