Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-Jagged1 抗体 [EPR4290] - BSA and Azide free (ab184785)

Submit a review Submit a question References (2)
Western blot - Anti-Jagged1 antibody [EPR4290] - BSA and Azide free (ab184785)
  • Western blot - Anti-Jagged1 antibody [EPR4290] - BSA and Azide free (ab184785)
  • Anti-Jagged1 antibody [EPR4290] - BSA and Azide free (ab184785)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4290] to Jagged1 - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-Jagged1 antibody [EPR4290] - BSA and Azide free
    Jagged1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR4290] to Jagged1 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details
    適用なし: ICC/IF or IHC-P

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HepG2 and HUVEC whole cell lysate.

  • 特記事項

    ab184785 is the carrier-free version of ab109536.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab184785の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 134 kDa.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 134 kDa.
追加情報
Is unsuitable for ICC/IF or IHC-P.

ターゲット情報

  • 機能

    Ligand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro).

  • 組織特異性

    Widely expressed in adult and fetal tissues. In cervix epithelium expressed in undifferentiated subcolumnar reserve cells and squamous metaplasia. Expression is up-regulated in cervical squamous cell carcinoma. Expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells.

  • 関連疾患

    Defects in JAG1 are the cause of Alagille syndrome type 1 (ALGS1) [MIM:118450]. Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. There are characteristic facial features and less frequent clinical involvement of the renal and vascular systems.
    Defects in JAG1 are a cause of tetralogy of Fallot (TOF) [MIM:187500]. TOF is a congenital heart anomaly which consists of pulmonary stenosis, ventricular septal defect, dextroposition of the aorta (aorta is on the right side instead of the left) and hypertrophy of the right ventricle. This condition results in a blue baby at birth due to inadequate oxygenation. Surgical correction is emergent.

  • 配列類似性

    Contains 1 DSL domain.
    Contains 15 EGF-like domains.

  • 発生段階

    Expressed in 32-52 days embryos in the distal cardiac outflow tract and pulmonary artery, major arteries, portal vein, optic vesicle, otocyst, branchial arches, metanephros, pancreas, mesocardium, around the major bronchial branches, and in the neural tube.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • AGS antibody
    • AHD antibody
    • AWS antibody
    • CD 339 antibody
    • CD339 antibody
    • CD339 antigen antibody
    • Headturner antibody
    • hJ1 antibody
    • Htu antibody
    • Jag 1 antibody
    • Jag1 antibody
    • JAG1_HUMAN antibody
    • Jagged 1 antibody
    • Jagged1 (Alagille syndrome) antibody
    • Jagged1 antibody
    • JAGL1 antibody
    • MGC104644 antibody
    • OTTHUMP00000030278 antibody
    • Protein jagged-1 antibody
    • Ser 1 antibody
    • Ser1 antibody
    • Serrate 1 antibody
    • Slalom antibody
    see all

画像

  • Western blot - Anti-Jagged1 antibody [EPR4290] - BSA and Azide free (ab184785)
    Western blot - Anti-Jagged1 antibody [EPR4290] - BSA and Azide free (ab184785)
    All lanes : Anti-Jagged1 antibody [EPR4290] (ab109536) at 1/10000 dilution

    Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
    Lane 2 : HUVEC (human umbilical vein endothelial cell), whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
    Lane 4 : MOLT-4 (human lymphoblastic leukemia T lymphoblast), whole cell lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 134 kDa
    Observed band size: 200,23 kDa why is the actual band size different from the predicted?


    Exposure time: 180 seconds


    Blocking buffer and concentration: 5% NFDM/TBST
    Diluting buffer and concentration: 5% NFDM /TBST

    Negative controls: Jurkat and MOLT-4 whole cell lysate (PMID: 30231940)

    200-kDa full length and 23-kDa C-terminal JAG1 are observed. The molecular weights are consistent with what has been described in the literature (PMID: 30890522, 30890522).

    This blot was developed using a high sensitivity ECL substrate.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109536).

  • Western blot - Anti-Jagged1 antibody [EPR4290] - BSA and Azide free (ab184785)
    Western blot - Anti-Jagged1 antibody [EPR4290] - BSA and Azide free (ab184785)
    All lanes : Anti-Jagged1 antibody [EPR4290] (ab109536) at 1/10000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : JAG1 knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 134 kDa
    Observed band size: 200 kDa why is the actual band size different from the predicted?



    Lanes 1 - 2: Merged signal (red and green). Green - ab109536 observed at 200 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab109536 was shown to recognize in wild-type HAP1 cells as signal was lost at the expected MW in JAG1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and JAG1 knockout samples were subjected to SDS-PAGE. Ab109536 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109536).

  • Anti-Jagged1 antibody [EPR4290] - BSA and Azide free (ab184785)
    Anti-Jagged1 antibody [EPR4290] - BSA and Azide free (ab184785)

参考文献 (2)

ab184785 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab184785 は 2 報の論文で使用されています。

  • Yoshida R  et al. The pathological significance of Notch1 in oral squamous cell carcinoma. Lab Invest 93:1068-81 (2013). Human . PubMed: 23938602
  • Yang W  et al. Ptpn11 deletion in a novel progenitor causes metachondromatosis by inducing hedgehog signalling. Nature 499:491-5 (2013). Mouse . PubMed: 23863940

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

There are currently no Customer reviews or Questions for ab184785.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com