AB154393

Anti-ISG20 抗体

Name: Anti-ISG20 抗体 (ab154393) | Abcam
Brand: Abcam Limited
SKU: AB154393
Price: 70000 JPY
Availability: InStock
Rating: 5 (1 reviews)

Anti-ISG20 antibody

(1 Review)

(3 Publications)

Rabbit Polyclonal ISG20 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ISG20 aa 1-200.

別名を表示する

HEM45, ISG20, Interferon-stimulated gene 20 kDa protein, Estrogen-regulated transcript 45 protein, Promyelocytic leukemia nuclear body-associated protein ISG20

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-ISG20 antibody (AB154393)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ISG20 antibody (AB154393)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labelling ISG20 with ab154393 at a 1/500 dilution. Co-stained with Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISG20 antibody (AB154393)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISG20 antibody (AB154393)

Immunohistochemical analysis of paraffin-embedded BT483 xenograft, labelling ISG20 with ab154393 at 1/500 dilution.

IHC-P

AbReview51270****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISG20 antibody (AB154393)

Immunohistochemical analysis of formaldehyde-fixed paraffin-embedded human non-Hodgkin lymphoma tissue sections, labelling ISG20 with ab154393 at a dilution of 1/50 incubated for 12 hours at 4°C. Heat mediated antigen retrival was with citrate buffer at pH 6.0 incubated for 15 minutes. Blocking was a with 5% serum incubated for 30 minutes at 25°C. Secondary was a Goat anti-rabbit polyclonal CY3^® conjugate at 1/200. Counterstain was with DAPI against nuclear DNA.

Image is courtesy of an AbReview submitted by Dr Assia Angelova.

Supplier Data

Western blot - Anti-ISG20 antibody (AB154393)

Samples were separated by 12% SDS-PAGE. The signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-ISG20 antibody (ab154393) at 1/1000 dilution

Lane 1:

Jurkat whole cell extracts at 30 µg

Lane 2:

Raji whole cell extracts at 30 µg

Lane 3:

NCI-H929 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 20 kDa

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Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

ICC/IF, WB, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human ISG20 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q96AZ6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

ISG20 also known as Interferon Stimulated Gene 20 kDa is an exonuclease enzyme with a molecular weight of around 20 kilodaltons. It degrades single-stranded RNA and DNA primarily involved in antiviral defense by targeting viral RNAs. ISG20 is widely expressed in several human tissues with high expression levels in immune-related cells and tissues such as lymph nodes spleen and blood leukocytes. This protein becomes strongly upregulated in response to interferons linking it to the body's innate immune response.

Biological function summary

ISG20 serves as an antiviral factor by degrading viral genomes to restrict infection. It does not work alone; often it functions as part of a larger antiviral mechanism alongside other interferon-induced proteins like PKR and OAS. Through its RNA-degrading activity ISG20 contributes significantly to controlling the spread of viruses by breaking down the RNA from invading organisms particularly RNA viruses. This makes ISG20 an important player in reducing viral loads during infections.

Pathways

ISG20 is mainly associated with the interferon signaling pathway where it acts downstream to execute immune processes. It interacts with and complements other antiviral proteins such as RNase L which also plays a role in degrading RNA during viral responses. Additionally ISG20 links to the broader regulation of apoptosis and cell death pathways showing how it assists in eliminating infected cells to prevent further viral proliferation.

ISG20 has implications in viral infections such as hepatitis C and influenza. Its role in degrading viral RNA positions it as a potential therapeutic target for mitigating these infections. During hepatitis C infection ISG20 works in conjunction with proteins like IRF9 to modulate immune response and limit virus replication. Researchers study ISG20 for its potential contribution to the severity of flu infections where its activity may influence the immune system's effectiveness in combating the influenza virus.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Interferon-induced antiviral exoribonuclease that acts mainly on single-stranded RNA (PubMed : 11401564, PubMed : 12594219, PubMed : 16033969). Exhibits antiviral activity against RNA viruses including hepatitis C virus (HCV), hepatitis A virus (HAV) and yellow fever virus (YFV) (PubMed : 16514659, PubMed : 21036379). Inhibition of several viruses such as chikungunya virus (CHIKV) does not involve the degradation of viral RNAs, but rather the inhibition of translation of viral proteins (By similarity). Exerts a translational control over a large panel of non-self RNA substrates while sparing endogenous transcripts. This activity correlates with the protein's ability to localize in cytoplasmic processing bodies (PubMed : 31600344). May also act as master regulator of over hundred interferon stimulated genes leading to viral genome translation inhibition (By similarity). May play additional roles in the maturation of snRNAs and rRNAs, and in ribosome biogenesis (PubMed : 16514659).

See full target information ISG20

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

EMBO reports 22:e49568 PubMed33969602

2021

Interferon-induced degradation of the persistent hepatitis B virus cccDNA form depends on ISG20.

Applications

Unspecified application

Species

Unspecified reactive species

Daniela Stadler,Martin Kächele,Alisha N Jones,Julia Hess,Christian Urban,Jessica Schneider,Yuchen Xia,Andreas Oswald,Firat Nebioglu,Romina Bester,Felix Lasitschka,Marc Ringelhan,Chunkyu Ko,Wen-Min Chou,Arie Geerlof,Maarten A van de Klundert,Jochen M Wettengel,Peter Schirmacher,Mathias Heikenwälder,Sabrina Schreiner,Ralf Bartenschlager,Andreas Pichlmair,Michael Sattler,Kristian Unger,Ulrike Protzer

Genes to cells : devoted to molecular & cellular mechanisms 26:18-30 PubMed33147372

2020

ISG20 and nuclear exosome promote destabilization of nascent transcripts for spliceosomal U snRNAs and U1 variants.

Applications

Unspecified application

Species

Unspecified reactive species

Takahito Kawamoto,Rei Yoshimoto,Ichiro Taniguchi,Makoto Kitabatake,Mutsuhito Ohno

Intractable & rare diseases research 6:269-273 PubMed29259855

2017

Estradiol and proinflammatory cytokines stimulate ISG20 expression in synovial fibroblasts of patients with osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiwei Zheng,Lin Wang,Jihong Pan

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ISG20 抗体

Anti-ISG20 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ISG20 antibody (AB154393)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISG20 antibody (AB154393)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISG20 antibody (AB154393)

Western blot - Anti-ISG20 antibody (AB154393)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (3)

代替製品

Primary Antibodies

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise