Anti-ITK/EMT 抗体 [Y401] (ab32039)
![Flow Cytometry (Intracellular) - Anti-ITK/EMT antibody [Y401] (ab32039)](https://www.abcam.co.jp/ps/products/32/ab32039/Images/ab32039-506112-anti-itk-emt-antibody-y401-flowcytometry-jurkat-human.jpg "Flow Cytometry (Intracellular) - Anti-ITK/EMT antibody [Y401] (ab32039)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y401] to ITK/EMT
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-ITK/EMT antibody [Y401]
ITK/EMT 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [Y401] to ITK/EMT -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WBmore details
適用なし: IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human ITK/EMT aa 1-100 (N terminal). The exact sequence is proprietary.
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ポジティブ･コントロール
- WB: Jurkat whole cell lysate and MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate. Flow Cyt (Intra): Jurkat cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
Y401 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32039の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/1000 - 1/5000. Detects a band of approximately 68 kDa (predicted molecular weight: 72 kDa).
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| 特記事項 |
|---|
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Flow Cyt (Intra)
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
1/1000 - 1/5000. Detects a band of approximately 68 kDa (predicted molecular weight: 72 kDa). |
ターゲット情報
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機能
Plays a role in T-cell proliferation and differentiation. -
組織特異性
T-cell lines and natural killer cell lines. -
関連疾患
Defects in ITK are the cause of lymphoproliferative syndrome EBV-associated autosomal type 1 (LPSA1) [MIM:613011]. LPSA1 is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Inadequate immune response to EBV can have a fatal outcome. Clinical features include splenomegaly, lymphadenopathy, anemia, thrombocytopenia, pancytopenia, recurrent infections. There is an increased risk for lymphoma. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
Contains 1 Btk-type zinc finger.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
細胞内局在
Cell membrane. Localizes to cell surface receptors in the plasma membrane after stimulation with respective receptors (TCR, CD28, CD2) in T-cells. - Information by UniProt
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参照データベース
- Entrez Gene: 3702 Human
- Omim: 186973 Human
- SwissProt: Q08881 Human
- Unigene: 558348 Human
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別名
- EMT antibody
- Homolog of mouse T cell itk/tsk antibody
- IL 2 inducible T cell kinase antibody
see all
画像
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Flow Cytometry (Intracellular) - Anti-ITK/EMT antibody [Y401] (ab32039)
Intracellular Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling ITK/EMT with purified ab320396 at 1:50 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as a isotype control. Cell without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.
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![Western blot - Anti-ITK/EMT antibody [Y401] (ab32039)](https://www.abcam.co.jp/ps/products/32/ab32039/Images/ab32039-506111-anti-itk-emt-antibody-y401-westernblot-human.jpg)
Western blot - Anti-ITK/EMT antibody [Y401] (ab32039)All lanes : Anti-ITK/EMT antibody [Y401] (ab32039) at 1/1000 dilution
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lane 2 : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 72 kDa
Observed band size: 68 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
ab181602 was used as GAPDH loading control.
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![Anti-ITK/EMT antibody [Y401] (ab32039)](https://www.abcam.co.jp/ps/products/32/ab32039/Images/ab32039-4-benefits-of-recombinant-antibodies.png)
Anti-ITK/EMT antibody [Y401] (ab32039)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab32039 は 10 報の論文で使用されています。
- Ogishi M et al. Inherited human ITK deficiency impairs IFN-γ immunity and underlies tuberculosis. J Exp Med 220:N/A (2023). PubMed: 36326697
- Pan B et al. Interleukin-2 inducible T-cell kinase: a potential prognostic biomarker and tumor microenvironment remodeling indicator for hepatocellular carcinoma. Aging (Albany NY) 13:18620-18644 (2021). PubMed: 34282055
- Onidani K et al. Possible Therapeutic Strategy Involving the Purine Synthesis Pathway Regulated by ITK in Tongue Squamous Cell Carcinoma. Cancers (Basel) 13:N/A (2021). PubMed: 34283052
- Liu Y et al. ITK inhibition induced in vitro and in vivo anti-tumor activity through downregulating TCR signaling pathway in malignant T cell lymphoma. Cancer Cell Int 19:32 (2019). PubMed: 30814910
- Mamand S et al. Comparison of interleukin-2-inducible kinase (ITK) inhibitors and potential for combination therapies for T-cell lymphoma. Sci Rep 8:14216 (2018). PubMed: 30242208
- Miyata-Takata T et al. Expression of T-cell receptor signalling pathway components in extranodal NK/T-cell lymphoma. Histopathology 73:1030-1038 (2018). PubMed: 30102799
- Carson CC et al. IL2 Inducible T-cell Kinase, a Novel Therapeutic Target in Melanoma. Clin Cancer Res 21:2167-76 (2015). Human . PubMed: 25934889
- Schiralli Lester GM et al. Interleukin 2-inducible T cell kinase (ITK) facilitates efficient egress of HIV-1 by coordinating Gag distribution and actin organization. Virology 436:235-43 (2013). PubMed: 23260110
- Stepensky P et al. IL-2-inducible T-cell kinase deficiency: clinical presentation and therapeutic approach. Haematologica 96:472-6 (2011). WB ; Human . PubMed: 21109689
- Ramsay AG et al. Follicular lymphoma cells induce T-cell immunologic synapse dysfunction that can be repaired with lenalidomide: implications for the tumor microenvironment and immunotherapy. Blood 114:4713-20 (2009). PubMed: 19786615