Anti-IRE1 (phospho S724) 抗体 (ab48187)
Key features and details
- Rabbit polyclonal to IRE1 (phospho S724)
- Suitable for: WB, ELISA, IHC-P
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-IRE1 (phospho S724) antibody
IRE1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IRE1 (phospho S724) -
由来種
Rabbit -
アプリケーション
適用あり: WB, ELISA, IHC-Pmore details -
種交差性
交差種: Mouse, Human, Recombinant fragment -
免疫原
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ポジティブ･コントロール
- WB: HeLa, Min6, transfected COS-7 cells. IHC-P: Human spleen tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.025% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab48187の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (14) |
1/1000 - 1/2000. Predicted molecular weight: 110 kDa.
Block with 3-5% BSA. |
| ELISA |
1/100 - 1/2000.
|
|
| IHC-P | (1) |
1/300.
|
| 特記事項 |
|---|
|
WB
1/1000 - 1/2000. Predicted molecular weight: 110 kDa. Block with 3-5% BSA. |
|
ELISA
1/100 - 1/2000. |
|
IHC-P
1/300. |
ターゲット情報
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機能
Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis. -
組織特異性
Ubiquitously expressed. High levels observed in pancreatic tissue. -
配列類似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 KEN domain.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated. -
細胞内局在
Endoplasmic reticulum membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 10595 Human
- Entrez Gene: 2081 Human
- Entrez Gene: 78943 Mouse
- Omim: 604033 Human
- SwissProt: O75460 Human
- SwissProt: Q76MJ5 Human
- SwissProt: Q9EQY0 Mouse
- SwissProt: Q9Z2E3 Mouse
see all -
別名
- Endoplasmic reticulum (ER) to nucleus signalling 1 antibody
- Endoplasmic reticulum to nucleus signaling 1 antibody
- Endoplasmic reticulum-to-nucleus signaling 1 antibody
see all
画像
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Western blot - Anti-IRE1 (phospho S724) antibody (ab48187)All lanes : Anti-IRE1 (phospho S724) antibody (ab48187) at 1/2000 dilution
Lane 1 : HeLa cells 30 nM Calyculin A: AP-buffer
Lane 2 : HeLa cells 30 nM Calyculin A: Alkaline Phosphatase
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : infrared (IR)-labelled goat anti-rabbit (green) antibody and IR-labelled goat anti-mouse (red) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 110 kDaThe blots were produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membranes were blocked for an hour. Membrane 2 was incubated with alkaline phosphatase (AP; 100 U per mL) for one hour, whilst membrane 1 was treated with AP-buffer only, before being incubated with ab48187 (rabbit anti-IRE1 antibody diluted 1:2000) and loading control ab125247 (mouse anti-GAPDH antibody; diluted 1:10,000) for 24 hours at 4°C. Antibody binding was detected using infrared (IR)-labelled goat anti-rabbit (green) antibody and IR-labelled goat anti-mouse (red) at 1:10,000 dilutions for 1 hour at room temperature before imaging.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRE1 (phospho S724) antibody (ab48187)Paraffin-embedded human spleen tissue stained for IRE1 using ab48187 at 1/300 dilution for 1 hour at room temperature in immunohistochemical analysis. DAB staining. Counter stained with hematoxylin.
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ELISA - Anti-IRE1 (phospho S724) antibody (ab48187)Serially diluted ab48187 was bound to immobilised Phospho peptide (133861) - or Control peptide (1 microgram x mL-1). The antibody was detected by HRP-labelled goat anti-rabbit IgG (ab97080; diluted 50000 times) and signal was developed with TMB substrate.
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Western blot - Anti-IRE1 (phospho S724) antibody (ab48187)All lanes : Anti-IRE1 (phospho S724) antibody (ab48187) at 1/1000 dilution
Lane 1 : Min6 cells untreated
Lane 2 : Min6 cells treated with glucose for 3 hours at 5 mM
Lane 3 : Min6 cells treated with glucose for 3 hours at 20 mM
Developed using the ECL technique.
Predicted band size: 110 kDa -
Western blot - Anti-IRE1 (phospho S724) antibody (ab48187)All lanes : Anti-IRE1 (phospho S724) antibody (ab48187) at 1/2000 dilution
Lane 1 : Cell lysate prepared from COS-7 Untransfected cells
Lane 2 : Cell lysate prepared from COS-7 cells expressing wild type IRE1 alpha
Lane 3 : Cell lysate prepared from COS-7 cells expressing kinase-dead IRE1 alpha
Predicted band size: 110 kDa -
Western blot - Anti-IRE1 (phospho S724) antibody (ab48187)All lanes : Anti-IRE1 (phospho S724) antibody (ab48187) at 2 µg/ml
Lane 1 : Untreated HeLa cells (Batch 1 ab48187)
Lane 2 : DTT-treated HeLa cells (Batch 1 ab48187)
Lane 3 : Untreated HeLa cells (Batch 2 ab48187)
Lane 4 : DTT-treated HeLa cells (Batch 2 ab48187)
Secondary
All lanes : Anti-Rabbit IgG HRP
Predicted band size: 110 kDaHeLa cells were treated (+) or untreated (-) with 10 mM DTT for 60 min to activate the UPR. Total protein was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% BSA in TBST.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (283)
ab48187 は 283 報の論文で使用されています。
- Park SH et al. Ethanol and its Nonoxidative Metabolites Promote Acute Liver Injury by Inducing ER Stress, Adipocyte Death, and Lipolysis. Cell Mol Gastroenterol Hepatol 15:281-306 (2023). PubMed: 36243320
- Qin L et al. NADPH oxidase and endoplasmic reticulum stress is associated with neuronal degeneration in orbitofrontal cortex of individuals with alcohol use disorder. Addict Biol 28:e13262 (2023). PubMed: 36577732
- Solarz-Andrzejewska A et al. Modulation of the endoplasmic reticulum stress and unfolded protein response mitigates the behavioral effects of early-life stress. Pharmacol Rep 75:293-319 (2023). PubMed: 36843201
- Yu J et al. Deficiency of Perry syndrome-associated p150Glued in midbrain dopaminergic neurons leads to progressive neurodegeneration and endoplasmic reticulum abnormalities. NPJ Parkinsons Dis 9:35 (2023). PubMed: 36879021
- Shi D et al. Autophagy is induced by swine acute diarrhea syndrome coronavirus through the cellular IRE1-JNK-Beclin 1 signaling pathway after an interaction of viral membrane-associated papain-like protease and GRP78. PLoS Pathog 19:e1011201 (2023). PubMed: 36888569