Anti-ITIH1/SHAP 抗体 [EPR17138-29] (ab204513)
![Western blot - Anti-ITIH1/SHAP antibody [EPR17138-29] (ab204513)](https://www.abcam.co.jp/ps/products/204/ab204513/Images/ab204513-248466-ab204513-wb-1.jpg "Western blot - Anti-ITIH1/SHAP antibody [EPR17138-29] (ab204513)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17138-29] to ITIH1/SHAP
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-ITIH1/SHAP antibody [EPR17138-29]
ITIH1/SHAP 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR17138-29] to ITIH1/SHAP -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Human fetal heart and fetal kidney lysates; Human brain, plasma and serum lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR17138-29 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab204513の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 80, 125, 250 kDa (predicted molecular weight: 101, 86 kDa).
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| 特記事項 |
|---|
|
WB
1/1000. Detects a band of approximately 80, 125, 250 kDa (predicted molecular weight: 101, 86 kDa). |
ターゲット情報
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機能
May act as a carrier of hyaluronan in serum or as a binding protein between hyaluronan and other matrix protein, including those on cell surfaces in tissues to regulate the localization, synthesis and degradation of hyaluronan which are essential to cells undergoing biological processes.
Contains a potential peptide which could stimulate a broad spectrum of phagocytotic cells. -
配列類似性
Belongs to the ITIH family.
Contains 1 VIT domain.
Contains 1 VWFA domain. -
翻訳後修飾
Heavy chains are linked to bikunin via chondroitin 4-sulfate esterified to the alpha-carboxyl of the C-terminal aspartate after propeptide cleavage.
The S-linked glycan is composed of two 6-carbon sugars, possibly Glc or Gal. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 3697 Human
- Omim: 147270 Human
- SwissProt: P19827 Human
- Unigene: 420257 Human
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別名
- H1P antibody
- IATIH antibody
- IGHEP1 antibody
see all
画像
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Western blot - Anti-ITIH1/SHAP antibody [EPR17138-29] (ab204513)All lanes : Anti-ITIH1/SHAP antibody [EPR17138-29] (ab204513) at 1/1000 dilution
Lane 1 : Human fetal heart tissue lysate
Lane 2 : Human fetal kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 101, 86 kDa
Observed band size: 125,250,80 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-ITIH1/SHAP antibody [EPR17138-29] (ab204513)](https://www.abcam.co.jp/ps/products/204/ab204513/Images/ab204513-248465-ab204513-wb-2.jpg)
Western blot - Anti-ITIH1/SHAP antibody [EPR17138-29] (ab204513)Anti-ITIH1/SHAP antibody [EPR17138-29] (ab204513) at 1/10000 dilution + Human brain lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 101, 86 kDa
Observed band size: 125,250,80 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-ITIH1/SHAP antibody [EPR17138-29] (ab204513)](https://www.abcam.co.jp/ps/products/204/ab204513/Images/ab204513-248464-ab204513-wb-3.jpg)
Western blot - Anti-ITIH1/SHAP antibody [EPR17138-29] (ab204513)Anti-ITIH1/SHAP antibody [EPR17138-29] (ab204513) at 1/20000 dilution + Human plasma lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 101, 86 kDa
Observed band size: 125,250,80 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-ITIH1/SHAP antibody [EPR17138-29] (ab204513)](https://www.abcam.co.jp/ps/products/204/ab204513/Images/ab204513-248463-ab204513-wb-4.jpg)
Western blot - Anti-ITIH1/SHAP antibody [EPR17138-29] (ab204513)Anti-ITIH1/SHAP antibody [EPR17138-29] (ab204513) at 1/50000 dilution + Human serum lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 101, 86 kDa
Observed band size: 125,250,80 kDa why is the actual band size different from the predicted?
Exposure time: 2 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Anti-ITIH1/SHAP antibody [EPR17138-29] (ab204513)](https://www.abcam.co.jp/ps/products/204/ab204513/Images/ab204513-6-benefits-of-recombinant-antibodies.png)
Anti-ITIH1/SHAP antibody [EPR17138-29] (ab204513)
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (1)
ab204513 は 1 報の論文で使用されています。
- Smith SM & Melrose J A Retrospective Analysis of the Cartilage Kunitz Protease Inhibitory Proteins Identifies These as Members of the Inter-a-Trypsin Inhibitor Superfamily with Potential Roles in the Protection of the Articulatory Surface. Int J Mol Sci 20:N/A (2019). PubMed: 30678366