AB37073

Anti-IRE1 抗体

Name: Anti-IRE1 抗体 (ab37073) | Abcam
Brand: Abcam Limited
SKU: AB37073
Price: 66000 JPY
Availability: InStock
Rating: 5 (5 reviews)

Anti-IRE1 antibody

(5 Reviews)

(185 Publications)

Anti-IRE1 antibody (ab37073) is a rabbit polyclonal antibody detecting IRE1 in Western Blot, IHC-P. Suitable for Human, Mouse, Rat.

- Over 150 publications
- Trusted since 2006

別名を表示する

IRE1, ERN1, Serine/threonine-protein kinase/endoribonuclease IRE1, Endoplasmic reticulum-to-nucleus signaling 1, Inositol-requiring protein 1, Ire1-alpha, hIRE1p, IRE1a

8 Images

Supplier Data

Western blot - Anti-IRE1 antibody (AB37073)

Diluting buffer and concentration : 1 hour incubation at room temperature in 5% NFDM/TBST.

Beta-actin antibody (1 μg/mL) used as loading control.

All lanes:

Western blot - Anti-IRE1 antibody (ab37073) at 0.5 µg/mL

Lane 1:

Wild-type HeLa Cell Lysate at 10 µg

Lane 2:

IRE1 knockout HeLa Cell Lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) at 1/10000 dilution

Predicted band size: 110 kDa

Observed band size: 110 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRE1 antibody (AB37073)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRE1 antibody (AB37073)

Immunofluorescent analysis of 4% paraformaldehyde fixed Human Small Intestine Tissue labeling IRE1 with ab37073 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRE1 antibody (AB37073)

Immunohistochemical analysis of paraffin-embedded Rat Small Intestine Tissue using ab37073 at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Unknown

Immunocytochemistry - Anti-IRE1 antibody (AB37073)

ab37073, at 1 μg/ml, staining IRE1 in A20 cells with by ICC.

Unknown

Immunocytochemistry - Anti-IRE1 antibody (AB37073)

Immunofluorescence of IRE1p in A20 cells using ab37073 at 2 ug/ml.

Supplier Data

Western blot - Anti-IRE1 antibody (AB37073)

Diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-IRE1 antibody (ab37073) at 0.4 µg/mL

Lane 1:

Caco-2 Cell Lysate at 15 µg

Lane 2:

SK-N-SH Cell Lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) at 1/10000 dilution

Predicted band size: 110 kDa

Observed band size: 110 kDa

false

Unknown

Western blot - Anti-IRE1 antibody (AB37073)

Western Blot of rat brain lysate labeling IRE1 with Anti-IRE1 antibody (ab37073) at (1) 0.5 and (2) 1μg/ml.

All lanes:

Western blot - Anti-IRE1 antibody (ab37073)

Predicted band size: 110 kDa

false

Unknown

Western blot - Anti-IRE1 antibody (AB37073)

Lane 1:

Western blot - Anti-IRE1 antibody (ab37073) at 0.5 µg/mL

Lane 2:

Western blot - Anti-IRE1 antibody (ab37073) at 1 µg/mL

Lane 3:

Western blot - Anti-IRE1 antibody (ab37073) at 2 µg/mL

All lanes:

A20 cell lysate at 15 µg

Secondary

All lanes:

goat anti-rabbit HRP at 1/10000 dilution

Predicted band size: 110 kDa

Observed band size: 107 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

交差種

Mouse, Rat, Human

アプリケーション

WB, ICC, IHC-P

applications

免疫原

Synthetic Peptide within Human ERN1. The exact immunogen used to generate this antibody is proprietary information.

O75460

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2 µg/mL", "IHCP-species-notes": "", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "Please note there is a smaller isoform of IRE1 in rat (predicted molecular weight:68.6kDa; accession number is EDM06401). Block for 2 hours at RT or overnight at 4C using 5% non-fat milk.Primary ab incubation: 1 h incubation at RT in 5% NFDM/TBST." }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2 µg/mL", "IHCP-species-notes": "", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1 µg/mL", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "Please note there is a smaller isoform of IRE1 in rat (predicted molecular weight:68.6kDa; accession number is EDM06401). Block for 2 hours at RT or overnight at 4C using 5% non-fat milk.Primary ab incubation: 1 h incubation at RT in 5% NFDM/TBST." }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2 µg/mL", "IHCP-species-notes": "", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "Please note there is a smaller isoform of IRE1 in rat (predicted molecular weight:68.6kDa; accession number is EDM06401). Block for 2 hours at RT or overnight at 4C using 5% non-fat milk.Primary ab incubation: 1 h incubation at RT in 5% NFDM/TBST." } } }

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製品の詳細

What is this antibody validated in?
Anti-IRE1 antibody (ab37073) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples.

