Anti-IQGAP1 抗体 (ab86064)
Key features and details
- Rabbit polyclonal to IQGAP1
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-IQGAP1 antibody
IQGAP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IQGAP1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Ferret, Rhesus monkey, Gorilla, Elephant
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免疫原
Synthetic peptide, corresponding to a region between residues 1 and 50 of human IQGAP1 (NP_003861.1)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab86064 was affinity purified using an epitope specific to IQGAP1 immobilized on solid suppo -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab86064の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | (1) |
Use at an assay dependent concentration.
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| WB | (3) |
1/2000 - 1/10000. Detects a band of approximately 189 kDa (predicted molecular weight: 189 kDa).
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| IP |
Use at 2-5 µg/mg of lysate.
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| 特記事項 |
|---|
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IHC-P
Use at an assay dependent concentration. |
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WB
1/2000 - 1/10000. Detects a band of approximately 189 kDa (predicted molecular weight: 189 kDa). |
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IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. May promote neurite outgrowth. -
組織特異性
Expressed in the placenta, lung, and kidney. A lower level expression is seen in the heart, liver, skeletal muscle and pancreas. -
配列類似性
Contains 1 CH (calponin-homology) domain.
Contains 4 IQ domains.
Contains 1 Ras-GAP domain.
Contains 1 WW domain. -
ドメイン
Regions C1 and C2 can either interact with nucleotide-free CDC42, or interact together, depending on the phosphorylation state of Ser-1443. When Ser-1443 is not phosphorylated, C1 and C2 interact, which prevents binding of nucleotide-free CDC42 and promotes binding of GTP-bound CDC42. Phosphorylation of Ser-1443 prevents interaction between C1 and C2, which opens the structure of the C-terminus and allows binding and sequestration of nucleotide-free CDC42 on both C1 and C2. -
翻訳後修飾
Phosphorylation of Ser-1443 by PKC prevents interaction between C1 and C2, allowing binding of nucleotide-free CDC42. Ser-1443 phosphorylation enhances the ability to promote neurite outgrowth. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 8826 Human
- Omim: 603379 Human
- SwissProt: P46940 Human
- Unigene: 430551 Human
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別名
- HUMORFA01 antibody
- IQ motif containing GTPase activating protein 1 antibody
- IQGA1_HUMAN antibody
see all
画像
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Western blot - Anti-IQGAP1 antibody (ab86064)All lanes : Anti-IQGAP1 antibody (ab86064) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 189 kDa
Observed band size: 189 kDa
Additional bands at: 238 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP1 antibody (ab86064)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling IQGAP1 with ab86064 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection: DAB and a DyLight® 594-conjugated goat anti-rabbit IgG (H+L) (1/100). -
Immunoprecipitation - Anti-IQGAP1 antibody (ab86064)Detection of IQGAP1 by Western Blot of Immunprecipitate.
ab86064 at 0.4µg/ml staining IQGAP1 in HeLa whole cell lysate immunoprecipitated using ab86064 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP1 antibody (ab86064)Image courtesy of an anonymous Abreview.ab86064 staining IQGAP1 in human pacreatic islets by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed in paraformaldehyde and a heat mediated antigen retrieval step was performed using citrate. Samples were then blocked with 5% serum for 1 hour at 20°C and then incubated with ab86064 at a 1/100 dilution for 8 hours at 4°C. The secondary used was a biotin conjugated goat anti-rabbit polyclonal used at a 1/1000 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (13)
ab86064 は 13 報の論文で使用されています。
- Fang H et al. Integrin β4 promotes DNA damage-related drug resistance in triple-negative breast cancer via TNFAIP2/IQGAP1/RAC1. Elife 12:N/A (2023). PubMed: 37787041
- Bian X et al. AMD1 upregulates hepatocellular carcinoma cells stemness by FTO mediated mRNA demethylation. Clin Transl Med 11:e352 (2021). PubMed: 33783988
- Mo CF et al. IQGAP1 promotes anoikis resistance and metastasis through Rac1-dependent ROS accumulation and activation of Src/FAK signalling in hepatocellular carcinoma. Br J Cancer 123:1154-1163 (2020). PubMed: 32632148
- Chellini L et al. Regulation of extracellular matrix degradation and metastatic spread by IQGAP1 through endothelin-1 receptor signalling in ovarian cancer. Matrix Biol 81:17-33 (2019). PubMed: 30367951
- Li JH et al. IQGAP1 Maintains Pancreatic Ductal Adenocarcinoma Clonogenic Growth and Metastasis. Pancreas 48:94-98 (2019). PubMed: 30540680