Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-ITGA7 抗体 (ab75224)

Reviews (3)Q&A (4)References (5)
Western blot - Anti-ITGA7 antibody (ab75224)

    Key features and details

    • Rabbit polyclonal to ITGA7
    • Suitable for: ELISA, WB, IHC-Fr, IHC-P
    • Reacts with: Human, African green monkey
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-ITGA7 antibody
      ITGA7 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to ITGA7

    • 由来種

      Rabbit

    • 特異性

      ab75224 detects endogenous levels of a fragment of activated ITGA7 resulting from cleavage adjacent to Glu959.

    • アプリケーション

      適用あり: ELISA, WB, IHC-Fr, IHC-Pmore details

    • 種交差性

      交差種: Human, African green monkey

    • 免疫原

      Synthetic peptide derived from human ITGA7

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab75224の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    ELISA
    Use at an assay dependent concentration.
    WB (1)
    1/500 - 1/1000. Predicted molecular weight: 129 kDa. for the full length protein.
    IHC-Fr (1)
    Use at an assay dependent concentration.

    See Abreview.
    IHC-P
    Use at an assay dependent concentration.
    特記事項
    ELISA
    Use at an assay dependent concentration.
    WB
    1/500 - 1/1000. Predicted molecular weight: 129 kDa. for the full length protein.
    IHC-Fr
    Use at an assay dependent concentration.

    See Abreview.
    IHC-P
    Use at an assay dependent concentration.

    ターゲット情報

    • 機能

      Integrin alpha-7/beta-1 is the primary laminin receptor on skeletal myoblasts and adult myofibers. During myogenic differentiation, it may induce changes in the shape and mobility of myoblasts, and facilitate their localization at laminin-rich sites of secondary fiber formation. It is involved in the maintenance of the myofibers cytoarchitecture as well as for their anchorage, viability and functional integrity. Isoform Alpha-7X2B and isoform Alpha-7X1B promote myoblast migration on laminin 1 and laminin 2/4, but isoform Alpha-7X1B is less active on laminin 1 (In vitro). Acts as Schwann cell receptor for laminin-2. Acts as a receptor of COMP and mediates its effect on vascular smooth muscle cells (VSMCs) maturation (By similarity). Required to promote contractile phenotype acquisition in differentiated airway smooth muscle (ASM) cells.

    • 組織特異性

      Isoforms containing segment A are predominantly expressed in skeletal muscle. Isoforms containing segment B are abundantly expressed in skeletal muscle, moderately in cardiac muscle, small intestine, colon, ovary and prostate and weakly in lung and testes. Isoforms containing segment X2D are expressed at low levels in fetal and adult skeletal muscle and in cardiac muscle, but are not detected in myoblasts and myotubes. In muscle fibers isoforms containing segment A and B are expressed at myotendinous and neuromuscular junctions; isoforms containing segment C are expressed at neuromuscular junctions and at extrasynaptic sites. Isoforms containing segments X1 or X2 or, at low levels, X1X2 are expressed in fetal and adult skeletal muscle (myoblasts and myotubes) and cardiac muscle.

    • 関連疾患

      Defects in ITGA7 are the cause of muscular dystrophy congenital due to integrin alpha-7 deficiency (MDCI) [MIM:613204]. A form of congenital muscular dystrophy. Patients present at birth, or within the first few months of life, with hypotonia, muscle weakness and often with joint contractures.

    • 配列類似性

      Belongs to the integrin alpha chain family.
      Contains 7 FG-GAP repeats.

    • 発生段階

      In renewing intestinal epithelium, expression of isoforms containing segment B correlates with the onset of enterocytic differentiation.

    • 翻訳後修飾

      ADP-ribosylated on at least two sites of the extracellular domain in skeletal myotubes.
      A 70 kDa form is created by proteolytic cleavage. Cleavage is elevated during myogenic differentiation and the cleaved form enhances cell adhesion and spreading on laminin.

