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Anti-Japanese encephalitis virus NS1 glycoprotein 抗体 [MV12/2/A5-1/6] (ab41651)

Submit a review Submit a question References (9)
Immunocytochemistry/ Immunofluorescence - Anti-Japanese encephalitis virus NS1 glycoprotein antibody [MV12/2/A5-1/6] (ab41651)
  • Western blot - Anti-Japanese encephalitis virus NS1 glycoprotein antibody [MV12/2/A5-1/6] (ab41651)

Key features and details

  • Mouse monoclonal [MV12/2/A5-1/6] to Japanese encephalitis virus NS1 glycoprotein
  • Suitable for: IHC-FoFr, Flow Cyt, WB, ICC/IF, ELISA
  • Reacts with: Japanese encephalitis virus
  • Isotype: IgG3

製品の概要

  • 製品名

    Anti-Japanese encephalitis virus NS1 glycoprotein antibody [MV12/2/A5-1/6]
    Japanese encephalitis virus NS1 glycoprotein 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [MV12/2/A5-1/6] to Japanese encephalitis virus NS1 glycoprotein

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-FoFr, Flow Cyt, WB, ICC/IF, ELISAmore details

  • 種交差性

    交差種: Japanese encephalitis virus

  • 免疫原

    Full length native protein (purified) corresponding to Japanese encephalitis virus Japanese encephalitis virus NS1 glycoprotein.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.1% Proclin 150
    Constituents: 10% BSA, 89.9% RPMI 1640
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • ポリ/モノ

    モノクローナル

  • クローン名

    MV12/2/A5-1/6

  • アイソタイプ

    IgG3

  • 軽鎖の種類

    kappa

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab41651の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-FoFr
Use at an assay dependent concentration. PubMed: 19635909
Flow Cyt
Use at an assay dependent concentration. PubMed: 20581148

ab18392 - Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.
WB
1/50 - 1/100. Use under non reducing condition. Predicted molecular weight: 46 kDa.
ICC/IF
1/5 - 1/20.
ELISA
Use at an assay dependent concentration.
特記事項
IHC-FoFr
Use at an assay dependent concentration. PubMed: 19635909
Flow Cyt
Use at an assay dependent concentration. PubMed: 20581148

ab18392 - Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.
WB
1/50 - 1/100. Use under non reducing condition. Predicted molecular weight: 46 kDa.
ICC/IF
1/5 - 1/20.
ELISA
Use at an assay dependent concentration.

ターゲット情報

  • 関連性

    The Japanese encephalitis viral genome encodes 7 non-structural proteins NS1-NS5. NS1 contains N-linked carbohydrate chains at positions 130 and 207. It is not incorporated into the virion but exists in the host cell, on the cell surface and can also be extracellular.

  • 参照データベース

    • 別名

      • Japanese encephalitis non-structural protein 1 antibody
      • Japanese encephalitis NS1 antibody
      • Japanese encephalitis virus non-structural protein 1 antibody

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-Japanese encephalitis virus NS1 glycoprotein antibody [MV12/2/A5-1/6] (ab41651)
      Immunocytochemistry/ Immunofluorescence - Anti-Japanese encephalitis virus NS1 glycoprotein antibody [MV12/2/A5-1/6] (ab41651)
      Japanese encephalitis virus (Nakayama) infected PS clone D cells stained with ab41651 (green).
    • Western blot - Anti-Japanese encephalitis virus NS1 glycoprotein antibody [MV12/2/A5-1/6] (ab41651)
      Western blot - Anti-Japanese encephalitis virus NS1 glycoprotein antibody [MV12/2/A5-1/6] (ab41651)
      All lanes : Anti-Japanese encephalitis virus NS1 glycoprotein antibody [MV12/2/A5-1/6] (ab41651)

      Lane 1 : Japanese encephalitis virus infected C6/36 cell lysate (unheated)
      Lane 2 : Japanese encephalitis virus infected C6/36 cell lysate (boiled)

      Predicted band size: 46 kDa
      Observed band size: 46,92 kDa why is the actual band size different from the predicted?
      Additional bands at: 100 kDa (possible cleavage fragment), 50 kDa (possible cleavage fragment)



      This antibody recognsies 2 forms of NS1 - NS1 and NS1' (46 and 53 kDa respectively). NS1' is thought to be formed when NS1 is cleaved from NS2A at an alternative site. Both NS1 and NS1' exist as dimers in untreated samples but are dissociated into monomers when samples are boiled.

    参考文献 (9)

    ab41651 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab41651 は 9 報の論文で使用されています。

    • Sarkar R  et al. Japanese encephalitis virus capsid protein interacts with non-lipidated MAP1LC3 on replication membranes and lipid droplets. J Gen Virol 102:N/A (2021). PubMed: 33095129
    • Li XF  et al. Development of a chimeric Zika vaccine using a licensed live-attenuated flavivirus vaccine as backbone. Nat Commun 9:673 (2018). PubMed: 29445153
    • Sharma N  et al. Japanese Encephalitis Virus exploits the microRNA-432 to regulate the expression of Suppressor of Cytokine Signaling (SOCS) 5. Sci Rep 6:27685 (2016). WB ; Japanese encephalitis virus . PubMed: 27282499
    • Sharma N  et al. miR-146a suppresses cellular immune response during Japanese encephalitis virus JaOArS982 strain infection in human microglial cells. J Neuroinflammation 12:30 (2015). WB ; Japanese encephalitis virus . PubMed: 25889446
    • Sharma M  et al. Japanese encephalitis virus replication is negatively regulated by autophagy and occurs on LC3-I- and EDEM1-containing membranes. Autophagy 10:1637-51 (2014). PubMed: 25046112
    • Li XF  et al. A chimeric dengue virus vaccine using Japanese encephalitis virus vaccine strain SA14-14-2 as backbone is immunogenic and protective against either parental virus in mice and nonhuman primates. J Virol 87:13694-705 (2013). PubMed: 24109223
    • Zhang T  et al. Anti- Japanese-Encephalitis-Viral Effects of Kaempferol and Daidzin and Their RNA-Binding Characteristics. PLoS One 7:e30259 (2012). WB ; Japanese encephalitis virus . PubMed: 22276167
    • Aleyas AG  et al. Multifront Assault on Antigen Presentation by Japanese Encephalitis Virus Subverts CD8+ T Cell Responses. J Immunol : (2010). Flow Cyt . PubMed: 20581148
    • Aleyas AG  et al. Functional modulation of dendritic cells and macrophages by Japanese encephalitis virus through MyD88 adaptor molecule-dependent and -independent pathways. J Immunol 183:2462-74 (2009). IHC-FoFr . PubMed: 19635909

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