AB124945

Anti-IRE1 (phospho S724) 抗体 [EPR5253]

Name: Anti-IRE1 (phospho S724) 抗体 [EPR5253] (ab124945) | Abcam
Brand: Abcam Limited
SKU: AB124945
Price: 40000 JPY
Availability: InStock
Rating: 4 (8 reviews)

Anti-IRE1 (phospho S724) antibody [EPR5253]

RabMAb
Recombinant
詳細を見る

(8 Reviews)

(149 Publications)

Anti-IRE1 (phospho S724) antibody [EPR5253] (ab124945) is a rabbit monoclonal antibody detecting IRE1 in Western Blot, Dot Blot. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency
- Over 110 publications

別名を表示する

IRE1, ERN1, Serine/threonine-protein kinase/endoribonuclease IRE1, Endoplasmic reticulum-to-nucleus signaling 1, Inositol-requiring protein 1, Ire1-alpha, hIRE1p, IRE1a

3 Images

Lab

Western blot - Anti-IRE1 (phospho S724) antibody [EPR5253] (AB124945)

Blocking/Diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-IRE1 (phospho S724) antibody [EPR5253] (ab124945) at 1/200 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) treated with 1uM Thapsigargin for 1 hour whole cell lysates at 20 µg

Lane 3:

HeLa (Human cervix adenocarcinoma epithelial cell) treated with 1uM Thapsigargin for 6 hours whole cell lysates at 20 µg

Lane 4:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates at 20 µg

Lane 5:

HEK-293 (Human embryonic kidney epithelial cell) treated with 10mM DTT for 1 hour whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 110 kDa

Observed band size: 110 kDa

false

Exposure time: 180s

OI-RD Scanning - Anti-IRE1 (phospho S724) antibody [EPR5253] (AB124945)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-IRE1 (phospho S724) antibody [EPR5253] (AB124945)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Lab

Dot Blot - Anti-IRE1 (phospho S724) antibody [EPR5253] (AB124945)

Dot Blot analysis of Lane 1 : IRE1 (pS724) phospho peptide and Lane 2 : IRE1 non-phospho peptide labeling IRE1 (phospho S724) with ab124945 at 1/1000 dilution. 5% NFDM/TBST was used as the diluting and blocking buffer. ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100000 dilution. Exposure time : 3 minutes.

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR5253

アイソタイプ

IgG

キャリアフリー

交差種

Human

アプリケーション

WB, Dot

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/1000", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Synthetic peptide": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

製品の詳細

What is this antibody validated in?
Anti-IRE1 (phospho S724) antibody [EPR5253] (ab124945) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Dot Blot in Human samples.

What is the molecular weight of IRE1?
Anti-IRE1 (phospho S724) [EPR5253] (ab124945) specifically detects a band for IRE1 (UniProt: O75460) at a molecular weight of 110kDa.

Trusted by the scientific community
Anti-IRE1 (phospho S724) [EPR5253] (ab124945) was first used in a scientific publication in 2012 and has been cited over 110 times in peer-reviewed journals.

Reviewed by scientists
Anti-IRE1 (phospho S724) [EPR5253] (ab124945) has over 5 independent reviews from customers.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR5253] also available for your convenience: ab124945, Carrier free - ab240009

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Stable for 12 months at -20°C

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The inositol-requiring enzyme 1 (IRE1) also known as ERN1 or IRE1 alpha is a critical endoplasmic reticulum (ER) stress sensor. It has a molecular weight of approximately 110 kDa. IRE1 is expressed in various cell types and tissues particularly in those subject to a high degree of protein synthesis such as the liver pancreas and secretory cells. This protein plays a dual role as both a RNase and a kinase which enables it to respond swiftly to misfolded proteins accumulating in the ER.

Biological function summary

IRE1 is an important regulator in the unfolded protein response (UPR) a cellular reaction to stress in the ER. It operates as part of a complex mechanism facilitating the splicing of X-box binding protein 1 (XBP1) mRNA which results in the production of a potent transcription factor. IRE1 activity helps in restoring normal function of the cell by upregulating genes involved in protein folding secretion and degradation. Its actions are important for maintaining cellular homeostasis during stressful conditions.

Pathways

IRE1 is an integral component of the UPR pathway which works to alleviate ER stress. It interacts closely with other UPR transducers such as activating transcription factor 6 (ATF6) and protein kinase RNA-like ER kinase (PERK). IRE1 connects with the XBP1 pathway facilitating adaptive responses that enhance protein-folding capacity lipid biosynthesis and ER-associated degradation. Altogether these pathways mediate cell survival or apoptosis depending on the severity of the stress.

IRE1 has significant involvement in conditions like diabetes and cancer. In the context of diabetes improper UPR signaling due to chronic ER stress leads to insulin resistance and pancreatic beta-cell dysfunction. In cancer IRE1 modulates tumor microenvironment and promotes cancer cell survival under hypoxic conditions. The XBP1 pathway linked with IRE1 also plays a substantial role in these diseases by influencing cell proliferation and apoptosis. Understanding the mechanisms of IRE1 in these conditions might provide therapeutic insights.

style

left-column

style

left-column

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR) (PubMed : 11175748, PubMed : 11779464, PubMed : 12637535, PubMed : 21317875, PubMed : 28128204, PubMed : 30118681, PubMed : 9637683). In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP (PubMed : 21317875). Accumulation of misfolded proteins in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity (PubMed : 21317875). The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA (PubMed : 11779464, PubMed : 21317875, PubMed : 24508390). The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes (PubMed : 11779464, PubMed : 21317875, PubMed : 24508390). Acts as an upstream signal for ER stress-induced GORASP2-mediated unconventional (ER/Golgi-independent) trafficking of CFTR to cell membrane by modulating the expression and localization of SEC16A (PubMed : 21884936, PubMed : 28067262).

