Anti-IRGM 抗体 (ab118569)
Key features and details
- Rabbit polyclonal to IRGM
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-IRGM antibody
IRGM 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IRGM -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide corresponding to 14 amino acids from near the centre of Human IRGM.
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ポジティブ･コントロール
- Human Colon and Brain tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted. -
バッファー
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab118569の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 20 kDa.
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| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 20 kDa. |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Putative GTPase which is required for clearance of acute protozoan and bacterial infections. Functions in innate immune response probably through regulation of autophagy. May regulate proinflammatory cytokine production and prevent endotoxemia upon infection. May also play a role in macrophages adhesion and motility. -
組織特異性
Widely expressed (at protein level). Expressed in several tissues including colon, small bowel and peripheral blood leukocytes. -
関連疾患
Defects in IRGM are the cause of susceptibility to inflammatory bowel disease type 19 (IBD19) [MIM:612278]. A chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints. -
配列類似性
Belongs to the interferon-inducible GTPase family. -
細胞内局在
Golgi apparatus membrane. Cell membrane. Cytoplasmic vesicle > phagosome membrane. Cytoplasmic vesicle > autophagosome membrane. Cell projection > phagocytic cup. Behaves like an integral membrane protein (By similarity). Recruited to the plasma membrane around forming phagocytic cups, it remains associated with maturing autophagosomes (By similarity). Preferentially associated with cis- and medial-Golgi. - Information by UniProt
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参照データベース
- Entrez Gene: 345611 Human
- Entrez Gene: 15944 Mouse
- Entrez Gene: 303090 Rat
- SwissProt: A1A4Y4 Human
- SwissProt: Q60766 Mouse
- SwissProt: Q6AYC2 Rat
- Unigene: 519680 Human
- Unigene: 652148 Human
see all -
別名
- IFI1 antibody
- Iigp3 antibody
- Iipg3 antibody
see all
画像
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Western blot - Anti-IRGM antibody (ab118569)All lanes : Anti-IRGM antibody (ab118569) at 2 µg/ml
Lane 1 : Wild-type A549 cell lysate
Lane 2 : IRGM knockout A549 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?Western blot: Anti-IRGM antibody (ab118569) staining at 2 ug/ml, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab118569 was shown to bind specifically to IRGM. A band was observed at 18 kDa in wild-type A549 cell lysates with no signal observed at this size in IRGM knockout cell line. To generate this image, wild-type and IRGM knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRGM antibody (ab118569)Human Colon (formalin-fixed, paraffin-embedded) stained with IRGM antibody ab118569 at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. -
Western blot - Anti-IRGM antibody (ab118569)Lane 1 : Anti-IRGM antibody (ab118569) at 1 µg/ml
Lane 2 : Anti-IRGM antibody (ab118569) at 2 µg/ml
All lanes : Human Brain lysate
Predicted band size: 20 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab118569 は 2 報の論文で使用されています。
- Cozzolino F et al. New label-free methods for protein relative quantification applied to the investigation of an animal model of Huntington Disease. PLoS One 15:e0238037 (2020). PubMed: 32886703
- Gobert AP et al. Hypusination Orchestrates the Antimicrobial Response of Macrophages. Cell Rep 33:108510 (2020). PubMed: 33326776