Anti-JDP2 抗体 (ab40916)
Key features and details
- Rabbit polyclonal to JDP2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-JDP2 antibody -
製品の詳細
Rabbit polyclonal to JDP2 -
由来種
Rabbit -
特異性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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アプリケーション
適用あり: WB, ICC/IFmore details
適用なし: IHC-P -
種交差性
交差種: Human
交差が予測される動物種: Rat
非交差種:
Mouse -
免疫原
Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human JDP2.
Immunogen の所有権に関して (Peptide available as ab41625.) -
ポジティブ･コントロール
- ab40916 gave a positive signal in WB on the following lysates: HeLa whole cell, HeLa nuclear, Jurkat whole cell, Jurkat nuclear, HepG2 nuclear, Du145 whole cell and LnCAP nuclear. ICC/IF: HepG2 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab40916の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
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| ICC/IF |
Use a concentration of 1 µg/ml.
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| 特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa). |
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ICC/IF
Use a concentration of 1 µg/ml. |
ターゲット情報
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機能
Component of the AP-1 transcription factor that represses transactivation mediated by the Jun family of proteins. Involved in a variety of transcriptional responses associated with AP-1 such as UV-induced apoptosis, cell differentiation, tumorigenesis and antitumogeneris. Can also function as a repressor by recruiting histone deacetylase 3/HDAC3 to the promoter region of JUN. May control transcription via direct regulation of the modification of histones and the assembly of chromatin. -
配列類似性
Belongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain. -
翻訳後修飾
Phosphorylation of Thr-148 by MAPK8 in response to different stress conditions such as, UV irradiation, oxidatives stress and anisomycin treatments. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 122953 Human
- Entrez Gene: 116674 Rat
- Omim: 608657 Human
- SwissProt: Q8WYK2 Human
- SwissProt: Q78E65 Rat
- Unigene: 196482 Human
- Unigene: 10721 Rat
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別名
- Jdp2 antibody
- JDP2_HUMAN antibody
- Jun dimerization protein 2 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-JDP2 antibody (ab40916)
ab40916 staining JDP2 in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab40916 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Western blot - Anti-JDP2 antibody (ab40916)All lanes : Anti-JDP2 antibody (ab40916) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : Jurkat nuclear extract lysate (ab14844)
Lane 5 :Hep G2 nuclear extract lysate (ab14660)
Lane 6 : Du145 (Human prostate carcinoma cell line) Whole Cell Lysate
Lane 7 : LNCaP (Human prostate adenocarcinoma cell line) Nuclear lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 19 kDa
Observed band size: 19 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab40916 は 6 報の論文で使用されています。
- Bhattacharya A et al. MUC1-C intersects chronic inflammation with epigenetic reprogramming by regulating the set1a compass complex in cancer progression. Commun Biol 6:1030 (2023). PubMed: 37821650
- Cao L et al. Genotoxic stress-triggered ß-catenin/JDP2/PRMT5 complex facilitates reestablishing glutathione homeostasis. Nat Commun 10:3761 (2019). PubMed: 31434880
- Kong X et al. Defining UHRF1 Domains that Support Maintenance of Human Colon Cancer DNA Methylation and Oncogenic Properties. Cancer Cell 35:633-648.e7 (2019). PubMed: 30956060
- Mansour MR et al. JDP2: An oncogenic bZIP transcription factor in T cell acute lymphoblastic leukemia. J Exp Med 215:1929-1945 (2018). PubMed: 29941549
- Tanigawa S et al. Jun dimerization protein 2 is a critical component of the Nrf2/MafK complex regulating the response to ROS homeostasis. Cell Death Dis 4:e921 (2013). WB ; Human . PubMed: 24232097
- Chiou SS et al. Control of Oxidative Stress and Generation of Induced Pluripotent Stem Cell-like Cells by Jun Dimerization Protein 2. Cancers (Basel) 5:959-84 (2013). PubMed: 24202329