Anti-Iroquois homeobox protein 3/IRX3 抗体 [EPR12124] (ab174307)
![Western blot - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR12124] (ab174307)](https://www.abcam.co.jp/ps/products/174/ab174307/Images/ab174307-199193-anti-iroquois-homeobox-protein-3-antibody-epr12124-western-blot.jpg "Western blot - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR12124] (ab174307)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12124] to Iroquois homeobox protein 3/IRX3
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR12124]
Iroquois homeobox protein 3/IRX3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR12124] to Iroquois homeobox protein 3/IRX3 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WBmore details
適用なし: ICC/IF,IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide within Human Iroquois homeobox protein 3/IRX3 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: P78415 -
ポジティブ･コントロール
- Hela, Jurkat, A431 and K562 cell lysates; K562 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR12124 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab174307の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
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| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 52 kDa. |
追加情報
Is unsuitable for ICC/IF,IHC-P or IP.
ターゲット情報
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機能
Transcription factor. Involved in SHH-dependent neural patterning. Together with NKX2-2 and NKX6-1 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class I proteins of neuronal progenitor factors, which are repressed by SHH signals. Involved in the transcriptional repression of MNX1 in non-motor neuron cells. -
配列類似性
Belongs to the TALE/IRO homeobox family.
Contains 1 homeobox DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 79191 Human
- Entrez Gene: 16373 Mouse
- Entrez Gene: 307721 Rat
- Omim: 612985 Human
- SwissProt: P78415 Human
- SwissProt: P81067 Mouse
- Unigene: 499205 Human
- Unigene: 238044 Mouse
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別名
- AI894186 antibody
- Homeodomain protein IRXB1 antibody
- Iroquois class homeodomain protein IRX 1 antibody
see all
画像
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Western blot - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR12124] (ab174307)All lanes : Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR12124] (ab174307) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : A431 cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 52 kDa -
![Flow Cytometry (Intracellular) - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR12124] (ab174307)](https://www.abcam.co.jp/ps/products/174/ab174307/Images/ab174307-199194-anti-iroquois-homeobox-protein-3-antibody-epr12124-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR12124] (ab174307)Intracellular flow cytometric analysis of permeabilized K562 cellslabeling Iroquois homeobox protein 3/IRX3 with ab174307 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
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![Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR12124] (ab174307)](https://www.abcam.co.jp/ps/products/174/ab174307/Images/ab174307-3-benefits-of-recombinant-antibodies.png)
Anti-Iroquois homeobox protein 3/IRX3 antibody [EPR12124] (ab174307)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab174307 は 4 報の論文で使用されています。
- Zhang Z et al. IRX3 Overexpression Enhances Ucp1 Expression In Vivo. Front Endocrinol (Lausanne) 12:634191 (2021). PubMed: 33776928
- Singh B et al. Important Role of FTO in the Survival of Rare Panresistant Triple-Negative Inflammatory Breast Cancer Cells Facing a Severe Metabolic Challenge. PLoS One 11:e0159072 (2016). WB ; Human . PubMed: 27390851
- Landgraf K et al. FTO Obesity Risk Variants Are Linked to Adipocyte IRX3 Expression and BMI of Children - Relevance of FTO Variants to Defend Body Weight in Lean Children? PLoS One 11:e0161739 (2016). WB . PubMed: 27560134
- Huang JZ et al. Catalpol preserves neural function and attenuates the pathology of Alzheimer's disease in mice. Mol Med Rep 13:491-6 (2016). PubMed: 26531891