Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
リコンビナントRabMAb

Anti-ITK/EMT 抗体 [Y402] (ab32507)

Submit a review Submit a question References (3)
Western blot - Anti-ITK/EMT antibody [Y402] (ab32507)
  • Immunoprecipitation - Anti-ITK/EMT antibody [Y402] (ab32507)
  • Western blot - Anti-ITK/EMT antibody [Y402] (ab32507)
  • Anti-ITK/EMT antibody [Y402] (ab32507)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y402] to ITK/EMT
  • Suitable for: WB, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-ITK/EMT antibody [Y402]
    ITK/EMT 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [Y402] to ITK/EMT

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IPmore details
    適用なし: Flow Cyt,ICC/IF or IHC

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Jurkat whole cell lysate (ab7899).

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.21% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    Y402

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab32507の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/10000. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).

For unpurified use at 1/1000.
IP
1/20.

For unpurified use at 1/50.
特記事項
WB
1/10000. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).

For unpurified use at 1/1000.
IP
1/20.

For unpurified use at 1/50.
追加情報
Is unsuitable for Flow Cyt,ICC/IF or IHC.

ターゲット情報

  • 機能

    Plays a role in T-cell proliferation and differentiation.

  • 組織特異性

    T-cell lines and natural killer cell lines.

  • 関連疾患

    Defects in ITK are the cause of lymphoproliferative syndrome EBV-associated autosomal type 1 (LPSA1) [MIM:613011]. LPSA1 is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Inadequate immune response to EBV can have a fatal outcome. Clinical features include splenomegaly, lymphadenopathy, anemia, thrombocytopenia, pancytopenia, recurrent infections. There is an increased risk for lymphoma.

  • 配列類似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
    Contains 1 Btk-type zinc finger.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.

  • 細胞内局在

    Cell membrane. Localizes to cell surface receptors in the plasma membrane after stimulation with respective receptors (TCR, CD28, CD2) in T-cells.
  • Information by UniProt

  • 参照データベース

    • 別名

      • EMT antibody
      • Homolog of mouse T cell itk/tsk antibody
      • IL 2 inducible T cell kinase antibody
      • IL2 inducible T cell kinase antibody
      • Interleukin 2 inducible T cell kinase antibody
      • Itk antibody
      • ITK_HUMAN antibody
      • Kinase EMT antibody
      • LPFS1 antibody
      • LYK antibody
      • MGC126257 antibody
      • MGC126258 antibody
      • PSCTK 2 antibody
      • PSCTK2 antibody
      • T cell specific kinase antibody
      • T-cell-specific kinase antibody
      • TSK antibody
      • Tyrosine protein kinase antibody
      • Tyrosine protein kinase ITK/TSK antibody
      • Tyrosine protein kinase Lyk antibody
      • Tyrosine-protein kinase ITK/TSK antibody
      • Tyrosine-protein kinase Lyk antibody
      see all

    画像

    • Western blot - Anti-ITK/EMT antibody [Y402] (ab32507)
      Western blot - Anti-ITK/EMT antibody [Y402] (ab32507)
      Lane 1 : Anti-ITK/EMT antibody [Y402] (ab32507) at 1/2000 dilution (purified)
      Lane 2 : Anti-ITK/EMT antibody [Y402] (ab32507) at 1/10000 dilution (purified)

      All lanes : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 72 kDa



      Blocking and diluting buffer: 5% NFDM/TBST

    • Immunoprecipitation - Anti-ITK/EMT antibody [Y402] (ab32507)
      Immunoprecipitation - Anti-ITK/EMT antibody [Y402] (ab32507)

      ab32507 (purified) at 1:20 dilution (1.0μg) immunoprecipitating ITK/EMT in Jurkat whole cell lysate.

      Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate, 10μg
      Lane 2 (+): ab32507 & Jurkat whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab172638 in Jurkat whole cell lysate

      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • Western blot - Anti-ITK/EMT antibody [Y402] (ab32507)
      Western blot - Anti-ITK/EMT antibody [Y402] (ab32507)
      Anti-ITK/EMT antibody [Y402] (ab32507) at 1/1000 dilution (unpurified) + Jurkat cell lysate

      Predicted band size: 72 kDa
      Observed band size: 72 kDa

    • Anti-ITK/EMT antibody [Y402] (ab32507)
      Anti-ITK/EMT antibody [Y402] (ab32507)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (3)

    ab32507 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab32507 は 3 報の論文で使用されています。

    • Patel V  et al. Comparison of Acalabrutinib, A Selective Bruton Tyrosine Kinase Inhibitor, with Ibrutinib in Chronic Lymphocytic Leukemia Cells. Clin Cancer Res 23:3734-3743 (2017). PubMed: 28034907
    • Reno TA  et al. Triptolide Inhibits Lung Cancer Cell Migration, Invasion, and Metastasis. Ann Thorac Surg 100:1817-24; discussion 1824-5 (2015). PubMed: 26298168
    • Huck K  et al. Girls homozygous for an IL-2-inducible T cell kinase mutation that leads to protein deficiency develop fatal EBV-associated lymphoproliferation. J Clin Invest 119:1350-8 (2009). PubMed: 19425169

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab32507.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com