    • 細胞内局在

      Membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • alpha 7 antibody
        • FLJ25220 antibody
        • Integrin alpha 7 antibody
        • Integrin alpha 7 chain antibody
        • Integrin alpha 7 heavy chain antibody
        • Integrin alpha 7 light chain antibody
        • Integrin alpha-7 70 kDa form antibody
        • ITA7_HUMAN antibody
        • ITGA 7 antibody
        • Itga7 antibody
        see all

      画像

      • Western blot - Anti-ITGA7 antibody (ab75224)
        Western blot - Anti-ITGA7 antibody (ab75224)
        All lanes : Anti-ITGA7 antibody (ab75224) at 1/500 dilution

        Lane 1 : COS-7 cells,
        treated with etoposide (25 µM, 1 hour)
        Lane 2 : COS-7 cells,
        treated with etoposide (25 µM, 1 hour) with Cl-peptide at 5 µg

        Lysates/proteins at 5 µg per lane.

        Predicted band size: 129 kDa
        Additional bands at: 26 kDa (possible cleavage fragment)

      参考文献 (5)

      ab75224 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab75224 は 5 報の論文で使用されています。

      • Bugiardini E  et al. Integrin α7 Mutations Are Associated With Adult-Onset Cardiac Dysfunction in Humans and Mice. J Am Heart Assoc 11:e026494 (2022). PubMed: 36444867
      • Gwili N  et al. Transcriptome profiles of stem-like cells from primary breast cancers allow identification of ITGA7 as a predictive marker of chemotherapy response. Br J Cancer 125:983-993 (2021). PubMed: 34253873
      • Ahmed M  et al. Laminin a2 controls mouse and human stem cell behaviour during midbrain dopaminergic neuron development. Development 146:N/A (2019). PubMed: 31371375
      • Simon L  et al. Chronic binge alcohol consumption alters myogenic gene expression and reduces in vitro myogenic differentiation potential of myoblasts from rhesus macaques. Am J Physiol Regul Integr Comp Physiol 306:R837-44 (2014). PubMed: 24671243
      • Heller KN  et al. AAV-mediated overexpression of human a7 integrin leads to histological and functional improvement in dystrophic mice. Mol Ther 21:520-5 (2013). Human . PubMed: 23319059

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-7 of 7 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ITGA7 antibody

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Blocking step
      Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 22°C
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Tris
      Sample
      Human Tissue sections (Skeletal Muscle)
      Specification
      Skeletal Muscle
      Permeabilization
      No
      Fixative
      Methacarn
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Sep 27 2013

      Western blot abreview for Anti-ITGA7 antibody

       Average
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Tissue lysate - whole (Skeletal Muscle)
      Loading amount
      20 µg
      Specification
      Skeletal Muscle
      Gel Running Conditions
      Reduced Denaturing (12% Bis-Tris gel)
      Blocking step
      Li-Cor Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Jan 03 2013

      Immunohistochemistry (Frozen sections) abreview for Anti-ITGA7 antibody

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Human Tissue sections (Skeletal muscle)
      Specification
      Skeletal muscle
      Fixative
      none
      Permeabilization
      Yes - triton x-100
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      MS. Kristin Heller

      Verified customer

      投稿 Mar 25 2011

      Question

      We decided to replace the antibody with the Anti-ATPB antibody [3D5] - Mitochondrial Marker (ab14730).

      Read More
      Answer

      Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

      I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab14730. I kept the shipping address the same as you had requested.

      To check the status of the order please contact our Customer Service team and reference this number.

      Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

      I wish you the best of luck with your research.

      Read More

      Question

      we tested the antibody ITGA7 on human muscle (homogenate) by performing a WB analysis on a standard NuPage Novex 12% Bis Tris Gel and we found multiple bands. The bands were not around the predicted weight of 129 kDA (see attached image). Can you please help us? The protocol was a standard NuPage Novex Bis-Tris Gells protocol as described by the manufacturer.

