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リコンビナントRabMAb

Anti-ISL-2 抗体 [EPR12785] (ab176576)

Reviews (2) Submit a question
Western blot - Anti-ISL-2 antibody [EPR12785] (ab176576)
  • Flow Cytometry (Intracellular) - Anti-ISL-2 antibody [EPR12785] (ab176576)
  • Anti-ISL-2 antibody [EPR12785] (ab176576)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12785] to ISL-2
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-ISL-2 antibody [EPR12785]
    ISL-2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR12785] to ISL-2

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WBmore details
    適用なし: ICC/IF,IHC-P or IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Zebrafish非交差種: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human ISL-2 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q96A47

  • ポジティブ･コントロール

    • 293T, HeLa and HUVEC cell line lysates; 293 cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Tissue culture supernatant

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR12785

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab176576の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
追加情報
Is unsuitable for ICC/IF,IHC-P or IP.

ターゲット情報

  • 機能

    Transcriptional factor that defines subclasses of motoneurons that segregate into columns in the spinal cord and select distinct axon pathways.

  • 配列類似性

    Contains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • FLJ10160 antibody
      • Insulin gene enhancer protein ISL 2 antibody
      • Insulin gene enhancer protein ISL-2 antibody
      • Insulin gene enhancer protein ISL2 antibody
      • ISL 2 antibody
      • ISL 2 transcription factor antibody
      • ISL 2 transcription factor LIM/homeodomain antibody
      • ISL LIM homeobox 2 antibody
      • ISL2 antibody
      • ISL2 transcription factor antibody
      • ISL2 transcription factor LIM/homeodomain antibody
      • ISL2_HUMAN antibody
      • Islet 2 antibody
      • Islet-2 antibody
      • Islet2 antibody
      • SL2 transcription factor LIM/homeodomain (islet-2) antibody
      see all

    画像

    • Western blot - Anti-ISL-2 antibody [EPR12785] (ab176576)
      Western blot - Anti-ISL-2 antibody [EPR12785] (ab176576)
      All lanes : Anti-ISL-2 antibody [EPR12785] (ab176576) at 1/1000 dilution

      Lane 1 : HUVEC cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 40 kDa

    • Flow Cytometry (Intracellular) - Anti-ISL-2 antibody [EPR12785] (ab176576)
      Flow Cytometry (Intracellular) - Anti-ISL-2 antibody [EPR12785] (ab176576)

      Intracellular flow cytometric analysis of permeabilized 293 cells labeling Islet 2 with ab176576 at a 1/10 dilution (red), or a rabbit IgG negative control (green). 

       

       

    • Anti-ISL-2 antibody [EPR12785] (ab176576)
      Anti-ISL-2 antibody [EPR12785] (ab176576)

    参考文献 (0)

    ab176576 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab176576 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    IHC - Wholemount abreview for Anti-ISL-2 antibody [EPR12785]

     Average
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    IHC - Wholemount
    Sample
    Mouse Embryo (E12.5 whole embryo)
    Specification
    E12.5 whole embryo
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Alain Chedotal

    Verified customer

    投稿 Nov 25 2020

    Immunohistochemistry (Frozen sections) abreview for Anti-ISL-2 antibody [EPR12785]

     Average
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (E12.5 whole embryo)
    Permeabilization
    Yes - Triton
    Specification
    E12.5 whole embryo
    Blocking step
    Gelatin as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Alain Chedotal

    Verified customer

    投稿 Nov 25 2020

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