AB154060

Anti-ISCU 抗体

Name: Anti-ISCU 抗体 (ab154060) | Abcam
Brand: Abcam Limited
SKU: AB154060
Price: 74000 JPY
Availability: InStock

Anti-ISCU antibody

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(3 Publications)

Rabbit Polyclonal ISCU antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ISCU aa 1-200.

別名を表示する

NIFUN, ISCU, Iron-sulfur cluster assembly enzyme ISCU, NifU-like N-terminal domain-containing protein, NifU-like protein

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISCU antibody (AB154060)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISCU antibody (AB154060)

Immunohistochemical analysis of paraffin-embedded A549 xenograft tissue labeling ISCU with ab154060 at 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-ISCU antibody (AB154060)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ISCU antibody (AB154060)

Immunofluorescence analysis of HeLa cells fixed in ice-cold MeOH for 5 min labelling ISCU protein at mitochondria using ab154060 at a 1/500 dilution (Green). Hoechst 33342 was used for nuclear staining (Blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISCU antibody (AB154060)

Immunohistochemical analysis of mouse muscle tissue stained for ISCU protein at cytosol with ab154060 at a 1/500 dilution.

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Human

アプリケーション

ICC/IF, IHC-P, WB

applications

免疫原

Recombinant Fragment Protein within Human ISCU aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q9H1K1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)." }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)." }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

ISCU also known as the iron-sulfur cluster scaffold protein is an important player in the assembly of iron-sulfur clusters. This protein possesses a molecular mass of approximately 14 kDa and is widely expressed in mitochondria highlighting its central involvement in cellular processes dependent on these organelles. ISCU interacts with several proteins to facilitate the transfer of iron-sulfur clusters to apoproteins ensuring the proper maturation of these metalloproteins necessary for a variety of cellular functions.

Biological function summary

ISCU protein functions as an important scaffold component in the biosynthesis of iron-sulfur (Fe-S) clusters which are integral for the activity of multiple enzymatic and non-enzymatic systems. Within the mitochondria ISCU forms part of a multi-component complex that includes other scaffold proteins and necessary enzymes such as cysteine desulfurase which provides sulfur for cluster assembly. This complex orchestrates the precise coordination of metal ions and scaffold protein interactions facilitating cluster formation.

Pathways

The iron-sulfur cluster scaffold ISCU is vital in the electron transport chain and the citric acid cycle both of which are central to energy production in cells. ISCU closely interacts with proteins like frataxin which delivers iron to the scaffold and ferredoxin which is involved in electron transfer. These interactions highlight the pathway interdependence that maintains cellular bioenergetics and redox balance.

Disruptions in ISCU expression or function link to conditions such as Myopathy with exercise intolerance and Friedreich’s ataxia. Altered iron-sulfur cluster biogenesis impairs energy metabolism affecting muscle and neural tissues. ISCU's partnership with the frataxin protein becomes significant here as frataxin mutations result in similar pathological features thereby providing a potential target for therapeutic intervention in related diseases.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Isoform 1. Mitochondrial scaffold protein, of the core iron-sulfur cluster (ISC) assembly complex, that provides the structural architecture on which the [2Fe-2S] clusters are assembled (PubMed : 34824239). The core iron-sulfur cluster (ISC) assembly complex is involved in the de novo synthesis of a [2Fe-2S] cluster, the first step of the mitochondrial iron-sulfur protein biogenesis. This process is initiated by the cysteine desulfurase complex (NFS1 : LYRM4 : NDUFAB1) that produces persulfide which is delivered on the scaffold protein ISCU in a FXN-dependent manner. Then this complex is stabilized by FDX2 which provides reducing equivalents to accomplish the [2Fe-2S] cluster assembly. Finally, the [2Fe-2S] cluster is transferred from ISCU to chaperone proteins, including HSCB, HSPA9 and GLRX5 (Probable) (PubMed : 24971490, PubMed : 29576242, PubMed : 30031876, PubMed : 34824239). Exists as two slow interchanging conformational states, a structured (S) and disordered (D) form (PubMed : 23940031). May modulate NFS1 desulfurase activity in a zinc-dependent manner (PubMed : 30031876). Modulates the interaction between FXN and the cysteine desulfurase complex (PubMed : 29576242).. Isoform 2. Cytoplasmic scaffold protein, of the cytoplasmic core iron-sulfur cluster (ISC) assembly complex that provides the structural architecture on which the Fe-S clusters are assembled and may be involved in the cytoplasmic iron-sulfur protein biogenesis.

See full target information ISCU

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

PLoS genetics 21:e1011597 PubMed39908322

2025

Plant-specific cochaperone SSR1 affects root elongation by modulating the mitochondrial iron-sulfur cluster assembly machinery.

Applications

Unspecified application

Species

Unspecified reactive species

Xuanjun Feng,Yue Hu,Tao Xie,Huiling Han,Diana Bonea,Lijuan Zeng,Jie Liu,Wenhan Ying,Bona Mu,Yuanyuan Cai,Min Zhang,Yanli Lu,Rongmin Zhao,Xuejun Hua

Anticancer research 44:4709-4721 PubMed39477289

2024

Genome-wide microRNA Analysis Identified Over-expression in Pancreatic Cancer Tissues as a Predictor of their Local Invasiveness.

Applications

Unspecified application

Species

Unspecified reactive species

Tomohisa Otsu,Masamichi Hayashi,Keizo Fujita,Daigo Kobayashi,Nobuhiko Nakagawa,Keisuke Kurimoto,Hideki Takami,Koki Nakanishi,Shinichi Umeda,Dai Shimizu,Norifumi Hattori,Mitsuro Kanda,Chie Tanaka,Goro Nakayama,Yasuhiro Kodera

Aging 12:25229-25255 PubMed33223504

2020

Exposure to ozone impacted Th1/Th2 imbalance of CD T cells and apoptosis of ASMCs underlying asthmatic progression by activating lncRNA PVT1-miR-15a-5p/miR-29c-3p signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yangyang Wei,Baofen Han,Wenjuan Dai,Shufang Guo,Caiping Zhang,Lixuan Zhao,Yan Gao,Yi Jiang,Xiaomei Kong

View all publications

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Abcam product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ISCU 抗体

Anti-ISCU antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISCU antibody (AB154060)

Immunocytochemistry/ Immunofluorescence - Anti-ISCU antibody (AB154060)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ISCU antibody (AB154060)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

代替製品

Primary Antibodies

組合せ製品

Primary Antibodies

Primary Antibodies

Reagents, Controls & Accessories

Primary Antibodies

Reactivity data

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (3)

Abcam product promise