Anti-IRAK4 抗体 (ab5985)
Key features and details
- Rabbit polyclonal to IRAK4
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-IRAK4 antibody
IRAK4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IRAK4 -
由来種
Rabbit -
特異性
This antibody is specific to IRAK4 and does not cross react with other IRAK proteins. -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human IRAK4 (C terminal).
(Peptide available asab39782) -
ポジティブ･コントロール
- WB: Daudi, HepG2 and HeLa whole cell lysate. ICC/IF: K562 cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. The product can be stored for up to 12 months. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab5985の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 10 µg/ml.
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| WB | (1) |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 50 kDa.
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| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 10 µg/ml. |
|
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 50 kDa. |
ターゲット情報
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機能
Required for the efficient recruitment of IRAK1 to the IL-1 receptor complex following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Phosphorylates IRAK1. -
関連疾患
Defects in IRAK4 are the cause of recurrent isolated invasive pneumococcal disease type 1 (IPD1) [MIM:610799]. Recurrent invasive pneumococcal disease (IPD) is defined as two episodes of IPD occurring at least 1 month apart, whether caused by the same or different serotypes or strains. Recurrent IPD occurs in at least 2% of patients in most series, making IPD the most important known risk factor for subsequent IPD.
Defects in IRAK4 are the cause of IRAK4 deficiency (IRAK4D) [MIM:607676]. IRAK4 deficiency causes extracellular pyogenic bacterial and fungal infections in otherwise healthy children. -
配列類似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
Contains 1 death domain.
Contains 1 protein kinase domain. - Information by UniProt
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参照データベース
- Entrez Gene: 51135 Human
- Omim: 606883 Human
- SwissProt: Q9NWZ3 Human
- Unigene: 138499 Human
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別名
- IL-1 receptor-associated kinase 4 antibody
- Interleukin 1 receptor associated kinase 4 mutant form 1 antibody
- Interleukin-1 receptor-associated kinase 4 antibody
see all
画像
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Western blot - Anti-IRAK4 antibody (ab5985)All lanes : Anti-IRAK4 antibody (ab5985) at 1 µg/ml
Lane 1 : Daudi cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution -
Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (ab5985)Immunofluorescent analysis of 4% paraformaldehydefixed K562 Cells labeling IRAK4 with ab9585 at 10 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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Western blot - Anti-IRAK4 antibody (ab5985)Lane 1 : Anti-IRAK4 antibody (ab5985) at 1 µg/ml
Lane 2 : Anti-IRAK4 antibody (ab5985) at 2 µg/ml
Lane 3 : Anti-IRAK4 antibody (ab5985) at 4 µg/ml
All lanes : HeLa cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilutionPrimary incbation: 1 hour at room temperature in 5% NFDM/TBST.
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Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (ab5985)Immunocytochemical analysis of K562 cells labeling IRAK4 with ab5985 at 10 μg/mL. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody (ab5985)This image was kindly supplied by Dr Mahesh Shivananjappa by Abreviewab5985 at a 1/100 dilution staining IRAK4 in human PMN cells by Immunocytochemistry/ Immunofluorescence incubated for 4 hours at 37°C. Fixed with PFA. Blocked using 2% BSA for 1 hour at 22°C. Secondary used at 1/250 polyclonal Goat anti-rabbit conjugated to Alexa Fluor 568.
Left image: DAPI staining nuclei (blue)Middle image: IRAK4 (red)
Right image: Overlay
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab5985 は 4 報の論文で使用されています。
- Choudhary GS et al. Activation of targetable inflammatory immune signaling is seen in myelodysplastic syndromes with SF3B1 mutations. Elife 11:N/A (2022). PubMed: 36040792
- Shi C et al. lncRNA SNHG14 Plays a Role in Sepsis-Induced Acute Kidney Injury by Regulating miR-93. Mediators Inflamm 2021:5318369 (2021). PubMed: 33505213
- Smith MA et al. U2AF1 mutations induce oncogenic IRAK4 isoforms and activate innate immune pathways in myeloid malignancies. Nat Cell Biol 21:640-650 (2019). PubMed: 31011167
- Kallapur SG et al. Pulmonary and systemic endotoxin tolerance in preterm fetal sheep exposed to chorioamnionitis. J Immunol 179:8491-9 (2007). WB ; Sheep . PubMed: 18056396