Anti-IRAK4 抗体 [EPR24506-71] (BSA and Azide free) (ab300078)
![Western blot - Anti-IRAK4 antibody [EPR24506-71] (BSA and Azide free) (ab300078)](https://www.abcam.co.jp/ps/products/300/ab300078/Images/AB300078-485316-ab300077WB1.jpg "Western blot - Anti-IRAK4 antibody [EPR24506-71] (BSA and Azide free) (ab300078)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24506-71] to IRAK4 - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Mouse, Rat
Related conjugates and formulations
製品の概要
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製品名
Anti-IRAK4 antibody [EPR24506-71] (BSA and Azide free)
IRAK4 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR24506-71] to IRAK4 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details
適用なし: Flow Cyt (Intra),ICC/IF or IHC-P -
種交差性
交差種: Mouse, Rat
非交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Whole cell lysates: RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage), MEF (Mouse embryonic fibroblast), DCS (Mouse dendritic cell sarcoma), 2.4G2(Rat B cell lymphoma B lymphocyte), Mouse lymph node tissue lysate, Rat lymph node tissue lysate.
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特記事項
ab300078 is a carrier free version of ab300077.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR24506-71 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab300078の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa).
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| 特記事項 |
|---|
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IP
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa). |
ターゲット情報
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機能
Required for the efficient recruitment of IRAK1 to the IL-1 receptor complex following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Phosphorylates IRAK1. -
関連疾患
Defects in IRAK4 are the cause of recurrent isolated invasive pneumococcal disease type 1 (IPD1) [MIM:610799]. Recurrent invasive pneumococcal disease (IPD) is defined as two episodes of IPD occurring at least 1 month apart, whether caused by the same or different serotypes or strains. Recurrent IPD occurs in at least 2% of patients in most series, making IPD the most important known risk factor for subsequent IPD.
Defects in IRAK4 are the cause of IRAK4 deficiency (IRAK4D) [MIM:607676]. IRAK4 deficiency causes extracellular pyogenic bacterial and fungal infections in otherwise healthy children. -
配列類似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
Contains 1 death domain.
Contains 1 protein kinase domain. - Information by UniProt
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参照データベース
- Entrez Gene: 266632 Mouse
- Entrez Gene: 300177 Rat
- SwissProt: Q8R4K2 Mouse
- Unigene: 422858 Mouse
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別名
- IL-1 receptor-associated kinase 4 antibody
- Interleukin 1 receptor associated kinase 4 mutant form 1 antibody
- Interleukin-1 receptor-associated kinase 4 antibody
see all
画像
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Western blot - Anti-IRAK4 antibody [EPR24506-71] (BSA and Azide free) (ab300078)All lanes : Anti-IRAK4 antibody [EPR24506-71] (ab300077) at 1/1000 dilution
Lane 1 : RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 2 : MEF (Mouse embryonic fibroblast) whole cell lysate
Lane 3 : DCS (Mouse dendritic cell sarcoma) whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 52 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 70 secondsThis data was developed using ab289711, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: MEF, DCS(PMID:11923871).
The band near 37KD is a nonspecific band.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
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![Western blot - Anti-IRAK4 antibody [EPR24506-71] (BSA and Azide free) (ab300078)](https://www.abcam.co.jp/ps/products/300/ab300078/Images/AB300078-485318-ab300077WB2.jpg)
Western blot - Anti-IRAK4 antibody [EPR24506-71] (BSA and Azide free) (ab300078)All lanes : Anti-IRAK4 antibody [EPR24506-71] (ab300077) at 1/2000 dilution
Lane 1 : RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 2 : 2.4G2 (Rat B cell lymphoma B lymphocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 52 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 81 secondsThis data was developed using ab289711, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Lysates were made freshly and used in WB immediately to minimize protein degradation.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
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![Western blot - Anti-IRAK4 antibody [EPR24506-71] (BSA and Azide free) (ab300078)](https://www.abcam.co.jp/ps/products/300/ab300078/Images/AB300078-485320-ab300077WB3.jpg)
Western blot - Anti-IRAK4 antibody [EPR24506-71] (BSA and Azide free) (ab300078)All lanes : Anti-IRAK4 antibody [EPR24506-71] (ab300077) at 1/1000 dilution
Lane 1 : Mouse lymph node tissue lysate
Lane 2 : Rat lymph node tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 52 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 70 secondsThis data was developed using ab289711, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The band near 37KD is a nonspecific band.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
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![Immunoprecipitation - Anti-IRAK4 antibody [EPR24506-71] (BSA and Azide free) (AB300078)](https://www.abcam.co.jp/ps/products/300/ab300078/Images/AB300078-5-anti-irak4-antibody-epr2450671-immunoprecipitation-mef.jpg)
Immunoprecipitation - Anti-IRAK4 antibody [EPR24506-71] (BSA and Azide free) (AB300078)This data was developed using ab300077, the same antibody clone in a different buffer formulation.
IRAK4 was immunoprecipitated from 0.35 mg MEF (mouse embryonic fibroblast) whole cell lysate 10µg with ab300077 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300077 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: MEF (mouse embryonic fibroblast) whole cell lysate 10µg
Lane 2: ab300077 in MEF whole cell lysate 10µg
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab300077 in MEF whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 180 seconds
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![Rabbit monoclonal [EPR24506-71] to IRAK4 - BSA and Azide free (AB300078)](https://www.abcam.co.jp/ps/products/300/ab300078/Images/AB300078-485274-Benefits-of-Recombinant-antibodies.jpg)
Rabbit monoclonal [EPR24506-71] to IRAK4 - BSA and Azide free (AB300078)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab300078 は論文での使用が確認できていません。
