Anti-IRAKM 抗体 (ab8116)
Key features and details
- Rabbit polyclonal to IRAKM
- Suitable for: ICC, IHC-P, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-IRAKM antibody
IRAKM 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IRAKM -
由来種
Rabbit -
特異性
Anti-IRAK-M has no cross response to IRAK or IRAK2. -
アプリケーション
適用あり: ICC, IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat -
免疫原
Synthetic peptide:
CSSKFSWDEYEQYKKE
, corresponding to C terminal amino acids 581-596 of Human IRAKM (Peptide available as ab6231.) -
特記事項
Interleukin-1 (IL-1) and lipopolysaccharide (LPS)induces cellular response through IL-1 receptor (IL-1R) and Toll like receptors (TLR). IL-1 receptor associated kinase (IRAK and IRAK2) mediates the activation of NF-kB by IL-1/Toll receptors (Wesche H et al.1999,Muzio M et al.1997), which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in theIRAK/Pelle family was recently identified and designated IRAK-M (Cao Z et al.1996). IRAKs associate with IL-1/Toll receptors after IL-1 or LPS stimulation and thedominant negative mutants of IRAKs inhibit IL-1 orLPS induced NF-kB activation. Members inIRAK/Pelle family play a central role in IL-1R/TLRmediated inflammatory responses to cytokine IL-1and LPS.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
精製度
Affinity purified -
特記事項（精製）
Purified IgG prepared by ion exchange chromatography. IRAK-M Antibody is affinity chromatography purified via peptide column. -
一次抗体 備考
Interleukin-1 (IL-1) and lipopolysaccharide (LPS)induces cellular response through IL-1 receptor (IL-1R) and Toll like receptors (TLR). IL-1 receptor associated kinase (IRAK and IRAK2) mediates the activation of NF-kB by IL-1/Toll receptors (Wesche H et al.1999,Muzio M et al.1997), which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in theIRAK/Pelle family was recently identified and designated IRAK-M (Cao Z et al.1996). IRAKs associate with IL-1/Toll receptors after IL-1 or LPS stimulation and thedominant negative mutants of IRAKs inhibit IL-1 orLPS induced NF-kB activation. Members inIRAK/Pelle family play a central role in IL-1R/TLRmediated inflammatory responses to cytokine IL-1and LPS. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab8116の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC |
Use at an assay dependent concentration.
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| IHC-P |
Use a concentration of 2 µg/ml.
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| WB | (3) |
Use a concentration of 0.5 - 1 µg/ml.
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| 特記事項 |
|---|
|
ICC
Use at an assay dependent concentration. |
|
IHC-P
Use a concentration of 2 µg/ml. |
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WB
Use a concentration of 0.5 - 1 µg/ml. |
ターゲット情報
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機能
Inhibits dissociation of IRAK1 and IRAK4 from the Toll-like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex. -
組織特異性
Expressed predominantly in peripheral blood lymphocytes. -
関連疾患
Defects in IRAK3 are associated with susceptibility to asthma-related traits type 5 (ASRT5) [MIM:611064]. Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing, dyspnea, bronchial hyperresponsiveness as assessed by methacholine challenge test, serum IgE levels, atopy, and atopic dermatitis. -
配列類似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
Contains 1 death domain.
Contains 1 protein kinase domain. - Information by UniProt
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参照データベース
- Entrez Gene: 73914 Mouse
- Entrez Gene: 314870 Rat
- SwissProt: Q8K4B2 Mouse
- Unigene: 146194 Mouse
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別名
- ASRT5 antibody
- IL 1 receptor associated kinase M antibody
- IL-1 receptor-associated kinase M antibody
see all
画像
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Western blot - Anti-IRAKM antibody (ab8116)All lanes : Anti-IRAKM antibody (ab8116) at 1 µg/ml
Lane 1 : Mouse spleen tissue lysates
Lane 2 : Rat liver tissue lysate
Observed band size: 68 kDa why is the actual band size different from the predicted?
Western blot analysis of IRAKM in mouse spleen (M) and rat liver (R) tissue lysates with anti-IRAKM at 1µ -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAKM antibody (ab8116)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of A20 cells staining IRAKM with ab8116 at 20 µg/ml -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAKM antibody (ab8116)Immunohistochemical staining of rat liver tissue using IRAK-M antibody at 2 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAKM antibody (ab8116)ab8116 at 2µg/ml staining IRAKM in rat liver cells by IHC
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Immunocytochemistry - Anti-IRAKM antibody (ab8116)Image from Lyn-Kew K et al, PLoS One. 2010 Jun 16;5(6):e11145, Fig 4.ab8116 staining IRAKM in murine lung macrophages by Immunocytochemistry/ Immunofluorescence.<.br>
The cells were permeablized with a 1:1 mixture of methanol and acetone. After washing with PBS, ab8116 was applied and the cells incubated for one hour. After washing, antibodies against the primary antibody labeled with FITC were applied to the cells and incubated for one hour. The cell were then mounted in DAPI mounting media and viewed on the fluorescent microscope. -
Immunocytochemistry - Anti-IRAKM antibody (ab8116)Immunofluorescence of IRAK-M in Rat Liver cells using ab8116 at 10 ug/ml.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (15)
ab8116 は 15 報の論文で使用されています。
- Sun J et al. Loss of NLRP3 increases bacterial cystitis via IRAKM. Transl Androl Urol 11:268-276 (2022). PubMed: 35280657
- Hu X & Miao H MiR-539-5p inhibits the inflammatory injury in septic H9c2 cells by regulating IRAK3. Mol Biol Rep 49:121-130 (2022). PubMed: 34757596
- Dienel A et al. α7-Acetylcholine Receptor Signaling Reduces Neuroinflammation After Subarachnoid Hemorrhage in Mice. Neurotherapeutics 18:1891-1904 (2021). PubMed: 33970466
- Cai JL et al. Polygonatum sibiricum polysaccharides (PSP) improve the palmitic acid (PA)-induced inhibition of survival, inflammation, and glucose uptake in skeletal muscle cells. Bioengineered 12:10147-10159 (2021). PubMed: 34872451
- Wu X et al. Hypermethylation of the IRAK3-Activated MAPK Signaling Pathway to Promote the Development of Glioma. Cancer Manag Res 12:7043-7059 (2020). PubMed: 32848462