What is the molecular weight of IRE1?
Anti-IRE1 (ab37073) specifically detects a band for IRE1 (UniProt: O75460) at a molecular weight of 110kDa.

Trusted by the scientific community
Anti-IRE1 (ab37073) was first used in a scientific publication in 2006 and has been cited over 150 times in peer-reviewed journals.

Reviewed by scientists
Anti-IRE1 (ab37073) has over 5 independent reviews from customers.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Immunogen

バッファー組成

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The inositol-requiring enzyme 1 (IRE1) also known as ERN1 or IRE1 alpha is a critical endoplasmic reticulum (ER) stress sensor. It has a molecular weight of approximately 110 kDa. IRE1 is expressed in various cell types and tissues particularly in those subject to a high degree of protein synthesis such as the liver pancreas and secretory cells. This protein plays a dual role as both a RNase and a kinase which enables it to respond swiftly to misfolded proteins accumulating in the ER.

Biological function summary

IRE1 is an important regulator in the unfolded protein response (UPR) a cellular reaction to stress in the ER. It operates as part of a complex mechanism facilitating the splicing of X-box binding protein 1 (XBP1) mRNA which results in the production of a potent transcription factor. IRE1 activity helps in restoring normal function of the cell by upregulating genes involved in protein folding secretion and degradation. Its actions are important for maintaining cellular homeostasis during stressful conditions.

Pathways

IRE1 is an integral component of the UPR pathway which works to alleviate ER stress. It interacts closely with other UPR transducers such as activating transcription factor 6 (ATF6) and protein kinase RNA-like ER kinase (PERK). IRE1 connects with the XBP1 pathway facilitating adaptive responses that enhance protein-folding capacity lipid biosynthesis and ER-associated degradation. Altogether these pathways mediate cell survival or apoptosis depending on the severity of the stress.

IRE1 has significant involvement in conditions like diabetes and cancer. In the context of diabetes improper UPR signaling due to chronic ER stress leads to insulin resistance and pancreatic beta-cell dysfunction. In cancer IRE1 modulates tumor microenvironment and promotes cancer cell survival under hypoxic conditions. The XBP1 pathway linked with IRE1 also plays a substantial role in these diseases by influencing cell proliferation and apoptosis. Understanding the mechanisms of IRE1 in these conditions might provide therapeutic insights.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR) (PubMed : 11175748, PubMed : 11779464, PubMed : 12637535, PubMed : 19328063, PubMed : 21317875, PubMed : 28128204, PubMed : 30118681, PubMed : 36739529, PubMed : 9637683). In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP (PubMed : 21317875). Accumulation of misfolded proteins in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity (PubMed : 21317875). The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA (PubMed : 11779464, PubMed : 21317875, PubMed : 24508390). The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes (PubMed : 11779464, PubMed : 21317875, PubMed : 24508390). Acts as an upstream signal for ER stress-induced GORASP2-mediated unconventional (ER/Golgi-independent) trafficking of CFTR to cell membrane by modulating the expression and localization of SEC16A (PubMed : 21884936, PubMed : 28067262).

See full target information ERN1

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文献 (185)

Recent publications for all applications. Explore the full list and refine your search

American journal of translational research 17:6504-6521 PubMed40950286

2025

NLBK alleviates airway remodeling in COPD by inhibiting RXRA-mediated transcription of PLA2G2A.

Applications

Unspecified application

Species

Unspecified reactive species

Teng Zhang,Fang Fang,Dan Wu,Guodong Wang

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 39:e70377 PubMed39985305

2025

Dolutegravir induces endoplasmic reticulum stress at the blood-brain barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Chang Huang,Qing Rui Qu,Md Tozammel Hoque,Reina Bendayan

Bioscience reports 45: PubMed39907066

2025

Hydrogen sulfide attenuates sepsis-induced cardiac dysfunction in infant rats by inhibiting the expression of cold-inducible RNA-binding protein.