See full target information ERN1 phospho S724

style

left-column

文献 (149)

Recent publications for all applications. Explore the full list and refine your search

European journal of medical research 30:859 PubMed41013641

2025

RHBDD1 induces cell cycle arrest in TP53-mutant NSCLC cells by promoting endoplasmic reticulum-associated degradation of p53 and DNA-PKcs.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Chen,Yang Wang,Shuai Shen,Libin Zhang,Jun Liu,Xiangyun Yan,Hao Peng,Zheyuan Xu

iScience 28:113121 PubMed40792035

2025

Manganese suppresses tumor growth through hyper-activating IRE1α.

Applications

Unspecified application

Species

Unspecified reactive species

Ruoxi Shi,Ming Wang,Si Chen,Xinwei Hu,Xudong Zhao,Shixin Zhou,Congli Fan,Zihan Zhou,Likun Wang

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 39:e70739 PubMed40542649

2025

Mitochondria-Homing Drug Mitochonic Acid 5 Improves Barth Syndrome Myopathy in a Human-Induced Pluripotent Stem Cell Model and Barth Syndrome Drosophila Model.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshiyasu Tongu,Tomoko Kasahara,Tetsuro Matsuhashi,Yoshitsugu Oikawa,Ryota Akimoto,Yuhan Luo,Sayaka Sekine,Momoka Suzuki,Hitomi Kashiwagi,Shinichiro Kanno,Yoshikazu Tanaka,Kyohei Sato,Yusuke Okubo,Akihiko Muto,Hidetaka Tokuno,Chitose Suzuki,Chiharu Kawabe,Takamasa Ishikawa,Shun Watanabe,Koichi Kikuchi,Shun Itai,Takeya Sato,Takehiro Suzuki,Kazuhiro Igarashi,Shinji Fukuda,Tomoyoshi Soga,Kei Murayama,Erina Kuranaga,Takafumi Toyohara,Takaaki Abe

Stem cells international 2025:5091529 PubMed40476182

2025

miR-1275 Delivered via Mesenchymal Stem Cell-Derived Extracellular Vesicles Regulates ER-Phagy Through AXIN2 in Nucleus Pulposus Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiwu Dong,Hailong Zhang,Wenwei Yang,Keliang Huang,Xin Zhang,Lianxiang Xing,Ying Zhang,Kewen Zhao

Viruses 17: PubMed40143290

2025

Modulation of Ire1-Xbp1 Defense Pathway in Encephalomyocarditis Virus-Infected HeLa Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Shishova,Yury Ivin,Ekaterina Gladneva,Ksenia Fominykh,Ilya Dyugay,Anatoly Gmyl

Scientific reports 15:5943 PubMed39966508

2025

ERS regulates endometrial epithelial cell autophagy through XBP1s-mediated activation of the PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kangkang Gao,Yiteng Zhao,Mengqi Si,Beibei Zhang,Zongjie Wang,Huatao Chen,Pengfei Lin,Aihua Wang,Yaping Jin

Frontiers in pharmacology 15:1462421 PubMed39949397

2025

FAM134B-mediated endoplasmic reticulum autophagy protects against cisplatin-induced spiral ganglion neuron damage.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Wang,Yue Xu,Yajie Wang,Qian Liu,Yanan Li,Weiwei Zhang,Huiming Nong,Junhong Zhang,Hao Zhao,Huaqian Yang,Lingchuan Guo,Jianfeng Li,Hong Li,Qianqian Yang

Lipids in health and disease 24:44 PubMed39934848

2025

Arachidonic acid is involved in high-salt diet-induced coronary remodeling through stimulation of the IRE1α/XBP1s/RUNX2/OPN signaling cascade.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuoran Jia,Jian Wu,Fang Liu,Huimin Wang,Peiyang Zheng,Bing Shen,Ren Zhao

Cell communication and signaling : CCS 23:72 PubMed39930412

2025

Transcription factor XBP1s promotes endometritis-induced epithelial-mesenchymal transition by targeting MAP3K2, a key gene in the MAPK/ERK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Kangkang Gao,Mengqi Si,Xinxi Qin,Beibei Zhang,Zongjie Wang,Pengfei Lin,Huatao Chen,Aihua Wang,Yaping Jin

Clinical and experimental medicine 25:15 PubMed39708203

2024

FUS and METTL3 collaborate to regulate RNA maturation, preventing unfolded protein response and promoting gastric cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Dongtao Liu,Bo Ding,Gang Liu,Zhijuan Yang

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IRE1 (phospho S724) 抗体 [EPR5253]

Anti-IRE1 (phospho S724) antibody [EPR5253]

Western blot - Anti-IRE1 (phospho S724) antibody [EPR5253] (AB124945)

OI-RD Scanning - Anti-IRE1 (phospho S724) antibody [EPR5253] (AB124945)

Dot Blot - Anti-IRE1 (phospho S724) antibody [EPR5253] (AB124945)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (149)

代替製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

組合せ製品

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Abcam product promise