Anti-Isoleucyl tRNA synthetase 抗体 (ab31533)
Key features and details
- Rabbit polyclonal to Isoleucyl tRNA synthetase
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Isoleucyl tRNA synthetase antibody
Isoleucyl tRNA synthetase 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Isoleucyl tRNA synthetase -
由来種
Rabbit -
特異性
This antibody reacts specifically with Isoleucyl tRNA synthetase protein. -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
S1074-F1264 denatured recombinant human IRS.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
Preservative: 0.05% Sodium azide -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab31533の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/2000. Predicted molecular weight: 145 kDa.
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| ICC/IF |
1/1000.
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| 特記事項 |
|---|
|
WB
1/2000. Predicted molecular weight: 145 kDa. |
|
ICC/IF
1/1000. |
ターゲット情報
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関連性
Isoleucyl tRNA synthetase is a component of multi ARS complex and catalyzes the ligation of isoleucine with isoleucyl tRNA. -
細胞内局在
Cytoplasmic -
参照データベース
- Entrez Gene: 3376 Human
- Omim: 600709 Human
- SwissProt: P41252 Human
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別名
- FLJ20736 antibody
- IARS antibody
- IARS1 antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Isoleucyl tRNA synthetase antibody (ab31533)ICC/IF image of ab31533 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31533, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-Isoleucyl tRNA synthetase antibody (ab31533)Anti-Isoleucyl tRNA synthetase antibody (ab31533) at 1/2000 dilution + whole cell lysate prepared from HEK293 cells at 50 µg/ml
Secondary
Goat anti-rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 145 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab31533 は 2 報の論文で使用されています。
- Jin D et al. HIV-1 Gag Binds the Multi-Aminoacyl-tRNA Synthetase Complex via the EPRS Subunit. Viruses 15:N/A (2023). PubMed: 36851687
- Baymiller M et al. Tissue-specific alternative splicing separates the catalytic and cell signaling functions of human leucyl-tRNA synthetase. J Biol Chem 298:101757 (2022). PubMed: 35202654