Anti-Jagged1 抗体 [EPR26134-17] (BSA and Azide free) (ab300562)

This data was developed using 300561, the same antibody clone in a different buffer formulation. 

Blocking and diluting buffer and concentration: 5% NFDM/TBST. 

Negative control: Jurkat, MOLT-4 (PMID: 30231940).

200-kDa full length and 23-kDa C-terminal JAG1 are observed. The molecular weights are consistent with what has been described in the literature (PMID: 30890522, 30890522).

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

This data was developed using 300561, the same antibody clone in a different buffer formulation. 

Blocking and diluting buffer and concentration: 5% NFDM/TBST. 

200-kDa full length and 23-kDa C-terminal JAG1 are observed. The molecular weights are consistent with what has been described in the literature (PMID: 30890522, 30890522). 

Lane 3 of this blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

Exposure time: 

Lane 1: 59 seconds

Lane 2: 169 seconds

Lane 3: 3 minutes

This data was developed using ab300561, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Hepa1-6 (mouse hepatoma epithelial cells) labeling Jagged1 with ab300561 at 1/500 dilution (1.04 μg/ml), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 dilution (2 μg/ml) (Green). Confocal image showing cytoplasmic and membrane staining in Hepa1-6 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (2.5 μg/ml) (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: PBS was used instead of primary antobody, followed by preadsorbed secondary antibody ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (2 μg/ml).

This data was developed using ab300561, the same antibody clone in a different buffer formulation.

Jagged1 was immunoprecipitated from 0.35 mg Hepa1-6 (mouse hepatoma epithelial cell), 10 μg whole cell lysate with ab300561 at 1/30 dilution (2 μg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab300561 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Hepa1-6 (mouse hepatoma epithelial cell), whole cell lysate 10 ug

Lane 2: ab300561 IP in Hepa1-6 whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300561 in Hepa1-6 whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 7.75 seconds

200-kDa full length and 23-kDa C-terminal JAG1 are observed. The molecular weights are consistent with what has been described in the literature (PMID: 30890522, 30890522).