Anti-IRR 抗体 [EP327Y] (ab40782)
![Western blot - Anti-IRR antibody [EP327Y] (ab40782)](https://www.abcam.co.jp/ps/products/40/ab40782/Images/ab40782-53953-anti-irr-antibody-ep327y-western-blot.jpg "Western blot - Anti-IRR antibody [EP327Y] (ab40782)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP327Y] to IRR
- Suitable for: WB, IHC-P
- Reacts with: Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-IRR antibody [EP327Y]
IRR 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP327Y] to IRR -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Rat, Human
交差が予測される動物種: Mouse
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免疫原
Synthetic peptide within Human IRR aa 1-100 (N terminal). The exact sequence is proprietary.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP327Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab40782の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/2000. Predicted molecular weight: 80, 144 kDa.
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
1/1000 - 1/2000. Predicted molecular weight: 80, 144 kDa. |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
This receptor probably binds an insulin related protein and has a tyrosine-protein kinase activity. It phosphorylates the insulin receptor substrates IRS-1 and IRS-2. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 3 fibronectin type-III domains.
Contains 1 protein kinase domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3645 Human
- Entrez Gene: 23920 Mouse
- Entrez Gene: 60663 Rat
- Omim: 147671 Human
- SwissProt: P14616 Human
- SwissProt: Q9WTL4 Mouse
- SwissProt: Q64716 Rat
- Unigene: 248138 Human
see all -
別名
- INSRR antibody
- INSRR_HUMAN antibody
- Insulin Receptor R antibody
see all
画像
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Western blot - Anti-IRR antibody [EP327Y] (ab40782)All lanes : Anti-IRR antibody [EP327Y] (ab40782) at 1/2000 dilution
Lane 1 : PC12 cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : SHSY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 80, 144 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Additional bands at: 130 kDa, 37 kDa. We are unsure as to the identity of these extra bands. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRR antibody [EP327Y] (ab40782)](https://www.abcam.co.jp/ps/products/40/ab40782/Images/ab40782-53952-anti-irr-antibody-ep327y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRR antibody [EP327Y] (ab40782)Immunohistochemical analysis of paraffin-embedded human kidney tissue using ab40782.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-IRR antibody [EP327Y] (ab40782)](https://www.abcam.co.jp/ps/products/40/ab40782/Images/ab40782-3-benefits-of-recombinant-antibodies.png)
Anti-IRR antibody [EP327Y] (ab40782)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab40782 は 3 報の論文で使用されています。
- Liu H et al. Magnesium supplementation enhances insulin sensitivity and decreases insulin resistance in diabetic rats. Iran J Basic Med Sci 23:990-998 (2020). PubMed: 32952944
- Wang L et al. Exosomes derived from pancreatic cancer cells induce insulin resistance in C2C12 myotube cells through the PI3K/Akt/FoxO1 pathway. Sci Rep 7:5384 (2017). PubMed: 28710412
- Li Y et al. LRP4 mutations alter Wnt/beta-catenin signaling and cause limb and kidney malformations in Cenani-Lenz syndrome. Am J Hum Genet 86:696-706 (2010). WB ; Human . PubMed: 20381006