Anti-IRS1 (phospho Y632) 抗体 [EPR259(2)] (ab109543)
![Western blot - Anti-IRS1 (phospho Y632) antibody [EPR259(2)] (ab109543)](https://www.abcam.co.jp/ps/products/109/ab109543/Images/ab109543-122493-anti-irs1-phospho-y632-antibody-epr2592-western-blot.jpg "Western blot - Anti-IRS1 (phospho Y632) antibody [EPR259(2)] (ab109543)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR259(2)] to IRS1 (phospho Y632)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-IRS1 (phospho Y632) antibody [EPR259(2)]
IRS1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR259(2)] to IRS1 (phospho Y632) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- 293T cell lysate
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR259(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109543の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 132 kDa.
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| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 132 kDa. |
追加情報
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
ターゲット情報
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機能
May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit. -
関連疾患
Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]. -
配列類似性
Contains 1 IRS-type PTB domain.
Contains 1 PH domain. -
翻訳後修飾
Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
Phosphorylation of Tyr-896 is required for GRB2-binding. - Information by UniProt
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参照データベース
- Entrez Gene: 3667 Human
- Omim: 147545 Human
- SwissProt: P35568 Human
- Unigene: 471508 Human
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別名
- HIRS 1 antibody
- HIRS1 antibody
- Insulin receptor substrate 1 antibody
see all
画像
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Western blot - Anti-IRS1 (phospho Y632) antibody [EPR259(2)] (ab109543)All lanes : Anti-IRS1 (phospho Y632) antibody [EPR259(2)] (ab109543) at 1/1000 dilution
Lane 1 : 293T cell lysate, untreated
Lane 2 : 293T cell lysate treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 132 kDa -
![Anti-IRS1 (phospho Y632) antibody [EPR259(2)] (ab109543)](https://www.abcam.co.jp/ps/products/109/ab109543/Images/ab109543-2-benefits-of-recombinant-antibodies.png)
Anti-IRS1 (phospho Y632) antibody [EPR259(2)] (ab109543)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab109543 は 5 報の論文で使用されています。
- Li H et al. Dendrobium officinale polysaccharide decreases podocyte injury in diabetic nephropathy by regulating IRS-1/AKT signal and promoting mitophagy. Aging (Albany NY) 15:10291-10306 (2023). PubMed: 37812195
- Martínez Báez A et al. The Insulin Receptor Substrate 2 Mediates the Action of Insulin on HeLa Cell Migration via the PI3K/Akt Signaling Pathway. Curr Issues Mol Biol 45:2296-2308 (2023). PubMed: 36975518
- Zong J et al. Bromodomain-containing protein 2 promotes lipolysis via ERK/HSL signalling pathway in white adipose tissue of mice. Gen Comp Endocrinol 281:105-116 (2019). PubMed: 31121164
- Zhang J et al. Establishment of a novel hepatic steatosis cell model by Cas9/sgRNA-mediated DGK? gene knockout. Mol Med Rep 17:2169-2176 (2018). PubMed: 29207074
- Song Q et al. Concomitant inhibition of receptor tyrosine kinases and downstream AKT synergistically inhibited growth of KRAS/BRAF mutant colorectal cancer cells. Oncotarget 8:5003-5015 (2017). PubMed: 28002807