Anti-IRS4 抗体 [EP907Y] (ab52622)
![Western blot - Anti-IRS4 antibody [EP907Y] (ab52622)](https://www.abcam.co.jp/ps/products/52/ab52622/Images/ab52622-450781-anti-irs4-antibody-ep907y-westernblot-hek-293-human.jpg "Western blot - Anti-IRS4 antibody [EP907Y] (ab52622)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP907Y] to IRS4
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-IRS4 antibody [EP907Y]
IRS4 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP907Y] to IRS4 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details
適用なし: Flow Cyt or IHC-P -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: HEK-293 whole cell lysate; IP: HEK-293 whole cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP907Y -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab52622の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/10000. Detects a band of approximately 133 kDa (predicted molecular weight: 133 kDa).
For unpurified use at 1/5000 dilution |
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| IP |
1/20.
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| 特記事項 |
|---|
|
WB
1/10000. Detects a band of approximately 133 kDa (predicted molecular weight: 133 kDa). For unpurified use at 1/5000 dilution |
|
IP
1/20. |
ターゲット情報
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関連性
IRS4 (Insulin receptor substrate 4) is a cytoplasmic protein that contains many potential tyrosine and serine/threonine phosphorylation sites. Tyrosine-phosphorylated IRS4 protein has been shown to associate with cytoplasmic signaling molecules that contain SH2 domains. The IRS4 protein is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation. -
細胞内局在
Cell membrane; Peripheral membrane protein; Cytoplasmic side. -
参照データベース
- Entrez Gene: 8471 Human
- Omim: 603510 Human
- SwissProt: O14654 Human
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別名
- 160 kDa phosphotyrosine protein antibody
- Insulin receptor substrate 4 antibody
- IRS 4 antibody
- PY160 antibody
画像
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Western blot - Anti-IRS4 antibody [EP907Y] (ab52622)Anti-IRS4 antibody [EP907Y] (ab52622) at 1/10000 dilution (Purified) + HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 133 kDa
Observed band size: 133 kDaBlocking buffer: 5% NFDM/TBST
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![Immunoprecipitation - Anti-IRS4 antibody [EP907Y] (ab52622)](https://www.abcam.co.jp/ps/products/52/ab52622/Images/ab52622-4-anti-irs4-antibody-ep907y-immunoprecipitation-hek293-human.jpg)
Immunoprecipitation - Anti-IRS4 antibody [EP907Y] (ab52622)Purified ab52622 at 1/20 dilution (0.5µg) immunoprecipitating IRS4 in HEK-293 whole cell lysate.
Lane 1 (input): HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab52622 + HEK-293 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52622 in HEK-293 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 133 kDa
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab52622 は 8 報の論文で使用されています。
- Yang F et al. Long Noncoding RNA LINC00173 Promotes the Malignancy of Melanoma by Promoting the Expression of IRS4 Through Competitive Binding to microRNA-493. Cancer Manag Res 12:3131-3144 (2020). PubMed: 32440211
- Li X et al. Phosphorylation of IRS4 by CK1?2 promotes its degradation by CHIP through the ubiquitin/lysosome pathway. Theranostics 8:3643-3653 (2018). PubMed: 30026872
- Weischenfeldt J et al. Pan-cancer analysis of somatic copy-number alterations implicates IRS4 and IGF2 in enhancer hijacking. Nat Genet 49:65-74 (2017). PubMed: 27869826
- Cui A et al. Downregulation of miR-493 promoted melanoma proliferation by suppressing IRS4 expression. Tumour Biol 39:1010428317701640 (2017). PubMed: 28475006
- Homma Y et al. Insulin receptor substrate-4 binds to Slingshot-1 phosphatase and promotes cofilin dephosphorylation. J Biol Chem 289:26302-13 (2014). PubMed: 25100728
- Chai B et al. A novel transcript is up-regulated by fasting in the hypothalamus and enhances insulin signaling. J Neuroendocrinol : (2012). PubMed: 22935015
- Mertens F et al. The t(X;6) in subungual exostosis results in transcriptional deregulation of the gene for insulin receptor substrate 4. Int J Cancer 128:487-91 (2011). PubMed: 20340132
- Karrman K et al. The t(X;7)(q22;q34) in paediatric T-cell acute lymphoblastic leukaemia results in overexpression of the insulin receptor substrate 4 gene through illegitimate recombination with the T-cell receptor beta locus. Br J Haematol 144:546-51 (2009). WB ; Human . PubMed: 19055661