Anti-IRF1 抗体 (ab245338)
Key features and details
- Rabbit polyclonal to IRF1
- Suitable for: WB, IP
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-IRF1 antibody
IRF1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IRF1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Mouse -
免疫原
Synthetic peptide within Mouse IRF1 aa 200-250. The exact sequence is proprietary. (NP_032416.1).
Database link: P15314 -
ポジティブ･コントロール
- WB: NIH/3T3 whole cell lysate. IP: NIH/3T3 whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab245338 was affinity purified using an epitope specific to IRF1 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab245338の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 37 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| 特記事項 |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 37 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
ターゲット情報
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機能
Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and activates those genes. Acts as a tumor suppressor. -
関連疾患
Defects in IRF1 are a cause of gastric cancer (GASC) [MIM:613659]; also called gastric cancer intestinal or stomach cancer. Gastric cancer is a malignant disease which starts in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. The term gastric cancer or gastric carcinoma refers to adenocarcinoma of the stomach that accounts for most of all gastric malignant tumors. Two main histologic types are recognized, diffuse type and intestinal type carcinomas. Diffuse tumors are poorly differentiated infiltrating lesions, resulting in thickening of the stomach. In contrast, intestinal tumors are usually exophytic, often ulcerating, and associated with intestinal metaplasia of the stomach, most often observed in sporadic disease. -
配列類似性
Belongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain. -
翻訳後修飾
Sumoylation represses the transcriptional activity and displays enhanced resistance to protein degradation. Inactivates the tumor suppressor activity. Elevated levels in tumor cells. Major site is Lys-275. Sumoylation is enhanced by PIAS3 (By similarity). Desumoylated by SENP1 in tumor cells and appears to compete with ubiquitination on C-terminal sites.
Ubiquitinated. Appears to compete with sumoylation on C-terminal sites. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 16362 Mouse
- SwissProt: P15314 Mouse
- Unigene: 105218 Mouse
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別名
- Interferon regulatory factor 1 antibody
- Interferon regulatory factor 1 isoform +I9 antibody
- Interferon regulatory factor 1 isoform d78 antibody
see all
画像
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Western blot - Anti-IRF1 antibody (ab245338)All lanes : Anti-IRF1 antibody (ab245338) at 0.2 µg/ml
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Lane 2 : NIH/3T3 whole cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 37 kDa
Exposure time: 1 minute -
Immunoprecipitation - Anti-IRF1 antibody (ab245338)IRF1 was immunoprecipitated from NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245338 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245338 at 1 µg/ml.
Lane 1: ab245338 IP in NIH/3T3 whole cell lysate.
Lane 2: Control IgG IP in NIH/3T3 whole cell lysate.Detection: Chemiluminescence with exposure time of 60 seconds.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab245338 は論文での使用が確認できていません。