Anti-IREB2/IRP2 抗体 (ab106926)
Key features and details
- Goat polyclonal to IREB2/IRP2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-IREB2/IRP2 antibody
IREB2/IRP2 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to IREB2/IRP2 -
由来種
Goat -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Pig, Orangutan
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免疫原
Synthetic peptide corresponding to Human IREB2/IRP2 aa 489-503 (internal sequence).
Sequence:C-SIHYEGSEYKLSHGS
Database link: NP_004127.1 -
ポジティブ･コントロール
- WB: Human Liver lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab106926 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab106926の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use a concentration of 3 - 6 µg/ml.
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| WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 105 kDa).
1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
|
IHC-P
Use a concentration of 3 - 6 µg/ml. |
|
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 105 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA. -
配列類似性
Belongs to the aconitase/IPM isomerase family. -
翻訳後修飾
Ubiquitinated and degraded by the proteasome in presence of high level of iron and oxygen. Ubiquitinated by a SCF complex containing FBXL5. Upon iron and oxygen depletion FBXL5 is degraded, preventing ubiquitination and allowing its RNA-binding activity. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 539404 Cow
- Entrez Gene: 3658 Human
- Entrez Gene: 64602 Mouse
- Entrez Gene: 100174315 Orangutan
- Entrez Gene: 100153993 Pig
- Entrez Gene: 64831 Rat
- Omim: 147582 Human
- SwissProt: P48200 Human
see all -
別名
- ACO3 antibody
- FLJ23381 antibody
- IRE BP2 antibody
see all
画像
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Western blot - Anti-IREB2/IRP2 antibody (ab106926)All lanes : Anti-IREB2/IRP2 antibody (ab106926) at 0.3 µg/ml
Lane 1 : Human Liver lysate in RIPA buffer
Lane 2 : Human Liver lysate in RIPA buffer with immunizing peptide
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 105 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.
Primary incubation was 1 hour.
An additional band of unknown identity was consistently observed at 60kDa. This band was successfully blocked by incubation with the immunizing peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab106926 は 1 報の論文で使用されています。
- Cloonan SM et al. Mitochondrial iron chelation ameliorates cigarette smoke-induced bronchitis and emphysema in mice. Nat Med 22:163-74 (2016). PubMed: 26752519