      Read More
      Answer

      Thank you for contacting us.
      I am sorry to hear that the antibody does not give you the 129 kDa band.

      According to the Protein Database entry for human Integrin alpha 7 (http://www.uniprot.org/uniprot/Q13683), the protein is being cleaved into 3 chains: a heavy chain, a light chain and a 70 kDa form. The light chain has 3 glycosylation sites which can shift the MW of the light chain.

      Thus, I think the band you see at around 30 kDa is the light chain and the band at about 33 kDa is the gycosylated version.

      Also, you might want to try loading more protein (e.g. up to 50 ug) or use more primary antibody in order to obtain a band at 129 kDa - if the full length proteinis present in your samples.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

      Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

      Read More

      Question

      DESCRIPTION OF THE PROBLEM No signal or weak signal SAMPLE human muscle PRIMARY ANTIBODY 1/300 in 5% milk overnight at 4 degrees. 3 10min washes with .1%TBST DETECTION METHOD ECL Plus POSITIVE AND NEGATIVE CONTROLS USED Positive control is human muscle tissue- not even positive control isn't working ANTIBODY STORAGE CONDITIONS 4 degree SAMPLE PREPARATION SDS urea lysis buffer with Protease inhibitor, use sample reducing and w/LDS sample buffer heat for 10 mins to 70 degrees AMOUNT OF PROTEIN LOADED 20ug ELECTROPHORESIS/GEL CONDITIONS 3-8% nupage tris gel 150volts for 1hr TRANSFER AND BLOCKING CONDITIONS NuPage transfer buffer, 30v for 1 hr. block with 5% milk for 1 hr SECONDARY ANTIBODY Goat anti rabbit- 1:2000 for 1 hr. then 3 10min washes HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes ADDITIONAL NOTES it is working for IF

      Read More
      Answer

      Thank you for your response.

      Your protocol is sound however there are a few things that you may want to try in order to get this product to perform satisfactorily in western blot.
      I would consider using a different lysis buffer for your samples. The SDS/Urea buffer can be rather harsh. We suggest using an NP40 or a RIPA buffer for membrane bound proteins.
      Each isoform of this protein is over 100kDA. I would recommend boiling the samples at 95C for 10 minutes to ensure they are denatured.
      Do you see any bands above the expected area?
      If you are using PVDF? If so, you will need to activate the membrane by pre-wetting with methanol prior to transfer.
      Have you run loading controls?
      I look forward to your response and to hear how these suggestions work for you. Please let me know if you have any questions.

      Read More

      Question

      LOT NUMBER 843896 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM No signal or weak signal SAMPLE human muscle PRIMARY ANTIBODY 1/300 in 5% milk overnight at 4 degrees. 3 10min washes with .1%TBST DETECTION METHOD ECL Plus POSITIVE AND NEGATIVE CONTROLS USED Positive control is human muscle tissue- not even positive control isn't working ANTIBODY STORAGE CONDITIONS 4 degree SAMPLE PREPARATION SDS urea lysis buffer with Protease inhibitor, use sample reducing and w/LDS sample buffer heat for 10 mins to 70 degrees AMOUNT OF PROTEIN LOADED 20ug ELECTROPHORESIS/GEL CONDITIONS 3-8% nupage tris gel 150volts for 1hr TRANSFER AND BLOCKING CONDITIONS NuPage transfer buffer, 30v for 1 hr. block with 5% milk for 1 hr SECONDARY ANTIBODY Goat anti rabbit- 1:2000 for 1 hr. then 3 10min washes HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes ADDITIONAL NOTES it is working for IF

      Read More
      Answer

      Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

      The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

      Having reviewed the protocol details, I believe this product should have given satisfactory results. It appears that you may have received a faulty vial.


      I apologize for the inconvenience. If this product has been purchased in the last 6 months, I may offer you a free of charge replacement, credit note, or refund in compensation.



      Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com