Anti-ISG20 抗体 (ab154393)
Key features and details
- Rabbit polyclonal to ISG20
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ISG20 antibody
ISG20 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ISG20 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Pig
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免疫原
Recombinant fragment corresponding to a region within amino acids 1-181 of Human ISG20 (Q96AZ6).
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ポジティブ･コントロール
- Raji whole cell lysate. HeLa cells. BT483 xenograft.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154393の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 20 kDa.
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| IHC-P | (1) |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 20 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Exonuclease with specificity for single-stranded RNA and, to a lesser extent for DNA. Degrades RNA at a rate that is approximately 35-fold higher than its rate for single-stranded DNA. Involved in the antiviral function of IFN against RNA viruses. -
組織特異性
Highly expressed in peripheral blood leukocytes, spleen, thymus, colon and lung. -
配列類似性
Belongs to the exonuclease superfamily. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 3669 Human
- Entrez Gene: 448855 Pig
- Omim: 604533 Human
- SwissProt: Q96AZ6 Human
- SwissProt: Q66UW5 Pig
- Unigene: 459265 Human
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別名
- CD25 antibody
- Estrogen regulated transcript 45 protein antibody
- Estrogen-regulated transcript 45 protein antibody
see all
画像
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Western blot - Anti-ISG20 antibody (ab154393)Anti-ISG20 antibody (ab154393) at 1/1000 dilution + Raji whole cell lysate at 30 µg
Predicted band size: 20 kDa
12% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISG20 antibody (ab154393)Image is courtesy of an AbReview submitted by Dr Assia Angelova.Immunohistochemical analysis of formaldehyde-fixed paraffin-embedded human non-Hodgkin lymphoma tissue sections, labelling ISG20 with ab154393 at a dilution of 1/50 incubated for 12 hours at 4°C. Heat mediated antigen retrival was with citrate buffer at pH 6.0 incubated for 15 minutes. Blocking was a with 5% serum incubated for 30 minutes at 25°C. Secondary was a Goat anti-rabbit polyclonal CY3® conjugate at 1/200. Counterstain was with DAPI against nuclear DNA.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISG20 antibody (ab154393)Immunohistochemical analysis of paraffin-embedded BT483 xenograft, labelling ISG20 with ab154393 at 1/500 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-ISG20 antibody (ab154393)Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labelling ISG20 with ab154393 at a 1/500 dilution. Co-stained with Hoechst 33342.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab154393 は 2 報の論文で使用されています。
- Stadler D et al. Interferon-induced degradation of the persistent hepatitis B virus cccDNA form depends on ISG20. EMBO Rep 22:e49568 (2021). PubMed: 33969602
- Zheng Z et al. Estradiol and proinflammatory cytokines stimulate ISG20 expression in synovial fibroblasts of patients with osteoarthritis. Intractable Rare Dis Res 6:269-273 (2017). PubMed: 29259855