Anti-IRX7 抗体 (ab234984)
Key features and details
- Rabbit polyclonal to IRX7
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-IRX7 antibody -
製品の詳細
Rabbit polyclonal to IRX7 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Rat, Human -
免疫原
Recombinant fragment corresponding to Human IRX7 aa 250-400.
Database link: P78412 -
ポジティブ･コントロール
- WB: A549 whole cell lysate; Rat heart lysate. IHC-P: Human cervical cancer tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Protein G purified -
特記事項（精製）
Purity >95%. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab234984の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/200 - 1/500.
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| WB |
1/500 - 1/5000. Predicted molecular weight: 48 kDa.
|
| 特記事項 |
|---|
|
IHC-P
1/200 - 1/500. |
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WB
1/500 - 1/5000. Predicted molecular weight: 48 kDa. |
ターゲット情報
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配列類似性
Belongs to the TALE/IRO homeobox family.
Contains 1 homeobox DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 79190 Human
- Entrez Gene: 307715 Rat
- Omim: 606196 Human
- SwissProt: P78412 Human
- Unigene: 369907 Human
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別名
- Homeodomain protein IRXB3 antibody
- Iroquois class Homeodomain Protein 6 antibody
- Iroquois class homeodomain protein IRX 6 antibody
see all
画像
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Western blot - Anti-IRX7 antibody (ab234984)All lanes : Anti-IRX7 antibody (ab234984) at 1/500 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : Rat heart lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 48 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRX7 antibody (ab234984)Paraffin-embedded human cervical cancer tissue stained for IRX7 using ab234984 at 1/200 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30minutes at room temperature. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab234984 は論文での使用が確認できていません。