Anti-IQGAP2 抗体 [EPR13382-9] (ab187153)

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Abpromise

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP2 antibody [EPR13382-9] (ab187153)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13382-9] to IQGAP2
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Carrier Free

製品名

Anti-IQGAP2 antibody [EPR13382-9]
IQGAP2 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR13382-9] to IQGAP2
由来種

Rabbit
特異性

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
アプリケーション

適用あり: WB, IHC-Pmore details
種交差性

交差種: Mouse, Rat, Human
免疫原

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: HepG2 and Ramos cell lysate, Rat liver and Mouse testis tissue lysate. IHC-P: Human prostatic hyperplasia, colonic carcinoma and Mouse testis tissue.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
バッファー

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR13382-9
アイソタイプ

IgG
研究分野

Alternative Versions
- Anti-IQGAP2 antibody [EPR13382-9] - BSA and Azide free (ab233030)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab187153の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
WB		1/1000 - 1/10000. Predicted molecular weight: 181 kDa.
IHC-P		1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 181 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

機能

Binds to activated CDC42 and RAC1 but does not seem to stimulate their GTPase activity. Associates with calmodulin.
組織特異性

Isoform 2 expression is enhanced in testis.
配列類似性

Contains 1 CH (calponin-homology) domain.
Contains 3 IQ domains.
Contains 1 Ras-GAP domain.
Contains 1 WW domain.
Target information above from: UniProt accession Q13576 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 10788 Human
- Entrez Gene: 544963 Mouse
- Entrez Gene: 100360623 Rat
- Omim: 605401 Human
- SwissProt: Q13576 Human
- SwissProt: Q3UQ44 Mouse
- Unigene: 291030 Human
- Unigene: 38878 Mouse
別名
- IQ motif containing GTPase activating protein 2 antibody
- IQGA2_HUMAN antibody
- Iqgap2 antibody
- Ras GTPase activating-like protein antibody
- Ras GTPase-activating-like protein IQGAP2 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP2 antibody [EPR13382-9] (ab187153)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostatic hyperplasia tissue sections labeling IQGAP2 with Purified ab187153 at 1:100 dilution (1.04 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
Western blot - Anti-IQGAP2 antibody [EPR13382-9] (ab187153)

All lanes : Anti-IQGAP2 antibody [EPR13382-9] (ab187153) at 0.1 µg/ml (purified)

Lane 1 : Rat liver lysates
Lane 2 : Mouse testis lysates

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 181 kDa

Blocking and diluting buffer: 5% NFDM/TBST.
Western blot - Anti-IQGAP2 antibody [EPR13382-9] (ab187153)

Anti-IQGAP2 antibody [EPR13382-9] (ab187153) at 0.01 µg/ml (purified) + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 181 kDa

Blocking and diluting buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP2 antibody [EPR13382-9] (ab187153)

Immunohistochemical analysis of paraffin-embedded Mouse testis labeling IQGAP2 with unpurified ab187153 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP2 antibody [EPR13382-9] (ab187153)

Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma labeling IQGAP2 with unpurified ab187153 at 1/100 dilution.
Western blot - Anti-IQGAP2 antibody [EPR13382-9] (ab187153)

All lanes : Anti-IQGAP2 antibody [EPR13382-9] (ab187153) at 1/1000 dilution (Unpurified)

Lane 1 : HepG2 cell lysate
Lane 2 : Ramos cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 181 kDa
Anti-IQGAP2 antibody [EPR13382-9] (ab187153)

Western blot protocols
Immunohistochemistry protocols

Click here to view the general protocols

SDS download

Country/Region

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Datasheet download

Download

ab187153 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab187153 は 1 報の論文で使用されています。

Wang D et al. LncRNA PDCD4-AS1 alleviates triple negative breast cancer by increasing expression of IQGAP2 via miR-10b-5p. Transl Oncol 14:100958 (2021). PubMed: 33248413

レビューと Q&A

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Key features and details

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製品の概要

製品名

製品の詳細

由来種

特異性

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Compatible Secondaries

Isotype control

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

配列類似性

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

Certificate of Compliance

参考文献 (1)

レビューと Q&A