Anti-IRS1 (phospho S639) 抗体 (ab47404)
Key features and details
- Rabbit polyclonal to IRS1 (phospho S639)
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-IRS1 (phospho S639) antibody
IRS1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IRS1 (phospho S639) -
由来種
Rabbit -
特異性
This antibody detects endogenous levels of human IRS-1 only when phosphorylated at serine 639. In rat and mouse the phosphorilation site in at serine 635. -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human IRS1 aa 600-700 (phospho S639).
Database link: P35568 -
ポジティブ･コントロール
- Human breast carcinoma tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using a non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab47404の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit. -
関連疾患
Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]. -
配列類似性
Contains 1 IRS-type PTB domain.
Contains 1 PH domain. -
翻訳後修飾
Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
Phosphorylation of Tyr-896 is required for GRB2-binding. - Information by UniProt
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参照データベース
- Entrez Gene: 3667 Human
- Entrez Gene: 16367 Mouse
- Entrez Gene: 25467 Rat
- Omim: 147545 Human
- SwissProt: P35568 Human
- SwissProt: P35569 Mouse
- SwissProt: P35570 Rat
- Unigene: 471508 Human
see all -
別名
- HIRS 1 antibody
- HIRS1 antibody
- Insulin receptor substrate 1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRS1 (phospho S639) antibody (ab47404)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IRS-1 (phospho-Ser639) antibody in the presence and absence of blocking peptide. (antibody dilution: 1/50)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab47404 は 1 報の論文で使用されています。
- Pircher A et al. Antidiabetic drugs influence molecular mechanisms in prostate cancer. Cancer Biol Ther 19:1153-1161 (2018). PubMed: 30067448