Anti-IRS1 抗体 [EP260Y] (ab46800)
![Western blot - Anti-IRS1 antibody [EP260Y] (ab46800)](https://www.abcam.co.jp/ps/products/46/ab46800/Images/ab46800-496881-anti-irs1-antibody-ep260y-western-blot-human.jpg "Western blot - Anti-IRS1 antibody [EP260Y] (ab46800)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP260Y] to IRS1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-IRS1 antibody [EP260Y]
IRS1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP260Y] to IRS1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: His-tagged human IRS1 recombinant protein (aa801-1020)
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特記事項
This antibody was re-validated and was confirmed to recognize total IRS1. The name was changed from Anti-IRS1 (phospho Y896) antibody [EP260Y] to Anti-IRS1 antibody [EP260Y] on May 1st, 2018.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP260Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab46800の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/10000. Predicted molecular weight: 27 kDa.
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| 特記事項 |
|---|
|
WB
1/10000. Predicted molecular weight: 27 kDa. |
ターゲット情報
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機能
May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit. -
関連疾患
Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]. -
配列類似性
Contains 1 IRS-type PTB domain.
Contains 1 PH domain. -
翻訳後修飾
Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
Phosphorylation of Tyr-896 is required for GRB2-binding. - Information by UniProt
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参照データベース
- Entrez Gene: 3667 Human
- Omim: 147545 Human
- SwissProt: P35568 Human
- Unigene: 471508 Human
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別名
- HIRS 1 antibody
- HIRS1 antibody
- Insulin receptor substrate 1 antibody
see all
画像
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Western blot - Anti-IRS1 antibody [EP260Y] (ab46800)Anti-IRS1 antibody [EP260Y] (ab46800) at 1/10000 dilution + His-tagged human IRS1 recombinant protein (aa801-1020), 10 ng
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 27 kDa
Exposure time: 1 secondBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Primary and secondary antibodies incubated for 1hr at room temperature.
Predicted band size of IRS1 recombinant protein fragment is 27 kDa.
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![Anti-IRS1 antibody [EP260Y] (ab46800)](https://www.abcam.co.jp/ps/products/46/ab46800/Images/ab46800-3-benefits-of-recombinant-antibodies.png)
Anti-IRS1 antibody [EP260Y] (ab46800)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (17)
ab46800 は 17 報の論文で使用されています。
- Deng X et al. Cimifugin inhibits adipogenesis and TNF-α-induced insulin resistance in 3T3-L1 cells. Open Med (Wars) 18:20230855 (2023). PubMed: 38045856
- Zhao J et al. CircCCDC91 regulates chicken skeletal muscle development by sponging miR-15 family via activating IGF1-PI3K/AKT signaling pathway. Poult Sci 101:101803 (2022). PubMed: 35334442
- Peng L et al. Amelioration of Hippocampal Insulin Resistance Reduces Tau Hyperphosphorylation and Cognitive Decline Induced by Isoflurane in Mice. Front Aging Neurosci 13:686506 (2021). PubMed: 34512303
- Li L et al. Mesenchymal stromal cells protect hepatocytes from lipotoxicity through alleviation of endoplasmic reticulum stress by restoring SERCA activity. J Cell Mol Med 25:2976-2993 (2021). PubMed: 33591626
- Rivera P et al. Perinatal free-choice of a high-calorie low-protein diet affects leptin signaling through IRS1 and AMPK dephosphorylation in the hypothalami of female rat offspring in adulthood. Acta Physiol (Oxf) 226:e13244 (2019). PubMed: 30589509