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AB211623

Anti-IZUMO1 抗体

Anti-IZUMO1 antibody

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(6 Publications)

Rabbit Polyclonal IZUMO1 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human IZUMO1 aa 200-300 conjugated to Keyhole Limpet Haemocyanin.

別名を表示する

Izumo sperm-egg fusion protein 1, Oocyte binding/fusion factor, Sperm-specific protein izumo, OBF, IZUMO1

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-IZUMO1 antibody (AB211623)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IZUMO1 antibody (AB211623)

Immunofluorescent analysis of human sperm labeling IZUMO1 using ab211623 at 1/100 dilution. An Alexa Fluor594 conjugated anti-rabbit IgG at 1/1000 dilution was used as secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IZUMO1 antibody (AB211623)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IZUMO1 antibody (AB211623)

Immunohistochemical analysis of formalin fixed and paraffin embedded mouse testis labeling IZUMO1 using ab211623 at 1/100 dilution followed by secondary detection usin an Alexafluor 488 goat anti rabbit (H&L) IgG at 1/1000 dilution (green). Nuclear counterstain : DAPI (blue).

Western blot - Anti-IZUMO1 antibody (AB211623)
  • WB

Supplier Data

Western blot - Anti-IZUMO1 antibody (AB211623)

10-20% SDS Page gradient gel.

All lanes:

Western blot - Anti-IZUMO1 antibody (ab211623) at 1/1000 dilution

All lanes:

mouse sperm lysate at 10 µg

Predicted band size: 39 kDa

false

Western blot - Anti-IZUMO1 antibody (AB211623)
  • WB

Supplier Data

Western blot - Anti-IZUMO1 antibody (AB211623)

All lanes:

Western blot - Anti-IZUMO1 antibody (ab211623) at 1/1000 dilution

All lanes:

human sperm lysate at 20 µg

Predicted band size: 39 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Human

アプリケーション

IHC-P, WB, ICC/IF

applications

免疫原

This product was produced with the following immunogens:

The exact immunogen used to generate this antibody is proprietary information.

Synthetic Peptide within Human IZUMO1 aa 300-350 conjugated to Keyhole Limpet Haemocyanin. Database link Q8IYV9

The exact immunogen used to generate this antibody is proprietary information.

Synthetic Peptide within Human IZUMO1 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. Database link Q8IYV9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/300", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製度
Whole antiserum
バッファー組成
pH: 6 - 8.5 Preservative: 0.1% Sodium azide
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

IZUMO1 also known as Immunoglobulin superfamily member 9 is a protein important to the process of fertilization. IZUMO1 has a molecular mass of approximately 37 kDa. It localizes specifically to the acrosomal region of sperm cells. This protein plays a mechanical role in sperm-egg fusion an essential step for successful fertilization allowing sperm cells to recognize and bind to the egg cell membrane. Expression of IZUMO1 is limited to mature sperm assuring its involvement exclusively during fertilization.
Biological function summary

The protein acts when spermatozoa interact with oocytes. IZUMO1 facilitates the binding through forming a complex with Juno the receptor on the egg surface. This interaction is necessary for the initial contact and fusion between the sperm and egg membranes. The IZUMO1-Juno pairing ensures proper docking and fusion enabling the combination of genetic materials from both gametes. The specificity of this binding prevents cross-species fertilization maintaining species integrity during reproduction.

Pathways

IZUMO1 has involvement in the fertilization pathway. It works directly in the sperm's fusion process an important part of the reproductive process. IZUMO1 interacts closely with other sperm proteins such as CD9 which play roles in adhesion and fusion events. These pathways incorporate IZUMO1 within the larger reproductive framework where it functions alongside these proteins to ensure successful gamete fusion and genetic transfer.

