Anti-ISCU 抗体 (ab154060)
Key features and details
- Rabbit polyclonal to ISCU
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ISCU antibody
ISCU 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ISCU -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow
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免疫原
Recombinant fragment corresponding to a region within amino acids 1-167 of Human ISCU.
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ポジティブ･コントロール
- A549 and HeLa whole cell lysate (ab150035); HeLa cells; A549 xenograft tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154060の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 18 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 18 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Involved in the assembly or repair of the [Fe-S] clusters present in iron-sulfur proteins. Binds iron. -
組織特異性
Detected in heart, liver, skeletal muscle, brain, pancreas, kidney, lung and placenta. -
関連疾患
Defects in ISCU are the cause of myopathy with exercise intolerance Swedish type (MEIS) [MIM:255125]; also known as myopathy with deficiency of succinate dehydrogenase and aconitase or myoglobinuria due to abnormal glycolysis or hereditary myopathy with lactic acidosis (HML). This autosomal recessive metabolic disease is characterized by lifelong severe exercise intolerance, in which minor exertion causes fatigue of active muscles, shortness of breath, and cardiac palpitations in association with lactic acidosis. The biochemical phenotype is characterized by a deficiency in mitochondrial iron-sulfur proteins and impaired muscle oxidative metabolism. -
配列類似性
Belongs to the nifU family. -
細胞内局在
Cytoplasm. Nucleus and Mitochondrion. - Information by UniProt
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参照データベース
- Entrez Gene: 514789 Cow
- Entrez Gene: 23479 Human
- Entrez Gene: 66383 Mouse
- Entrez Gene: 288740 Rat
- Omim: 611911 Human
- SwissProt: Q9H1K1 Human
- SwissProt: Q9D7P6 Mouse
- Unigene: 615131 Human
see all -
別名
- 2310020H20Rik antibody
- HML antibody
- hnifU antibody
see all
画像
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Western blot - Anti-ISCU antibody (ab154060)Anti-ISCU antibody (ab154060) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 18 kDa
12% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-ISCU antibody (ab154060)Immunofluorescent analysis of methanol-fixed HeLa cells labeling ISCU with ab154060 at 1/200 dilution. Lower image co-stained with Hoechst 33342. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISCU antibody (ab154060)Immunohistochemical analysis of paraffin-embedded A549 xenograft tissue labeling ISCU with ab154060 at 1/500 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab154060 は論文での使用が確認できていません。