Anti-Jarid2 抗体 (ab184152)
Key features and details
- Rabbit polyclonal to Jarid2
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Jarid2 antibody
Jarid2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Jarid2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment within Human Jarid2 aa 662-891. The exact sequence is proprietary.
Database link: Q92833 -
ポジティブ･コントロール
- Jarid2-transfected 293T whole cell lysate and Mouse fetal forebrain lysate; Jarid2-transfected HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab184152の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/20000. Predicted molecular weight: 138 kDa.
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
|
WB
1/1000 - 1/20000. Predicted molecular weight: 138 kDa. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Regulator of histone methyltransferase complexes that plays an essential role in embryonic development, including heart and liver development, neural tube fusion process and hematopoiesis. Acts by modulating histone methyltransferase activity and promoting the recruitment of histone methyltransferase complexes to their target genes. Binds DNA and mediates the recruitment of the PRC2 complex to target genes in embryonic stem cells. Does not have histone demethylase activity but regulates activity of various histone methyltransferase complexes. In embryonic stem cells, it associates with the PRC2 complex and inhibits trimethylation of 'Lys-27' of histone H3 (H3K27me3) by the PRC2 complex, thereby playing a key role in differentiation of embryonic stem cells and normal development. In cardiac cells, it is required to repress expression of cyclin-D1 (CCND1) by activating methylation of 'Lys-9' of histone H3 (H3K9me) by the GLP1/EHMT1 and G9a/EHMT2 histone methyltransferases. Also acts as a transcriptional repressor of ANF via its interaction with GATA4 and NKX2-5. Participates in the negative regulation of cell proliferation signaling. -
組織特異性
During embryogenesis, predominantly expressed in neurons and particularly in dorsal root ganglion cells. -
配列類似性
Contains 1 ARID domain.
Contains 1 JmjC domain.
Contains 1 JmjN domain. -
ドメイン
The ARID domain is required to target the PRC2 complex to its target genes.
The GSGFP motif is required for the interaction with SUZ12. -
細胞内局在
Nucleus. Colocalizes with the PRC2 complex on chromatin. - Information by UniProt
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参照データベース
- Entrez Gene: 3720 Human
- Entrez Gene: 16468 Mouse
- Omim: 601594 Human
- SwissProt: Q92833 Human
- SwissProt: Q62315 Mouse
- Unigene: 269059 Human
- Unigene: 25059 Mouse
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別名
- JARD2 antibody
- JARD2_HUMAN antibody
- JARID2 antibody
see all
画像
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Western blot - Anti-Jarid2 antibody (ab184152)All lanes : Anti-Jarid2 antibody (ab184152) at 1/20000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : Jarid2-transfected 293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 138 kDa5 % SDS-PAGE
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Western blot - Anti-Jarid2 antibody (ab184152)Anti-Jarid2 antibody (ab184152) at 1/1000 dilution + Mouse fetal forebrain lysate at 50 µg
Predicted band size: 138 kDa5 % SDS-PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-Jarid2 antibody (ab184152)Immunofluorescent analysis of paraformaldehyde fixed Jarid2 transfected (right) or non-transfected (left) HeLa cells, labeling Jarid2 using ab184152 at 1/500 dilution. Lower images costained with Hoechst 33342.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab184152 は論文での使用が確認できていません。