Anti-IP6K2 抗体 (ab179921)
Key features and details
- Rabbit polyclonal to IP6K2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG
製品の概要
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製品名
Anti-IP6K2 antibody
IP6K2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to IP6K2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human, African green monkey -
免疫原
Synthetic peptide corresponding to Human IP6K2 (internal sequence).
Database link: Q9UHH9 -
ポジティブ･コントロール
- IHC-P: Human small intestine tissue. WB: Jurkat, COLO and HUVEC cell lysates. ICC/IF: COS7 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine), 49% PBS
PBS without Mg2+, Ca2+ -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab179921の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 49 kDa.
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| IHC-P |
Use a concentration of 20 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/500.
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| 特記事項 |
|---|
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WB
1/500 - 1/1000. Predicted molecular weight: 49 kDa. |
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IHC-P
Use a concentration of 20 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/500. |
ターゲット情報
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機能
Converts inositol hexakisphosphate (InsP6) to diphosphoinositol pentakisphosphate (InsP7/PP-InsP5). Converts 1,3,4,5,6-pentakisphosphate (InsP5) to PP-InsP4. -
配列類似性
Belongs to the inositol phosphokinase (IPK) family. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 51447 Human
- Omim: 606992 Human
- SwissProt: Q9UHH9 Human
- Unigene: 595983 Human
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別名
- ATP 1D myo inositol hexakisphosphate phosphotransferase antibody
- IHPK 2 antibody
- IHPK2 antibody
see all
画像
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Western blot - Anti-IP6K2 antibody (ab179921)All lanes : Anti-RFPL1 antibody (ab172291) at 1/500 dilution
Lane 1 : HUVEC cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : COLO cell lysate
Lane 4 : COLO cell lysate with Immunizing peptide
Predicted band size: 49 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP6K2 antibody (ab179921)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human small intestine tissue labeling IP6K2 with ab179921 at 20 µg/ml.
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Immunocytochemistry/ Immunofluorescence - Anti-IP6K2 antibody (ab179921)Immunofluorescent analysis of COS-7 (African green monkey kidney fibroblast-like cell line) cells labeling IP6K2 with ab179921 at 1/100 dilution. The image on the right is treated with the synthesized peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab179921 は 1 報の論文で使用されています。
- Zhang Y et al. LINC00467 facilitates the proliferation, migration and invasion of glioma via promoting the expression of inositol hexakisphosphate kinase 2 by binding to miR-339-3p. Bioengineered 13:3370-3382 (2022). PubMed: 35156508