IZUMO1's functionality connects to issues of infertility. Defects or deficiencies in IZUMO1 expression or function can lead to male infertility due to ineffective sperm-egg fusion. Additionally fertility research identifies Juno as a complementary protein in this context as abnormalities in either protein may disrupt fertilization processes and contribute to reproductive challenges. Understanding IZUMO1 offers insight into diagnostic and therapeutic possibilities for treating infertility cases where sperm-egg membrane fusion is compromised.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Essential sperm cell-surface protein required for fertilization by acting as a ligand for IZUMO1R/JUNO receptor on egg. The IZUMO1 : IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'. Acts a ligand for the human-specific oolemma epitope FCRL3/MAIA during fertilization (PubMed : 36070373). FCRL3/MAIA replaces IZUMO1R/JUNO as IZUMO1 receptor after sperm-egg adhesion, which permits species-specific gamete fusion (PubMed : 36070373). Plays a critical role in sperm-oolemma binding prior to plasma membrane fusion. Can mediate cell-cell fusion in cultured mammalian cells independently of its binding to IZUMO1R/JUNO (By similarity).
See full target information IZUMO1
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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Aging cell 24:e70023 PubMed40248988

2025

Deciphering the Contribution of Circular RNAs to Age-Related Decline in Sertoli Cell Survivor.

Applications

Unspecified application

Species

Unspecified reactive species

Francesco Manfrevola,Nicola Mosca,Vincenza Grazia Mele,Teresa Chioccarelli,Guillaume Martinez,Charles Coutton,Monica Mattia,Mariaceleste Pezzullo,Silvia Fasano,Gilda Cobellis,Nicoletta Potenza,Rosanna Chianese

Human reproduction (Oxford, England) 39:1628-1644 PubMed38885964

2024

Variation of sperm quality and circular RNA content in men exposed to environmental contamination with heavy metals in 'Land of Fires', Italy.

Applications

Unspecified application

Species

Unspecified reactive species

Vincenza Grazia Mele,Teresa Chioccarelli,Nadia Diano,Donato Cappetta,Bruno Ferraro,Marialucia Telesca,Martina Moggio,Veronica Porreca,Antonella De Angelis,Liberato Berrino,Silvia Fasano,Gilda Cobellis,Rosanna Chianese,Francesco Manfrevola

Animals : an open access journal from MDPI 14: PubMed38612267

2024

Detection of Protein Biomarkers Relevant to Sperm Characteristics and Fertility in Semen in Three Wild Felidae: The Flat-Headed Cat (), Fishing Cat (), and Asiatic Golden Cat ().

Applications

Unspecified application

Species

Unspecified reactive species

Manita Wittayarat,Supalak Kiatsomboon,Navapol Kupthammasan,Wanlaya Tipkantha,Surasak Yimprasert,Ampika Thongphakdee,Saritvich Panyaboriban

International journal of biological sciences 19:2234-2255 PubMed37151878

2023

Cytochalasin D restores nuclear size acting on F-actin and IZUMO1 localization in low-quality spermatozoa.

Applications

Unspecified application

Species

Unspecified reactive species

Guillaume Martinez,Donato Cappetta,Marialucia Telesca,Konrad Urbanek,Giuseppe Castaldo,Magali Dhellemmes,Vincenza Grazia Mele,Teresa Chioccarelli,Veronica Porreca,Anne-Laure Barbotin,Angèle Boursier,Florian Guillou,Charles Coutton,Sophie Brouillet,Antonella De Angelis,Liberato Berrino,Riccardo Pierantoni,Gilda Cobellis,Rosanna Chianese,Francesco Manfrevola

International forum of allergy & rhinology 13:230-241 PubMed35950767

2022

Chronic interleukin-13 expression in mouse olfactory mucosa results in regional aneuronal epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Anirudh Saraswathula,Melissa M Liu,Heather Kulaga,Andrew P Lane

eLife 9: PubMed32484434

2020

TMEM95 is a sperm membrane protein essential for mammalian fertilization.

Applications

Unspecified application

Species

Unspecified reactive species

Ismael Lamas-Toranzo,Julieta G Hamze,Enrica Bianchi,Beatriz Fernández-Fuertes,Serafín Pérez-Cerezales,Ricardo Laguna-Barraza,Raúl Fernández-González,Pat Lonergan,Alfonso Gutiérrez-Adán,Gavin J Wright,María Jiménez-Movilla,Pablo Bermejo-Álvarez
View all publications
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代替製品

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AB211626

Anti-IZUMO1 antibody [125]

Immunocytochemistry/ Immunofluorescence - Anti-IZUMO1 antibody [125] (AB211626)

  • 0
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組合せ製品

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Primary Antibodies

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Primary Antibodies

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