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リコンビナントRabMAb

Anti-ITIH4 抗体 [EPR11233(2)] (ab180139)

Submit a review Submit a question References (4)
Western blot - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)
  • Immunoprecipitation - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)
  • Western blot - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)
  • Anti-ITIH4 antibody [EPR11233(2)] (ab180139)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11233(2)] to ITIH4
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-ITIH4 antibody [EPR11233(2)]
    ITIH4 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR11233(2)] to ITIH4

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IP, IHC-P, WBmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment within Human ITIH4 aa 1-150. The exact sequence is proprietary.
    Database link: Q14624

  • ポジティブ･コントロール

    • Human and mouse plasma, Mouse and rat serum, Human liver and placenta tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR11233(2)

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab180139の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP
1/60.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000 - 1/5000. Detects a band of approximately 120 kDa (predicted molecular weight: 103 kDa).
特記事項
IP
1/60.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000 - 1/5000. Detects a band of approximately 120 kDa (predicted molecular weight: 103 kDa).

ターゲット情報

  • 機能

    May be involved in acute phase reactions.

  • 組織特異性

    Liver specific.

  • 配列類似性

    Belongs to the ITIH family.
    Contains 1 VIT domain.
    Contains 1 VWFA domain.

  • 翻訳後修飾

    Cleaved by plasma kallikrein to yield 100 kDa and 35 kDa fragments, and the resulting 100 kDa fragment is further converted to a 70 kDa fragment.
    Appears to be both N- and O-glycosylated.
    The O-linked polysaccharides on Ser-696, Thr-701 and Ser-702 are probably the mucin type linked to GalNAc.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 35 kDa inter-alpha-trypsin inhibitor heavy chain H4 antibody
      • gp120 antibody
      • H4P antibody
      • IHRP antibody
      • Inter-alpha-inhibitor heavy chain 4 antibody
      • Inter-alpha-trypsin inhibitor family heavy chain-related protein antibody
      • Inter-alpha-trypsin inhibitor heavy chain H4 antibody
      • inter-alpha-trypsin inhibitor, heavy chain-like, 1 antibody
      • ITI heavy chain H4 antibody
      • ITI-HC4 antibody
      • ITIH4 antibody
      • ITIH4_HUMAN antibody
      • ITIHL1 antibody
      • OTTHUMP00000197120 antibody
      • OTTHUMP00000197121 antibody
      • OTTHUMP00000213834 antibody
      • OTTHUMP00000213869 antibody
      • PK-120 antibody
      • PK120 antibody
      • Plasma kallikrein sensitive glycoprotein 120 antibody
      • PRO1851 antibody
      see all

    画像

    • Western blot - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)
      Western blot - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)
      All lanes : Anti-ITIH4 antibody [EPR11233(2)] (ab180139) at 1/5000 dilution

      Lane 1 : Mouse serum
      Lane 2 : Mouse plasma
      Lane 3 : Rat serum

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1000 µg

      Predicted band size: 103 kDa
      Observed band size: 120 kDa why is the actual band size different from the predicted?



      Blocking buffer and concentration: 5%NFDM /TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)

      Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling ITIH4 with ab180139 at 1/500 dilution. Secondary antibody Goat anti rabbit IgG(HRP) prediluted. Counter stain Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)
      Immunoprecipitation - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)

      Western blot analysis of ITIH4 in immunoprecipitation pellets from Human plasma lysate. ab180139 used at 1/60 dilution for immunoprecipitation. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000. Blocking buffer and concentration: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ITIH4 with ab180139 at 1/500 dilution. Secondary antibody Goat anti rabbit IgG(HRP) prediluted. Counter stain Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Western blot - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)
      Western blot - Anti-ITIH4 antibody [EPR11233(2)] (ab180139)
      Anti-ITIH4 antibody [EPR11233(2)] (ab180139) at 1/5000 dilution + Human plasma at 10 µg

      Secondary
      Anti-Rabbit IgG (HRP) at 1/1000 dilution

      Predicted band size: 103 kDa
      Observed band size: 120 kDa why is the actual band size different from the predicted?



      Blocking buffer and concentration: 5%NFDM /TBST

    • Anti-ITIH4 antibody [EPR11233(2)] (ab180139)
      Anti-ITIH4 antibody [EPR11233(2)] (ab180139)

    参考文献 (4)

    ab180139 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab180139 は 4 報の論文で使用されています。

    • Yin R  et al. Proteomic landscape subtype and clinical prognosis of patients with the cognitive impairment by Japanese encephalitis infection. J Neuroinflammation 19:77 (2022). PubMed: 35379280
    • Pihl R  et al. ITIH4 acts as a protease inhibitor by a novel inhibitory mechanism. Sci Adv 7:N/A (2021). PubMed: 33523981
    • Jing D  et al. Effects of Helicobacter pylori infection and interleukin 6 on the expression of ITIH4 in human gastric cancer cells. Transl Cancer Res 9:4656-4665 (2020). PubMed: 35117829
    • Kumagai T  et al. Serum IgM Glycosylation Associated with Tuberculosis Infection in Mice. mSphere 4:N/A (2019). PubMed: 30918063

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