Applications

Unspecified application

Species

Unspecified reactive species

Desi Li,Sheng Jin,Xu Teng,Ping Wang,Kaichuan He,Lijing Cao,Jiexian Du,Qi Guo,Lin Xiao,Hongmei Xue,Danyang Tian,Cuixia An,Yuming Wu

Molecular medicine (Cambridge, Mass.) 30:266 PubMed39707212

2024

Osthole ameliorates wear particle-induced osteogenic impairment by mitigating endoplasmic reticulum stress via PERK signaling cascade.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Yu,Juan Jiang,Cheng Li,Yang Wang,Zhengrong Ren,Jianlun Hu,Tao Yuan,Yongjie Wu,Dongsheng Wang,Ziying Sun,Qi Wu,Bin Chen,Peng Fang,Hao Ding,Jia Meng,Hui Jiang,Jianning Zhao,Nirong Bao

American journal of translational research 16:5829-5845 PubMed39544779

2024

Zhi Zhu Ma Ren pill relieves constipation in mice through endoplasmic reticulum stress-mediated apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Wen,Yu Zhan,Jun Li,Ling Xu,Chengzi Huang,Rong Wu,Xuegui Tang

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2403442 PubMed39297413

2024

siRNA/CS-PLGA Nanoparticle System Targeting Knockdown Intestinal SOAT2 Reduced Intestinal Lipid Uptake and Alleviated Obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjia Liang,Wentao Shao,Pu Ni,Qian Liu,Weirui Kong,Weiyi Shen,Qihan Wang,Anhua Huang,Guixin Zhang,Yulong Yang,Hongliang Xin,Zhaoyan Jiang,Aihua Gu

Emerging microbes & infections 13:2399945 PubMed39230190

2024

African swine fever virus modulates the endoplasmic reticulum stress-ATF6-calcium axis to facilitate viral replication.

Applications

Unspecified application

Species

Unspecified reactive species

Yanjin Wang,Jiaqi Li,Hongwei Cao,Lian-Feng Li,Jingwen Dai,Mengxiang Cao,Hao Deng,Dailang Zhong,Yuzi Luo,Yongfeng Li,Meilin Li,Dingkun Peng,Zitao Sun,Xiaowei Gao,Assad Moon,Lijie Tang,Yuan Sun,Su Li,Hua-Ji Qiu

Experimental and therapeutic medicine 28:385 PubMed39161618

2024

Peroxisome proliferator‑activated receptor γ alleviates human umbilical vein endothelial cell injury in deep vein thrombosis by blocking endoplasmic reticulum stress.

Applications

Unspecified application

Species

Unspecified reactive species

Yunxin Zhang,Yongshuai Ge,Liyuan Tao,Mingyuan Liu,Wei Jia,Xuan Tian,Peng Jiang,Zhiyuan Cheng,Jinyong Li,Jianlong Liu

The EMBO journal 43:4846-4869 PubMed39160276

2024

PCPE-1, a brown adipose tissue-derived cytokine, promotes obesity-induced liver fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yung Ting Hsiao,Yohko Yoshida,Shujiro Okuda,Manabu Abe,Seiya Mizuno,Satoru Takahashi,Hironori Nakagami,Ryuichi Morishita,Kenya Kamimura,Shuji Terai,Tin May Aung,Ji Li,Takaaki Furihata,Jing Yuan Tang,Kenneth Walsh,Akihito Ishigami,Tohru Minamino,Ippei Shimizu

International journal of chronic obstructive pulmonary disease 19:1635-1647 PubMed39045541

2024

Cigarette Smoke Extract Induces MUC5AC Expression Through the ROS/ IP3R/Ca Pathway in Calu-3 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojuan Wu,Guoyue Zhang,Xianzhi Du

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

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Anti-IRE1 抗体

Anti-IRE1 antibody

Western blot - Anti-IRE1 antibody (AB37073)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRE1 antibody (AB37073)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRE1 antibody (AB37073)

Immunocytochemistry - Anti-IRE1 antibody (AB37073)

Immunocytochemistry - Anti-IRE1 antibody (AB37073)

Western blot - Anti-IRE1 antibody (AB37073)

Western blot - Anti-IRE1 antibody (AB37073)

Western blot - Anti-IRE1 antibody (AB37073)

Key facts

宿主種

クローン性

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (185)

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