AB307426

Anti-JLP 抗体 [EPR25121-32]

Anti-JLP antibody [EPR25121-32]

20ul selling size
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal JLP antibody. Suitable for IP, WB and reacts with Human, Rat, Mouse samples.

別名を表示する

HSS, KIAA0516, MAPK8IP4, SYD1, HLC6, SPAG9, C-Jun-amino-terminal kinase-interacting protein 4, JIP-4, JNK-interacting protein 4, Cancer/testis antigen 89, Human lung cancer oncogene 6 protein, JNK-associated leucine-zipper protein, Mitogen-activated protein kinase 8-interacting protein 4, Proliferation-inducing protein 6, Protein highly expressed in testis, Sperm surface protein, Sperm-associated antigen 9, Sperm-specific protein, Sunday driver 1, CT89, HLC-6, JLP, PHET

4 Images

Supplier Data

Immunoprecipitation - Anti-JLP antibody [EPR25121-32] (AB307426)

JLP was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell) whole cell lysate 10 ug with ab307426 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307426 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : 293T (human embryonic kidney epithelial cell) whole cell lysate 10 ug
Lane 2 : ab307426 IP in 293T whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab307426 in 293T whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds

Lysates were freshly made and used for IP immediately to minimize protein degradation.

All lanes:

Immunoprecipitation - Anti-JLP antibody [EPR25121-32] (ab307426) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) whole cell lysate 10 μg

Lane 2:

293T whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 190 kDa

false

Exposure time: 3s

Supplier Data

Western blot - Anti-JLP antibody [EPR25121-32] (AB307426)

Blocking and diluting buffer and concentration : 5% NFDM/TBSTNegative control : A-172 (PMID : 23696027). Exposure time : 70 seconds

All lanes:

Western blot - Anti-JLP antibody [EPR25121-32] (ab307426) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate 20 μg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate 20 μg

Lane 3:

A-172 (human brain glioblastoma cell ) whole cell lysate 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 190 kDa

false

Exposure time: 70s

Supplier Data

Western blot - Anti-JLP antibody [EPR25121-32] (AB307426)

Blocking and diluting buffer and concentration : 5% NFDM/TBSTLysates were freshly made and used for Western blotting immediately to minimize protein degradation. Exposure time : 15 seconds

All lanes:

Western blot - Anti-JLP antibody [EPR25121-32] (ab307426) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate 20 μg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate 20 μg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 190 kDa

false

Exposure time: 15s

Supplier Data

Western blot - Anti-JLP antibody [EPR25121-32] (AB307426)

Blocking and diluting buffer and concentration : 5% NFDM/TBSTLysates were freshly made and used for Western blotting immediately to minimize protein degradation. Exposure time : 48 seconds

All lanes:

Western blot - Anti-JLP antibody [EPR25121-32] (ab307426) at 1/1000 dilution

Lane 1:

Mouse testis tissue lysate 20 μg

Lane 2:

Rat testis tissue lysate 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 190 kDa

false

Exposure time: 48s

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR25121-32

アイソタイプ

IgG

キャリアフリー

交差種

Human, Mouse, Rat

アプリケーション

WB, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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製品の詳細

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

JLP also known as JNK-associated leucine zipper protein is a scaffolding protein involved in the regulation of several signaling pathways. It has a molecular weight of approximately 155 kDa. JLP is widely expressed throughout various tissues including brain heart and skeletal muscle. It plays a role in directing components of signaling pathways to specific subcellular locations and works closely with other signaling proteins such as JIP4 (C-jun N-terminal kinase-interacting protein 4).

Biological function summary

JLP helps organize and maintain signaling complexes within the cell. It serves as a scaffold that brings together MAPK signaling components facilitating signal transduction and enhancing specificity in cell signaling events. By doing so it alters cellular responses to external stimuli. JLP often functions alongside other scaffold proteins within larger signaling groups promoting efficient signal cascade transmission and enabling precise regulation of cellular activities.

Pathways

JLP is particularly important within the mitogen-activated protein kinase (MAPK) pathway and the c-Jun N-terminal kinase (JNK) pathway. It aids in the organization and activation of these pathways by binding to specific kinases and other scaffolding proteins. JLP interacts with proteins like JNK and JIP1 to oversee the transmission of stress-related signals affecting processes such as apoptosis and inflammation.

JLP has connections to cancer and neurological disorders. Its involvement in cell signaling makes it a critical player in cancer progression as it can influence cellular growth and survival mechanisms. Alterations in JLP expression or function can disturb normal signaling balance therefore contributing to tumorigenesis. Additionally JLP’s function in neuronal signaling pathways links it to neurodegenerative diseases where dysregulated signaling could lead to disease pathology. Its interactions with proteins like JNK highlight its potential role in disease development offering a target for therapeutic interventions.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module (PubMed : 14743216). Regulates lysosomal positioning by acting as an adapter protein which links PIP4P1-positive lysosomes to the dynein-dynactin complex (PubMed : 29146937). Assists PIKFYVE selective functionality in microtubule-based endosome-to-TGN trafficking (By similarity).

See full target information SPAG9

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-JLP 抗体 [EPR25121-32]

Anti-JLP antibody [EPR25121-32]

Immunoprecipitation - Anti-JLP antibody [EPR25121-32] (AB307426)

Western blot - Anti-JLP antibody [EPR25121-32] (AB307426)

Western blot - Anti-JLP antibody [EPR25121-32] (AB307426)

Western blot - Anti-JLP antibody [EPR25121-32] (AB307426)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

組合せ製品

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise