Anti-IRF8 抗体 (ab245607)
Key features and details
- Rabbit polyclonal to IRF8
- Suitable for: IP, WB
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-IRF8 antibody
IRF8 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IRF8 -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Mouse -
免疫原
Synthetic peptide within Mouse IRF8 aa 374-424. The exact sequence is proprietary. NP_032346.1
Database link: P23611 -
ポジティブ･コントロール
- WB: RAW 264.7 and L1210 whole cell lysate. IP: IRF8 IP in RAW 264.7 whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab245607 was affinity purified using an epitope specific to IRF8 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab245607の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at 2-5 µg/mg of lysate.
|
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| WB |
1/2000 - 1/10000.
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| 特記事項 |
|---|
|
IP
Use at 2-5 µg/mg of lysate. |
|
WB
1/2000 - 1/10000. |
ターゲット情報
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機能
Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Plays a negative regulatory role in cells of the immune system. -
組織特異性
Predominantly in lymphoid tissues. -
配列類似性
Belongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain. -
翻訳後修飾
Ubiquitinated. Ubiquitination by TRIM21 in macrophages, a process that is strongly increased upon interferon gamma stimulation, leds to the enhanced transcriptional activity of target cytokine genes. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 15900 Mouse
- SwissProt: P23611 Mouse
- Unigene: 334861 Mouse
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別名
- H ICSBP antibody
- H-ICSBP antibody
- HGNC:5358 antibody
see all
画像
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Western blot - Anti-IRF8 antibody (ab245607)Anti-IRF8 antibody (ab245607) at 0.1 µg/ml + L1210 whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 30 secondsPrepared using NETN lysis buffer.
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Western blot - Anti-IRF8 antibody (ab245607)All lanes : Anti-IRF8 antibody (ab245607) at 0.1 µg/ml
Lane 1 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 50 µg
Lane 2 : RAW 264.7 whole cell lysate at 15 µg
Developed using the ECL technique.
Exposure time: 3 minutesPrepared using NETN lysis buffer.
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Immunoprecipitation - Anti-IRF8 antibody (ab245607)IRF8 was immunoprecipitated from RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab245607 used for IP at 3 µg per reaction. For WB 1 µg/ml.Lane 1: ab245607 IP in RAW 264.7 whole cell lysate.
Lane 2: Control IgG IP in RAW 264.7 whole cell lysate.
Chemiluminescence detection: 3 minutes.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab245607 は 1 報の論文で使用されています。
- Boto P et al. Zbtb46-dependent altered developmental program in embryonic stem cell-derived blood cell progenitors. Stem Cells N/A:N/A (2021). PubMed: 34058047