Anti-IRAK2 抗体 (ab62419)
Key features and details
- Rabbit polyclonal to IRAK2
- Suitable for: ICC/IF, IP, ICC, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-IRAK2 antibody
IRAK2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IRAK2 -
由来種
Rabbit -
特異性
This antibody reacts with IRAK2, it does not react with IRAK -
アプリケーション
適用あり: ICC/IF, IP, ICC, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
A synthetic peptide corresponding to 17 amino acids near the C terminus of mouse IRAK2a, (which is common to all four isoforms).
-
ポジティブ･コントロール
- WB: RAW264.7 whole cell lysate. ICC: A-20 cells. ICC/IF: A-20 cells.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab62419の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 20 µg/ml.
|
|
| IP | (1) |
Use at an assay dependent concentration.
(PMID 19224918) |
| ICC |
Use a concentration of 1 µg/ml.
|
|
| WB | (2) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
|
| 特記事項 |
|---|
|
ICC/IF
Use a concentration of 20 µg/ml. |
|
IP
Use at an assay dependent concentration. (PMID 19224918) |
|
ICC
Use a concentration of 1 µg/ml. |
|
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa). |
ターゲット情報
-
機能
Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. -
組織特異性
Expressed in spleen, thymus, prostate, lung, liver, skeletal muscle, kidney, pancreas and peripheral blood leukocytes. -
配列類似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
Contains 1 death domain.
Contains 1 protein kinase domain. -
ドメイン
The protein kinase domain is predicted to be catalytically inactive. - Information by UniProt
-
参照データベース
- Entrez Gene: 3656 Human
- Entrez Gene: 108960 Mouse
- Omim: 603304 Human
- SwissProt: O43187 Human
- SwissProt: Q8CFA1 Mouse
- Unigene: 449207 Human
- Unigene: 152142 Mouse
-
別名
- il1rak2 antibody
- Interleukin 1 receptor associated kinase 2 antibody
- Interleukin 1 receptor associated kinase like 2 antibody
see all
画像
-
Western blot - Anti-IRAK2 antibody (ab62419)This image is courtesy of an anonymous abreview.
Anti-IRAK antibody (ab62419) reactivity with reduced wildtype (WT) or IRAK2 knockout (KO) mouse embryonic fibroblast (MEF) cell lysate. After SDS-PAGE, membranes were blocked in 5% milk for 1h at 25°C before incubation with ab62419 (1µg/ml dilution in 5% milk in TBS/0.5% Tween) for 16h at 4ºC. The blot was then incubated with an anti-Rabbit HRP-conjugated secondary antibody before developing with ECL.
-
Western blot - Anti-IRAK2 antibody (ab62419)Lane 1 : Anti-IRAK2 antibody (ab62419) at 0.5 µg/ml
Lane 2 : Anti-IRAK2 antibody (ab62419) at 1 µg/ml
Lane 3 : Anti-IRAK2 antibody (ab62419) at 2 µg/ml
Lane 1 : RAW264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lanes 2-3 : RAW264.7 whole cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 65 kDa
Observed band size: 65 kDa -
Immunocytochemistry - Anti-IRAK2 antibody (ab62419)Immunocytochemistry of IRAK2 in A-20 cells with ab62419 antibody at 1 µg/ml. -
Immunocytochemistry/ Immunofluorescence - Anti-IRAK2 antibody (ab62419)Immunofluorescence of IRAK2 in A20 cells using ab62419 at 10 ug/ml.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (13)
ab62419 は 13 報の論文で使用されています。
- Liu W et al. IL-1R-IRAKM-Slc25a1 signaling axis reprograms lipogenesis in adipocytes to promote diet-induced obesity in mice. Nat Commun 13:2748 (2022). PubMed: 35585086
- McCrory EH et al. Identification of ester-linked ubiquitylation sites during TLR7 signalling increases the number of inter-ubiquitin linkages from 8 to 12. Biochem J 479:2419-2431 (2022). PubMed: 36408944
- Xiao T et al. lncRNA NEAT1 mediates sepsis progression by regulating Irak2 via sponging miR-370-3p. Biol Open 9:N/A (2020). PubMed: 32414769
- Zhou H et al. IL-1 induces mitochondrial translocation of IRAK2 to suppress oxidative metabolism in adipocytes. Nat Immunol 21:1219-1231 (2020). PubMed: 32778760
- Zhou H et al. IRAK2 directs stimulus-dependent nuclear export of inflammatory mRNAs. Elife 6:N/A (2017). PubMed: 28990926